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Influence of copper-alloying of austenitic stainless steel on multi-species biofilm development

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Abstract
Aims: To investigate the bactericidal influence of copper-alloying of stainless steel on microbial colonization. Methods and Results: Inhibition of bacterial adherence was investigated by monitoring (192 h) the development of a multi-species biofilm on Cu-alloyed (3.72 wt%) stainless steel in a natural surface water. During the first 120 h of exposure, lower numbers of viable bacteria in the water in contact with copper-containing steel relative to ordinary stainless steel were observed. Moreover, during the first 48 h of exposure, lower colony counts were found in the biofilm adhering to the Cu-alloyed steel. No lower colony or viable counts were found throughout the remainder of the experimental period. Conclusions: The presence of Cu in the steel matrix impedes the adhesion of micro-organisms during an initial period (48 h), while this bactericidal effect disappears after longer incubation periods. Significance and Impact of the Study: The application of Cu-alloyed stainless steels for bactericidal purposes should be restricted to regularly-cleaned surfaces.
Keywords
COLONIZATION, WATER, CORROSION

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MLA
Kielemoes, Jan, and Willy Verstraete. “Influence of Copper-Alloying of Austenitic Stainless Steel on Multi-Species Biofilm Development.” LETTERS IN APPLIED MICROBIOLOGY, vol. 33, no. 2, 2001, pp. 148–52, doi:10.1046/j.1472-765x.2001.00967.x.
APA
Kielemoes, J., & Verstraete, W. (2001). Influence of copper-alloying of austenitic stainless steel on multi-species biofilm development. LETTERS IN APPLIED MICROBIOLOGY, 33(2), 148–152. https://doi.org/10.1046/j.1472-765x.2001.00967.x
Chicago author-date
Kielemoes, Jan, and Willy Verstraete. 2001. “Influence of Copper-Alloying of Austenitic Stainless Steel on Multi-Species Biofilm Development.” LETTERS IN APPLIED MICROBIOLOGY 33 (2): 148–52. https://doi.org/10.1046/j.1472-765x.2001.00967.x.
Chicago author-date (all authors)
Kielemoes, Jan, and Willy Verstraete. 2001. “Influence of Copper-Alloying of Austenitic Stainless Steel on Multi-Species Biofilm Development.” LETTERS IN APPLIED MICROBIOLOGY 33 (2): 148–152. doi:10.1046/j.1472-765x.2001.00967.x.
Vancouver
1.
Kielemoes J, Verstraete W. Influence of copper-alloying of austenitic stainless steel on multi-species biofilm development. LETTERS IN APPLIED MICROBIOLOGY. 2001;33(2):148–52.
IEEE
[1]
J. Kielemoes and W. Verstraete, “Influence of copper-alloying of austenitic stainless steel on multi-species biofilm development,” LETTERS IN APPLIED MICROBIOLOGY, vol. 33, no. 2, pp. 148–152, 2001.
@article{169284,
  abstract     = {{Aims: To investigate the bactericidal influence of copper-alloying of stainless steel on microbial colonization.
Methods and Results: Inhibition of bacterial adherence was investigated by monitoring (192 h) the development of a multi-species biofilm on Cu-alloyed (3.72 wt%) stainless steel in a natural surface water. During the first 120 h of exposure, lower numbers of viable bacteria in the water in contact with copper-containing steel relative to ordinary stainless steel were observed. Moreover, during the first 48 h of exposure, lower colony counts were found in the biofilm adhering to the Cu-alloyed steel. No lower colony or viable counts were found throughout the remainder of the experimental period.
Conclusions: The presence of Cu in the steel matrix impedes the adhesion of micro-organisms during an initial period (48 h), while this bactericidal effect disappears after longer incubation periods.
Significance and Impact of the Study: The application of Cu-alloyed stainless steels for bactericidal purposes should be restricted to regularly-cleaned surfaces.}},
  author       = {{Kielemoes, Jan and Verstraete, Willy}},
  issn         = {{0266-8254}},
  journal      = {{LETTERS IN APPLIED MICROBIOLOGY}},
  keywords     = {{COLONIZATION,WATER,CORROSION}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{148--152}},
  title        = {{Influence of copper-alloying of austenitic stainless steel on multi-species biofilm development}},
  url          = {{http://doi.org/10.1046/j.1472-765x.2001.00967.x}},
  volume       = {{33}},
  year         = {{2001}},
}

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