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Gastrodianin-like mannose-binding proteins: a novel class of plant proteins with antifungal properties

Xiaochen Wang, Guy Bauw UGent, Els Van Damme UGent, Willy J Peumans, Zhang-Liang Chen, Marc Van Montagu UGent, Geert Angenon UGent and Willy Dillen UGent (2001) PLANT JOURNAL. 25(6). p.651-661
abstract
The orchid Gastrodia elata depends on the fungus Armillaria mellea to complete its life cycle. In the interaction, fungal hyphae penetrate older, nutritive corms but not newly formed corms. From these corms, a protein fraction with in vitro activity against plant-pathogenic fungi has previously been purified. Here, the sequence of gastrodianin, the main constituent of the antifungal fraction, is reported. Four isoforms that encoded two different mature proteins were identified at the cDNA level. Another isoform was detected in sequenced peptides. Because the antifungal activity of gastrodianins produced in and purified from Escherichia coil and Nicotiana tabacum was comparable to that of gastrodianin purified from the orchid, gastrodianins are the active component of the antifungal fractions. Gastrodianin accumulation is probably an important part of the mechanism by which the orchid controls Armillaria penetration. Gastrodianin was found to be homologous to monomeric mannose-binding proteins of other orchids, of which at least one (Epipactis helleborine mannose-binding protein) also displayed in vitro antifungal activity. This establishes the gastrodianin-like proteins (GLIPs) as a novel class of antifungal proteins.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
MIRABILIS-JALAPA, EPIPACTIS-HELLEBORINE, ESCHERICHIA-COLI, ACTIN-BINDING, FUNGAL GROWTH, ENHANCED RESISTANCE, ANTIMICROBIAL PEPTIDES, MOLECULAR-CLONING, SPECIES LISTERA-OVATA, TRANSGENIC TOBACCO PLANTS, Orchidaceae, mannose-binding protein, lectin, Gastrodia elata, antifungal protein, Epipactis helleborine
journal title
PLANT JOURNAL
Plant J.
volume
25
issue
6
pages
651 - 661
Web of Science type
Article
Web of Science id
000168584800006
JCR category
PLANT SCIENCES
JCR impact factor
5.792 (2001)
JCR rank
5/134 (2001)
JCR quartile
1 (2001)
ISSN
0960-7412
DOI
10.1046/j.1365-313x.2001.00999.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
168738
handle
http://hdl.handle.net/1854/LU-168738
date created
2004-01-14 13:40:00
date last changed
2016-12-19 15:37:55
@article{168738,
  abstract     = {The orchid Gastrodia elata depends on the fungus Armillaria mellea to complete its life cycle. In the interaction, fungal hyphae penetrate older, nutritive corms but not newly formed corms. From these corms, a protein fraction with in vitro activity against plant-pathogenic fungi has previously been purified. Here, the sequence of gastrodianin, the main constituent of the antifungal fraction, is reported. Four isoforms that encoded two different mature proteins were identified at the cDNA level. Another isoform was detected in sequenced peptides. Because the antifungal activity of gastrodianins produced in and purified from Escherichia coil and Nicotiana tabacum was comparable to that of gastrodianin purified from the orchid, gastrodianins are the active component of the antifungal fractions. Gastrodianin accumulation is probably an important part of the mechanism by which the orchid controls Armillaria penetration. Gastrodianin was found to be homologous to monomeric mannose-binding proteins of other orchids, of which at least one (Epipactis helleborine mannose-binding protein) also displayed in vitro antifungal activity. This establishes the gastrodianin-like proteins (GLIPs) as a novel class of antifungal proteins.},
  author       = {Wang, Xiaochen and Bauw, Guy and Van Damme, Els and Peumans, Willy J and Chen, Zhang-Liang and Van Montagu, Marc and Angenon, Geert and Dillen, Willy},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {MIRABILIS-JALAPA,EPIPACTIS-HELLEBORINE,ESCHERICHIA-COLI,ACTIN-BINDING,FUNGAL GROWTH,ENHANCED RESISTANCE,ANTIMICROBIAL PEPTIDES,MOLECULAR-CLONING,SPECIES LISTERA-OVATA,TRANSGENIC TOBACCO PLANTS,Orchidaceae,mannose-binding protein,lectin,Gastrodia elata,antifungal protein,Epipactis helleborine},
  language     = {eng},
  number       = {6},
  pages        = {651--661},
  title        = {Gastrodianin-like mannose-binding proteins: a novel class of plant proteins with antifungal properties},
  url          = {http://dx.doi.org/10.1046/j.1365-313x.2001.00999.x},
  volume       = {25},
  year         = {2001},
}

Chicago
Wang, Xiaochen, Guy Bauw, Els Van Damme, Willy J Peumans, Zhang-Liang Chen, Marc Van Montagu, Geert Angenon, and Willy Dillen. 2001. “Gastrodianin-like Mannose-binding Proteins: a Novel Class of Plant Proteins with Antifungal Properties.” Plant Journal 25 (6): 651–661.
APA
Wang, Xiaochen, Bauw, G., Van Damme, E., Peumans, W. J., Chen, Z.-L., Van Montagu, M., Angenon, G., et al. (2001). Gastrodianin-like mannose-binding proteins: a novel class of plant proteins with antifungal properties. PLANT JOURNAL, 25(6), 651–661.
Vancouver
1.
Wang X, Bauw G, Van Damme E, Peumans WJ, Chen Z-L, Van Montagu M, et al. Gastrodianin-like mannose-binding proteins: a novel class of plant proteins with antifungal properties. PLANT JOURNAL. 2001;25(6):651–61.
MLA
Wang, Xiaochen, Guy Bauw, Els Van Damme, et al. “Gastrodianin-like Mannose-binding Proteins: a Novel Class of Plant Proteins with Antifungal Properties.” PLANT JOURNAL 25.6 (2001): 651–661. Print.