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Use of the Verrucomicrobia-specific probe EUB338-III and fluorescent in situ hybridization for detection of 'Candidatus Xiphinematobacter' cells in nematode hosts

Tom Vandekerckhove UGent, August Coomans, Karen Cornelis, Philippe Baert and Monique Gillis (2002) APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 68(6). p.3121-3125
abstract
Fluorescent in situ hybridization with a 16S rRNA probe specific for Verrucomicrobia was used to (i) confirm the division-level identity of and (ii) study the behavior of the obligate intracellular verrucomicrobium "Candidatus Xiphinematobacter" in its nematode hosts. Endosymbionts in the egg move to the pole where the gut primordium arises; hence, they populate the intestinal epithelia of juvenile worms. During the host's molt to adult female, the endosymbionts concentrate around the developing ovaries to occupy the ovarian wall. Some bacteria are enclosed in the ripening oocytes for vertical transmission. Verrucomicrobia in males stay outside the testes because the tiny spermatozoids are not suitable for transmission of cytoplasmic bacteria.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
BACTERIA, OLIGONUCLEOTIDE PROBES, LONGIDORIDAE, PROKARYOTES, SYMBIONTS
journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Appl. Environ. Microbiol.
volume
68
issue
6
pages
3121 - 3125
Web of Science type
Article
Web of Science id
000176030100063
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.691 (2002)
JCR rank
16/130 (2002)
JCR quartile
1 (2002)
ISSN
0099-2240
DOI
10.1128/AEM.68.6.3121-3125.2002
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
163081
handle
http://hdl.handle.net/1854/LU-163081
date created
2004-01-14 13:39:00
date last changed
2016-12-19 15:38:23
@article{163081,
  abstract     = {Fluorescent in situ hybridization with a 16S rRNA probe specific for Verrucomicrobia was used to (i) confirm the division-level identity of and (ii) study the behavior of the obligate intracellular verrucomicrobium {\textacutedbl}Candidatus Xiphinematobacter{\textacutedbl} in its nematode hosts. Endosymbionts in the egg move to the pole where the gut primordium arises; hence, they populate the intestinal epithelia of juvenile worms. During the host's molt to adult female, the endosymbionts concentrate around the developing ovaries to occupy the ovarian wall. Some bacteria are enclosed in the ripening oocytes for vertical transmission. Verrucomicrobia in males stay outside the testes because the tiny spermatozoids are not suitable for transmission of cytoplasmic bacteria.},
  author       = {Vandekerckhove, Tom and Coomans, August and Cornelis, Karen and Baert, Philippe and Gillis, Monique},
  issn         = {0099-2240},
  journal      = {APPLIED AND ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {BACTERIA,OLIGONUCLEOTIDE PROBES,LONGIDORIDAE,PROKARYOTES,SYMBIONTS},
  language     = {eng},
  number       = {6},
  pages        = {3121--3125},
  title        = {Use of the Verrucomicrobia-specific probe EUB338-III and fluorescent in situ hybridization for detection of 'Candidatus Xiphinematobacter' cells in nematode hosts},
  url          = {http://dx.doi.org/10.1128/AEM.68.6.3121-3125.2002},
  volume       = {68},
  year         = {2002},
}

Chicago
Vandekerckhove, Tom, August Coomans, Karen Cornelis, Philippe Baert, and Monique Gillis. 2002. “Use of the Verrucomicrobia-specific Probe EUB338-III and Fluorescent in Situ Hybridization for Detection of ‘Candidatus Xiphinematobacter’ Cells in Nematode Hosts.” Applied and Environmental Microbiology 68 (6): 3121–3125.
APA
Vandekerckhove, T., Coomans, A., Cornelis, K., Baert, P., & Gillis, M. (2002). Use of the Verrucomicrobia-specific probe EUB338-III and fluorescent in situ hybridization for detection of “Candidatus Xiphinematobacter” cells in nematode hosts. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(6), 3121–3125.
Vancouver
1.
Vandekerckhove T, Coomans A, Cornelis K, Baert P, Gillis M. Use of the Verrucomicrobia-specific probe EUB338-III and fluorescent in situ hybridization for detection of “Candidatus Xiphinematobacter” cells in nematode hosts. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2002;68(6):3121–5.
MLA
Vandekerckhove, Tom, August Coomans, Karen Cornelis, et al. “Use of the Verrucomicrobia-specific Probe EUB338-III and Fluorescent in Situ Hybridization for Detection of ‘Candidatus Xiphinematobacter’ Cells in Nematode Hosts.” APPLIED AND ENVIRONMENTAL MICROBIOLOGY 68.6 (2002): 3121–3125. Print.