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Direct determination of methylmercury and inorganic mercury in biological materials by solid sampling-electrothermal vaporization-inductively coupled plasma-isotope dilution-mass spectrometry

Ilse Gelaude, Richard Dams UGent, Martin Resano Ezcaray, Frank Vanhaecke UGent and Luc Moens UGent (2002) ANALYTICAL CHEMISTRY. 74(15). p.3833-3842
abstract
This paper reports on the use of solid sampling-electrothermal vaporization-inductively. coupled plasma mass spectrometry (SS-ETV-ICPMS) for the direct and simultaneous determination of methylmercury and inorganic mercury in biological materials. The main advantage of this fast and sensitive method is that no sample preparation is required. In this way, the sample throughput can be considerably increased, problems of contamination and analyte losses are kept to a minimum and, even more important, the original chemical form of the different analyte species in the solid samples is preserved. To achieve this goal, a solid sample is inserted into a graphite furnace of the boat-in-tube type, and is subsequently submitted to an appropriate temperature program, leading to the separate vaporization of methylmercury and inorganic mercury, which are transported into the ICP by means of an argon carrier gas. The separation was accomplished within 75 s. For the quantification of the two peaks, species-unspecific isotope dilution was used. For this purpose, a stable flow of argon loaded with gaseous Hg isotopically enriched in Hg-200 was generated using a permeation tube that was constructed in-house. Its emission rate was determined by collecting the mercury released during a given time interval on a gold-coated silica absorber, after which the amount, collected was released by heating of the absorber and determined by cold vapor atomic absorption spectrometry (CVAAS) and cold vapor atomic fluorescence spectrometry (CVAFS). A reference material from the Canadian National. Research Council (NRC) (TORT-2) was used to assess the accuracy of the,method. For.,the application of the method to samples with diverse mercury contents, the spike/sample ratio can be optimized by varying the emission rate of the permeation tube simply by adapting its temperature. To prove the feasibility of this approach, two reference materials (BCR 463 and DORM-2) with a methylmercury content more than 10 times higher than that of TORT-2 were also analyzed. The detection limits obtained for 1 mg of sample (2 ng g(-1) and 6 ng g(-1) for methylmercury and inorganic mercury, respectively) were found to be sufficiently low for this kind of application and are competitive when compared to other techniques.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ICP-MS, ATOMIC-ABSORPTION SPECTROMETRY, EMISSION-SPECTROMETRY, FLUORESCENCE SPECTROMETRY, SODIUM TETRAETHYLBORATE, LIQUID-CHROMATOGRAPHY, RAPID-DETERMINATION, CALIBRATION METHOD, FISH SAMPLES, SPECIATION
journal title
ANALYTICAL CHEMISTRY
Anal. Chem.
volume
74
issue
15
pages
3833 - 3842
Web of Science type
Article
Web of Science id
000177239200043
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
5.094 (2002)
JCR rank
1/67 (2002)
JCR quartile
1 (2002)
ISSN
0003-2700
DOI
10.1021/ac020060i
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
162470
handle
http://hdl.handle.net/1854/LU-162470
date created
2004-01-14 13:39:00
date last changed
2012-11-14 11:18:54
@article{162470,
  abstract     = {This paper reports on the use of solid sampling-electrothermal vaporization-inductively. coupled plasma mass spectrometry (SS-ETV-ICPMS) for the direct and simultaneous determination of methylmercury and inorganic mercury in biological materials. The main advantage of this fast and sensitive method is that no sample preparation is required. In this way, the sample throughput can be considerably increased, problems of contamination and analyte losses are kept to a minimum and, even more important, the original chemical form of the different analyte species in the solid samples is preserved. To achieve this goal, a solid sample is inserted into a graphite furnace of the boat-in-tube type, and is subsequently submitted to an appropriate temperature program, leading to the separate vaporization of methylmercury and inorganic mercury, which are transported into the ICP by means of an argon carrier gas. The separation was accomplished within 75 s. For the quantification of the two peaks, species-unspecific isotope dilution was used. For this purpose, a stable flow of argon loaded with gaseous Hg isotopically enriched in Hg-200 was generated using a permeation tube that was constructed in-house. Its emission rate was determined by collecting the mercury released during a given time interval on a gold-coated silica absorber, after which the amount, collected was released by heating of the absorber and determined by cold vapor atomic absorption spectrometry (CVAAS) and cold vapor atomic fluorescence spectrometry (CVAFS). A reference material from the Canadian National. Research Council (NRC) (TORT-2) was used to assess the accuracy of the,method. For.,the application of the method to samples with diverse mercury contents, the spike/sample ratio can be optimized by varying the emission rate of the permeation tube simply by adapting its temperature. To prove the feasibility of this approach, two reference materials (BCR 463 and DORM-2) with a methylmercury content more than 10 times higher than that of TORT-2 were also analyzed. The detection limits obtained for 1 mg of sample (2 ng g(-1) and 6 ng g(-1) for methylmercury and inorganic mercury, respectively) were found to be sufficiently low for this kind of application and are competitive when compared to other techniques.},
  author       = {Gelaude, Ilse and Dams, Richard and Resano Ezcaray, Martin and Vanhaecke, Frank and Moens, Luc},
  issn         = {0003-2700},
  journal      = {ANALYTICAL CHEMISTRY},
  keyword      = {ICP-MS,ATOMIC-ABSORPTION SPECTROMETRY,EMISSION-SPECTROMETRY,FLUORESCENCE SPECTROMETRY,SODIUM TETRAETHYLBORATE,LIQUID-CHROMATOGRAPHY,RAPID-DETERMINATION,CALIBRATION METHOD,FISH SAMPLES,SPECIATION},
  language     = {eng},
  number       = {15},
  pages        = {3833--3842},
  title        = {Direct determination of methylmercury and inorganic mercury in biological materials by solid sampling-electrothermal vaporization-inductively coupled plasma-isotope dilution-mass spectrometry},
  url          = {http://dx.doi.org/10.1021/ac020060i},
  volume       = {74},
  year         = {2002},
}

Chicago
Gelaude, Ilse, Richard Dams, Martin Resano Ezcaray, Frank Vanhaecke, and Luc Moens. 2002. “Direct Determination of Methylmercury and Inorganic Mercury in Biological Materials by Solid Sampling-electrothermal Vaporization-inductively Coupled Plasma-isotope Dilution-mass Spectrometry.” Analytical Chemistry 74 (15): 3833–3842.
APA
Gelaude, I., Dams, R., Resano Ezcaray, M., Vanhaecke, F., & Moens, L. (2002). Direct determination of methylmercury and inorganic mercury in biological materials by solid sampling-electrothermal vaporization-inductively coupled plasma-isotope dilution-mass spectrometry. ANALYTICAL CHEMISTRY, 74(15), 3833–3842.
Vancouver
1.
Gelaude I, Dams R, Resano Ezcaray M, Vanhaecke F, Moens L. Direct determination of methylmercury and inorganic mercury in biological materials by solid sampling-electrothermal vaporization-inductively coupled plasma-isotope dilution-mass spectrometry. ANALYTICAL CHEMISTRY. 2002;74(15):3833–42.
MLA
Gelaude, Ilse, Richard Dams, Martin Resano Ezcaray, et al. “Direct Determination of Methylmercury and Inorganic Mercury in Biological Materials by Solid Sampling-electrothermal Vaporization-inductively Coupled Plasma-isotope Dilution-mass Spectrometry.” ANALYTICAL CHEMISTRY 74.15 (2002): 3833–3842. Print.