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Detection and quantification of selenium in proteins by means of gel electrophoresis and electrothermal vaporization ICP-MS

Cyrille C Chéry, H Chassaigne, L Verbeeck, Rita Cornelis UGent, Frank Vanhaecke UGent and Luc Moens UGent (2002) JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY. 17(6). p.576-580
abstract
A method for the separation of selenium-containing proteins and subsequent detection and quantification of selenium was developed. First, the proteins are fractionated by means of sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE); subsequently, the bands of the gel, containing the proteins, are analyzed by electrothermal vaporization (ETV)-ICP-MS to detect and quantify selenium. External standardization with the use of an internal reference (Te) was applied. A detection limit of ca. 40 pg Se per band and a recovery of ca. 98% were obtained. A single measurement is accomplished in less than 4 min and thus a gel lane after a separation of ca. 1.5 h, can be entirely analyzed with ETV-ICP-MS in 3.5 h. If limited to 10 bands, a gel lane is analyzed in 2 h (calibration included). The analysis is directly carried out on the stained gel, without blotting, which makes the analysis even more practical. This method was optimized using the selenoprotein glutathione peroxidase as model. Then, it was applied to the fractionation of proteins from a selenium-yeast candidate reference material. The reconstructed Se electropherograms are presented and compared with the stained gels. The major advantages of this method are the high resolution of the protein fractionation and the straightforward quantification of selenium.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SE, ATOMIC-ABSORPTION SPECTROMETRY, PLASMA-MASS SPECTROMETRY, BIOLOGICAL-MATERIALS, ETV, MECHANISM, PALLADIUM
journal title
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
J. Anal. At. Spectrom.
volume
17
issue
6
pages
576 - 580
Web of Science type
Article
Web of Science id
000176052400003
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
4.25 (2002)
JCR rank
4/67 (2002)
JCR quartile
1 (2002)
ISSN
0267-9477
DOI
10.1039/b201979p
language
English
UGent publication?
yes
classification
A1
id
162427
handle
http://hdl.handle.net/1854/LU-162427
date created
2004-01-14 13:39:00
date last changed
2012-11-14 10:57:28
@article{162427,
  abstract     = {A method for the separation of selenium-containing proteins and subsequent detection and quantification of selenium was developed. First, the proteins are fractionated by means of sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE); subsequently, the bands of the gel, containing the proteins, are analyzed by electrothermal vaporization (ETV)-ICP-MS to detect and quantify selenium. External standardization with the use of an internal reference (Te) was applied. A detection limit of ca. 40 pg Se per band and a recovery of ca. 98\% were obtained. A single measurement is accomplished in less than 4 min and thus a gel lane after a separation of ca. 1.5 h, can be entirely analyzed with ETV-ICP-MS in 3.5 h. If limited to 10 bands, a gel lane is analyzed in 2 h (calibration included). The analysis is directly carried out on the stained gel, without blotting, which makes the analysis even more practical. This method was optimized using the selenoprotein glutathione peroxidase as model. Then, it was applied to the fractionation of proteins from a selenium-yeast candidate reference material. The reconstructed Se electropherograms are presented and compared with the stained gels. The major advantages of this method are the high resolution of the protein fractionation and the straightforward quantification of selenium.},
  author       = {Ch{\'e}ry, Cyrille C and Chassaigne, H and Verbeeck, L and Cornelis, Rita and Vanhaecke, Frank and Moens, Luc},
  issn         = {0267-9477},
  journal      = {JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY},
  keyword      = {SE,ATOMIC-ABSORPTION SPECTROMETRY,PLASMA-MASS SPECTROMETRY,BIOLOGICAL-MATERIALS,ETV,MECHANISM,PALLADIUM},
  language     = {eng},
  number       = {6},
  pages        = {576--580},
  title        = {Detection and quantification of selenium in proteins by means of gel electrophoresis and electrothermal vaporization ICP-MS},
  url          = {http://dx.doi.org/10.1039/b201979p},
  volume       = {17},
  year         = {2002},
}

Chicago
Chéry, Cyrille C, H Chassaigne, L Verbeeck, Rita Cornelis, Frank Vanhaecke, and Luc Moens. 2002. “Detection and Quantification of Selenium in Proteins by Means of Gel Electrophoresis and Electrothermal Vaporization ICP-MS.” Journal of Analytical Atomic Spectrometry 17 (6): 576–580.
APA
Chéry, C. C., Chassaigne, H., Verbeeck, L., Cornelis, R., Vanhaecke, F., & Moens, L. (2002). Detection and quantification of selenium in proteins by means of gel electrophoresis and electrothermal vaporization ICP-MS. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 17(6), 576–580.
Vancouver
1.
Chéry CC, Chassaigne H, Verbeeck L, Cornelis R, Vanhaecke F, Moens L. Detection and quantification of selenium in proteins by means of gel electrophoresis and electrothermal vaporization ICP-MS. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY. 2002;17(6):576–80.
MLA
Chéry, Cyrille C, H Chassaigne, L Verbeeck, et al. “Detection and Quantification of Selenium in Proteins by Means of Gel Electrophoresis and Electrothermal Vaporization ICP-MS.” JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY 17.6 (2002): 576–580. Print.