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An improved method for whole-mount in situ hybridization of Heterodera schachtii juveniles

(2002) PARASITOLOGY RESEARCH. 88(8). p.731-733
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Abstract
An optimized protocol is presented to visualize gene expression in the sedentary beet cyst nematode, Heterodera schachtii, by whole-mount in situ hybridization. Two different probes were used for genes with known expression pattern in other nematodes. Vacuum infiltration of the fixative significantly increased its efficiency and resulted in a nicely preserved morphology. Additional modifications were introduced to simplify and standardize the process.
Keywords
NEMATODE, GLYCINES, BINDING, PROBES, RNA, GENES

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Citation

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Chicago
Vanholme, Bartel, Jan De Meutter, Tom Tytgat, Greetje Gheysen, Isabelle Vanhoutte, and Godelieve Gheysen. 2002. “An Improved Method for Whole-mount in Situ Hybridization of Heterodera Schachtii Juveniles.” Parasitology Research 88 (8): 731–733.
APA
Vanholme, B., De Meutter, J., Tytgat, T., Gheysen, G., Vanhoutte, I., & Gheysen, G. (2002). An improved method for whole-mount in situ hybridization of Heterodera schachtii juveniles. PARASITOLOGY RESEARCH, 88(8), 731–733.
Vancouver
1.
Vanholme B, De Meutter J, Tytgat T, Gheysen G, Vanhoutte I, Gheysen G. An improved method for whole-mount in situ hybridization of Heterodera schachtii juveniles. PARASITOLOGY RESEARCH. 2002;88(8):731–3.
MLA
Vanholme, Bartel, Jan De Meutter, Tom Tytgat, et al. “An Improved Method for Whole-mount in Situ Hybridization of Heterodera Schachtii Juveniles.” PARASITOLOGY RESEARCH 88.8 (2002): 731–733. Print.
@article{158486,
  abstract     = {An optimized protocol is presented to visualize gene expression in the sedentary beet cyst nematode, Heterodera schachtii, by whole-mount in situ hybridization. Two different probes were used for genes with known expression pattern in other nematodes. Vacuum infiltration of the fixative significantly increased its efficiency and resulted in a nicely preserved morphology. Additional modifications were introduced to simplify and standardize the process.},
  author       = {Vanholme, Bartel and De Meutter, Jan and Tytgat, Tom and Gheysen, Greetje and Vanhoutte, Isabelle and Gheysen, Godelieve},
  issn         = {0932-0113},
  journal      = {PARASITOLOGY RESEARCH},
  keyword      = {NEMATODE,GLYCINES,BINDING,PROBES,RNA,GENES},
  language     = {eng},
  number       = {8},
  pages        = {731--733},
  title        = {An improved method for whole-mount in situ hybridization of Heterodera schachtii juveniles},
  url          = {http://dx.doi.org/10.1007/s00436-002-0661-0},
  volume       = {88},
  year         = {2002},
}

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