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An improved method for whole-mount in situ hybridization of Heterodera schachtii juveniles

Bartel Vanholme UGent, Jan De Meutter UGent, Tom Tytgat UGent, Greetje Gheysen, Isabelle Vanhoutte UGent and Godelieve Gheysen UGent (2002) PARASITOLOGY RESEARCH. 88(8). p.731-733
abstract
An optimized protocol is presented to visualize gene expression in the sedentary beet cyst nematode, Heterodera schachtii, by whole-mount in situ hybridization. Two different probes were used for genes with known expression pattern in other nematodes. Vacuum infiltration of the fixative significantly increased its efficiency and resulted in a nicely preserved morphology. Additional modifications were introduced to simplify and standardize the process.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
NEMATODE, GLYCINES, BINDING, PROBES, RNA, GENES
journal title
PARASITOLOGY RESEARCH
Parasitol. Res.
volume
88
issue
8
pages
731 - 733
Web of Science type
Article
Web of Science id
000177204700003
JCR category
PARASITOLOGY
JCR impact factor
1.046 (2002)
JCR rank
12/22 (2002)
JCR quartile
3 (2002)
ISSN
0932-0113
DOI
10.1007/s00436-002-0661-0
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
158486
handle
http://hdl.handle.net/1854/LU-158486
date created
2004-01-14 13:39:00
date last changed
2016-12-19 15:38:41
@article{158486,
  abstract     = {An optimized protocol is presented to visualize gene expression in the sedentary beet cyst nematode, Heterodera schachtii, by whole-mount in situ hybridization. Two different probes were used for genes with known expression pattern in other nematodes. Vacuum infiltration of the fixative significantly increased its efficiency and resulted in a nicely preserved morphology. Additional modifications were introduced to simplify and standardize the process.},
  author       = {Vanholme, Bartel and De Meutter, Jan and Tytgat, Tom and Gheysen, Greetje and Vanhoutte, Isabelle and Gheysen, Godelieve},
  issn         = {0932-0113},
  journal      = {PARASITOLOGY RESEARCH},
  keyword      = {NEMATODE,GLYCINES,BINDING,PROBES,RNA,GENES},
  language     = {eng},
  number       = {8},
  pages        = {731--733},
  title        = {An improved method for whole-mount in situ hybridization of Heterodera schachtii juveniles},
  url          = {http://dx.doi.org/10.1007/s00436-002-0661-0},
  volume       = {88},
  year         = {2002},
}

Chicago
Vanholme, Bartel, Jan De Meutter, Tom Tytgat, Greetje Gheysen, Isabelle Vanhoutte, and Godelieve Gheysen. 2002. “An Improved Method for Whole-mount in Situ Hybridization of Heterodera Schachtii Juveniles.” Parasitology Research 88 (8): 731–733.
APA
Vanholme, B., De Meutter, J., Tytgat, T., Gheysen, G., Vanhoutte, I., & Gheysen, G. (2002). An improved method for whole-mount in situ hybridization of Heterodera schachtii juveniles. PARASITOLOGY RESEARCH, 88(8), 731–733.
Vancouver
1.
Vanholme B, De Meutter J, Tytgat T, Gheysen G, Vanhoutte I, Gheysen G. An improved method for whole-mount in situ hybridization of Heterodera schachtii juveniles. PARASITOLOGY RESEARCH. 2002;88(8):731–3.
MLA
Vanholme, Bartel, Jan De Meutter, Tom Tytgat, et al. “An Improved Method for Whole-mount in Situ Hybridization of Heterodera Schachtii Juveniles.” PARASITOLOGY RESEARCH 88.8 (2002): 731–733. Print.