Dual-color fluorescence fluctuation spectroscopy to study the complexation between poly-l-lysine and oligonucleotides
- Author
- Bart Lucas (UGent) , Elsa Van Rompaey, Stefaan De Smedt (UGent) , Jo Demeester (UGent) and Patric Van Oostveldt (UGent)
- Organization
- Abstract
- In this study, dual-color fluorescence fluctuation spectroscopy (FFS) was explored to characterize the association of oligonucleotides (ONs) to cationic polymers. The results from dual-color FFS, in which both the ONs and the cationic polymers were fluorescently labeled, were compared with data obtained from single-color FFS in which only the ONs or the cationic polymers were fluorescently marked. As a model, the association of negatively charged 20-mer ONs to positively charged poly-L-lysine (pLL) was studied. The binding of rhodamine green-labeled ONs (RhGr-ONs) to nonlabeled pLL could be clearly observed in the fluorescence fluctuation profiles. Especially, highly intense fluorescence peaks appeared in the fluorescence fluctuations. A highly intense fluorescence peak was considered to originate from one complex in which a number of RhGr-ONs were present. Complexing nonlabeled ONs to rhodamine green-labeled pLL (RhGr-pLL) also resulted in highly intense fluorescence peaks, indicating not only that the complexes consisted of a number of ONs but also that numerous RhGr-pLL chains were present. Upon complexation of red-labeled pLL (Cy5-pLL) to green-labeled ONs (RhGr-ONs), highly intense fluorescence peaks occurred simultaneously in the red and green detector. These data proved the multimolecular composition of the polyplexes and agreed with the observations from single-color FFS.
- Keywords
- CELLS, DNA, GENE DELIVERY SYSTEMS, CROSS-CORRELATION SPECTROSCOPY
Downloads
-
(...).pdf
- full text
- |
- UGent only
- |
- |
- 310.44 KB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-158435
- MLA
- Lucas, Bart, et al. “Dual-Color Fluorescence Fluctuation Spectroscopy to Study the Complexation between Poly-l-Lysine and Oligonucleotides.” MACROMOLECULES, vol. 35, no. 21, 2002, pp. 8152–60, doi:10.1021/ma0202383.
- APA
- Lucas, B., Van Rompaey, E., De Smedt, S., Demeester, J., & Van Oostveldt, P. (2002). Dual-color fluorescence fluctuation spectroscopy to study the complexation between poly-l-lysine and oligonucleotides. MACROMOLECULES, 35(21), 8152–8160. https://doi.org/10.1021/ma0202383
- Chicago author-date
- Lucas, Bart, Elsa Van Rompaey, Stefaan De Smedt, Jo Demeester, and Patric Van Oostveldt. 2002. “Dual-Color Fluorescence Fluctuation Spectroscopy to Study the Complexation between Poly-l-Lysine and Oligonucleotides.” MACROMOLECULES 35 (21): 8152–60. https://doi.org/10.1021/ma0202383.
- Chicago author-date (all authors)
- Lucas, Bart, Elsa Van Rompaey, Stefaan De Smedt, Jo Demeester, and Patric Van Oostveldt. 2002. “Dual-Color Fluorescence Fluctuation Spectroscopy to Study the Complexation between Poly-l-Lysine and Oligonucleotides.” MACROMOLECULES 35 (21): 8152–8160. doi:10.1021/ma0202383.
- Vancouver
- 1.Lucas B, Van Rompaey E, De Smedt S, Demeester J, Van Oostveldt P. Dual-color fluorescence fluctuation spectroscopy to study the complexation between poly-l-lysine and oligonucleotides. MACROMOLECULES. 2002;35(21):8152–60.
- IEEE
- [1]B. Lucas, E. Van Rompaey, S. De Smedt, J. Demeester, and P. Van Oostveldt, “Dual-color fluorescence fluctuation spectroscopy to study the complexation between poly-l-lysine and oligonucleotides,” MACROMOLECULES, vol. 35, no. 21, pp. 8152–8160, 2002.
@article{158435, abstract = {{In this study, dual-color fluorescence fluctuation spectroscopy (FFS) was explored to characterize the association of oligonucleotides (ONs) to cationic polymers. The results from dual-color FFS, in which both the ONs and the cationic polymers were fluorescently labeled, were compared with data obtained from single-color FFS in which only the ONs or the cationic polymers were fluorescently marked. As a model, the association of negatively charged 20-mer ONs to positively charged poly-L-lysine (pLL) was studied. The binding of rhodamine green-labeled ONs (RhGr-ONs) to nonlabeled pLL could be clearly observed in the fluorescence fluctuation profiles. Especially, highly intense fluorescence peaks appeared in the fluorescence fluctuations. A highly intense fluorescence peak was considered to originate from one complex in which a number of RhGr-ONs were present. Complexing nonlabeled ONs to rhodamine green-labeled pLL (RhGr-pLL) also resulted in highly intense fluorescence peaks, indicating not only that the complexes consisted of a number of ONs but also that numerous RhGr-pLL chains were present. Upon complexation of red-labeled pLL (Cy5-pLL) to green-labeled ONs (RhGr-ONs), highly intense fluorescence peaks occurred simultaneously in the red and green detector. These data proved the multimolecular composition of the polyplexes and agreed with the observations from single-color FFS.}}, author = {{Lucas, Bart and Van Rompaey, Elsa and De Smedt, Stefaan and Demeester, Jo and Van Oostveldt, Patric}}, issn = {{0024-9297}}, journal = {{MACROMOLECULES}}, keywords = {{CELLS,DNA,GENE DELIVERY SYSTEMS,CROSS-CORRELATION SPECTROSCOPY}}, language = {{eng}}, number = {{21}}, pages = {{8152--8160}}, title = {{Dual-color fluorescence fluctuation spectroscopy to study the complexation between poly-l-lysine and oligonucleotides}}, url = {{http://doi.org/10.1021/ma0202383}}, volume = {{35}}, year = {{2002}}, }
- Altmetric
- View in Altmetric
- Web of Science
- Times cited: