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Dual-color fluorescence fluctuation spectroscopy to study the complexation between poly-l-lysine and oligonucleotides

Bart Lucas UGent, Elsa Van Rompaey, Stefaan De Smedt UGent, Jo Demeester UGent and Patric Van Oostveldt UGent (2002) MACROMOLECULES. 35(21). p.8152-8160
abstract
In this study, dual-color fluorescence fluctuation spectroscopy (FFS) was explored to characterize the association of oligonucleotides (ONs) to cationic polymers. The results from dual-color FFS, in which both the ONs and the cationic polymers were fluorescently labeled, were compared with data obtained from single-color FFS in which only the ONs or the cationic polymers were fluorescently marked. As a model, the association of negatively charged 20-mer ONs to positively charged poly-L-lysine (pLL) was studied. The binding of rhodamine green-labeled ONs (RhGr-ONs) to nonlabeled pLL could be clearly observed in the fluorescence fluctuation profiles. Especially, highly intense fluorescence peaks appeared in the fluorescence fluctuations. A highly intense fluorescence peak was considered to originate from one complex in which a number of RhGr-ONs were present. Complexing nonlabeled ONs to rhodamine green-labeled pLL (RhGr-pLL) also resulted in highly intense fluorescence peaks, indicating not only that the complexes consisted of a number of ONs but also that numerous RhGr-pLL chains were present. Upon complexation of red-labeled pLL (Cy5-pLL) to green-labeled ONs (RhGr-ONs), highly intense fluorescence peaks occurred simultaneously in the red and green detector. These data proved the multimolecular composition of the polyplexes and agreed with the observations from single-color FFS.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CELLS, DNA, GENE DELIVERY SYSTEMS, CROSS-CORRELATION SPECTROSCOPY
journal title
MACROMOLECULES
Macromolecules
volume
35
issue
21
pages
8152 - 8160
Web of Science type
Article
Web of Science id
000178444400041
JCR category
POLYMER SCIENCE
JCR impact factor
3.751 (2002)
JCR rank
3/73 (2002)
JCR quartile
1 (2002)
ISSN
0024-9297
DOI
10.1021/ma0202383
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
158435
handle
http://hdl.handle.net/1854/LU-158435
date created
2004-01-14 13:39:00
date last changed
2010-08-18 14:55:10
@article{158435,
  abstract     = {In this study, dual-color fluorescence fluctuation spectroscopy (FFS) was explored to characterize the association of oligonucleotides (ONs) to cationic polymers. The results from dual-color FFS, in which both the ONs and the cationic polymers were fluorescently labeled, were compared with data obtained from single-color FFS in which only the ONs or the cationic polymers were fluorescently marked. As a model, the association of negatively charged 20-mer ONs to positively charged poly-L-lysine (pLL) was studied. The binding of rhodamine green-labeled ONs (RhGr-ONs) to nonlabeled pLL could be clearly observed in the fluorescence fluctuation profiles. Especially, highly intense fluorescence peaks appeared in the fluorescence fluctuations. A highly intense fluorescence peak was considered to originate from one complex in which a number of RhGr-ONs were present. Complexing nonlabeled ONs to rhodamine green-labeled pLL (RhGr-pLL) also resulted in highly intense fluorescence peaks, indicating not only that the complexes consisted of a number of ONs but also that numerous RhGr-pLL chains were present. Upon complexation of red-labeled pLL (Cy5-pLL) to green-labeled ONs (RhGr-ONs), highly intense fluorescence peaks occurred simultaneously in the red and green detector. These data proved the multimolecular composition of the polyplexes and agreed with the observations from single-color FFS.},
  author       = {Lucas, Bart and Van Rompaey, Elsa and De Smedt, Stefaan and Demeester, Jo and Van Oostveldt, Patric},
  issn         = {0024-9297},
  journal      = {MACROMOLECULES},
  keyword      = {CELLS,DNA,GENE DELIVERY SYSTEMS,CROSS-CORRELATION SPECTROSCOPY},
  language     = {eng},
  number       = {21},
  pages        = {8152--8160},
  title        = {Dual-color fluorescence fluctuation spectroscopy to study the complexation between poly-l-lysine and oligonucleotides},
  url          = {http://dx.doi.org/10.1021/ma0202383},
  volume       = {35},
  year         = {2002},
}

Chicago
Lucas, Bart, Elsa Van Rompaey, Stefaan De Smedt, Jo Demeester, and Patric Van Oostveldt. 2002. “Dual-color Fluorescence Fluctuation Spectroscopy to Study the Complexation Between Poly-l-lysine and Oligonucleotides.” Macromolecules 35 (21): 8152–8160.
APA
Lucas, B., Van Rompaey, E., De Smedt, S., Demeester, J., & Van Oostveldt, P. (2002). Dual-color fluorescence fluctuation spectroscopy to study the complexation between poly-l-lysine and oligonucleotides. MACROMOLECULES, 35(21), 8152–8160.
Vancouver
1.
Lucas B, Van Rompaey E, De Smedt S, Demeester J, Van Oostveldt P. Dual-color fluorescence fluctuation spectroscopy to study the complexation between poly-l-lysine and oligonucleotides. MACROMOLECULES. 2002;35(21):8152–60.
MLA
Lucas, Bart, Elsa Van Rompaey, Stefaan De Smedt, et al. “Dual-color Fluorescence Fluctuation Spectroscopy to Study the Complexation Between Poly-l-lysine and Oligonucleotides.” MACROMOLECULES 35.21 (2002): 8152–8160. Print.