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Assessment of the estrogenic activity of flue gases from burning processes by means of the yeast based human estrogen receptor (hER) bioassay

Waweru Muthumbi, Patrick De Boever, Isabel D'Haese, W D'HOOGE, Eva Top, Jan Pieters (UGent) , Frank Comhaire and Willy Verstraete (UGent)
(2002) ENVIRONMENTAL TECHNOLOGY. 23(3). p.287-291
Author
Organization
Abstract
Combustion processes are known to produce organic micro-pollutants in the flue gas at concentrations ranging over several orders of magnitude. Some organic micro-pollutants are suspected of being pseudo-estrogens and as such they can affect the public health. In this study, the possible application of the yeast based human estrogen receptor (hER) bioassay to screen flue gas streams for the presence of estrogenic active micro-pollutants was explored. Specifically, the protocol was modified to allow the detection and quantification of the potential estrogenic active non-polar organic micro-pollutants contained in the flue gas matrix. The modified assay was calibrated using a model estrogenic compound (17-alpha-ethinylestradiol (EE2)) dissolved in methylene chloride at concentrations ranging from 3 ng l(-1) to 3000 ng l(-1). The effective concentration to elucidate a 50% response (EC50) was 87 ngl(-1) of EE2 equivalent dissolved in methylene chloride. Samples of methylene chloride used to trap non-polar micro-pollutants in flue gas from combustion of pine wood were found to clearly register estrogenic activity by the bioassay under certain conditions. The combustion tests were performed with pinewood alone and with pine wood in the presence of both Copper-naphthenate and copper(II)chloride at 600degreesC and 1000degreesC. These conditions must be considered as experimental rather than practical. Overall, the results suggest that, by means of this modified assay, it is possible and warranted to screen systematically for estrogens in flue gas combustion processes.

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MLA
Muthumbi, Waweru, et al. “Assessment of the Estrogenic Activity of Flue Gases from Burning Processes by Means of the Yeast Based Human Estrogen Receptor (HER) Bioassay.” ENVIRONMENTAL TECHNOLOGY, vol. 23, no. 3, 2002, pp. 287–91, doi:10.1080/09593332508618415.
APA
Muthumbi, W., De Boever, P., D’Haese, I., D’HOOGE, W., Top, E., Pieters, J., … Verstraete, W. (2002). Assessment of the estrogenic activity of flue gases from burning processes by means of the yeast based human estrogen receptor (hER) bioassay. ENVIRONMENTAL TECHNOLOGY, 23(3), 287–291. https://doi.org/10.1080/09593332508618415
Chicago author-date
Muthumbi, Waweru, Patrick De Boever, Isabel D’Haese, W D’HOOGE, Eva Top, Jan Pieters, Frank Comhaire, and Willy Verstraete. 2002. “Assessment of the Estrogenic Activity of Flue Gases from Burning Processes by Means of the Yeast Based Human Estrogen Receptor (HER) Bioassay.” ENVIRONMENTAL TECHNOLOGY 23 (3): 287–91. https://doi.org/10.1080/09593332508618415.
Chicago author-date (all authors)
Muthumbi, Waweru, Patrick De Boever, Isabel D’Haese, W D’HOOGE, Eva Top, Jan Pieters, Frank Comhaire, and Willy Verstraete. 2002. “Assessment of the Estrogenic Activity of Flue Gases from Burning Processes by Means of the Yeast Based Human Estrogen Receptor (HER) Bioassay.” ENVIRONMENTAL TECHNOLOGY 23 (3): 287–291. doi:10.1080/09593332508618415.
Vancouver
1.
Muthumbi W, De Boever P, D’Haese I, D’HOOGE W, Top E, Pieters J, et al. Assessment of the estrogenic activity of flue gases from burning processes by means of the yeast based human estrogen receptor (hER) bioassay. ENVIRONMENTAL TECHNOLOGY. 2002;23(3):287–91.
IEEE
[1]
W. Muthumbi et al., “Assessment of the estrogenic activity of flue gases from burning processes by means of the yeast based human estrogen receptor (hER) bioassay,” ENVIRONMENTAL TECHNOLOGY, vol. 23, no. 3, pp. 287–291, 2002.
@article{157400,
  abstract     = {{Combustion processes are known to produce organic micro-pollutants in the flue gas at concentrations ranging over several orders of magnitude. Some organic micro-pollutants are suspected of being pseudo-estrogens and as such they can affect the public health. In this study, the possible application of the yeast based human estrogen receptor (hER) bioassay to screen flue gas streams for the presence of estrogenic active micro-pollutants was explored. Specifically, the protocol was modified to allow the detection and quantification of the potential estrogenic active non-polar organic micro-pollutants contained in the flue gas matrix. The modified assay was calibrated using a model estrogenic compound (17-alpha-ethinylestradiol (EE2)) dissolved in methylene chloride at concentrations ranging from 3 ng l(-1) to 3000 ng l(-1). The effective concentration to elucidate a 50% response (EC50) was 87 ngl(-1) of EE2 equivalent dissolved in methylene chloride. Samples of methylene chloride used to trap non-polar micro-pollutants in flue gas from combustion of pine wood were found to clearly register estrogenic activity by the bioassay under certain conditions. The combustion tests were performed with pinewood alone and with pine wood in the presence of both Copper-naphthenate and copper(II)chloride at 600degreesC and 1000degreesC. These conditions must be considered as experimental rather than practical. Overall, the results suggest that, by means of this modified assay, it is possible and warranted to screen systematically for estrogens in flue gas combustion processes.}},
  author       = {{Muthumbi, Waweru and De Boever, Patrick and D'Haese, Isabel and D'HOOGE, W and Top, Eva and Pieters, Jan and Comhaire, Frank and Verstraete, Willy}},
  issn         = {{0959-3330}},
  journal      = {{ENVIRONMENTAL TECHNOLOGY}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{287--291}},
  title        = {{Assessment of the estrogenic activity of flue gases from burning processes by means of the yeast based human estrogen receptor (hER) bioassay}},
  url          = {{http://doi.org/10.1080/09593332508618415}},
  volume       = {{23}},
  year         = {{2002}},
}
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