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Assessment of the estrogenic activity of flue gases from burning processes by means of the yeast based human estrogen receptor (hER) bioassay

Waweru Muthumbi, Patrick De Boever, Isabel D'Haese, W D'HOOGE, Eva Top, Jan Pieters UGent, Frank Comhaire UGent and Willy Verstraete UGent (2002) ENVIRONMENTAL TECHNOLOGY. 23(3). p.287-291
abstract
Combustion processes are known to produce organic micro-pollutants in the flue gas at concentrations ranging over several orders of magnitude. Some organic micro-pollutants are suspected of being pseudo-estrogens and as such they can affect the public health. In this study, the possible application of the yeast based human estrogen receptor (hER) bioassay to screen flue gas streams for the presence of estrogenic active micro-pollutants was explored. Specifically, the protocol was modified to allow the detection and quantification of the potential estrogenic active non-polar organic micro-pollutants contained in the flue gas matrix. The modified assay was calibrated using a model estrogenic compound (17-alpha-ethinylestradiol (EE2)) dissolved in methylene chloride at concentrations ranging from 3 ng l(-1) to 3000 ng l(-1). The effective concentration to elucidate a 50% response (EC50) was 87 ngl(-1) of EE2 equivalent dissolved in methylene chloride. Samples of methylene chloride used to trap non-polar micro-pollutants in flue gas from combustion of pine wood were found to clearly register estrogenic activity by the bioassay under certain conditions. The combustion tests were performed with pinewood alone and with pine wood in the presence of both Copper-naphthenate and copper(II)chloride at 600degreesC and 1000degreesC. These conditions must be considered as experimental rather than practical. Overall, the results suggest that, by means of this modified assay, it is possible and warranted to screen systematically for estrogens in flue gas combustion processes.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
ENVIRONMENTAL TECHNOLOGY
Environ. Technol.
volume
23
issue
3
pages
287-291 pages
Web of Science type
Article
Web of Science id
000175613800005
JCR category
ENVIRONMENTAL SCIENCES
JCR impact factor
0.616 (2002)
JCR rank
88/130 (2002)
JCR quartile
3 (2002)
ISSN
0959-3330
DOI
10.1080/09593332508618415
language
English
UGent publication?
yes
classification
A1
id
157400
handle
http://hdl.handle.net/1854/LU-157400
date created
2005-08-23 15:18:00
date last changed
2010-02-25 10:44:07
@article{157400,
  abstract     = {Combustion processes are known to produce organic micro-pollutants in the flue gas at concentrations ranging over several orders of magnitude. Some organic micro-pollutants are suspected of being pseudo-estrogens and as such they can affect the public health. In this study, the possible application of the yeast based human estrogen receptor (hER) bioassay to screen flue gas streams for the presence of estrogenic active micro-pollutants was explored. Specifically, the protocol was modified to allow the detection and quantification of the potential estrogenic active non-polar organic micro-pollutants contained in the flue gas matrix. The modified assay was calibrated using a model estrogenic compound (17-alpha-ethinylestradiol (EE2)) dissolved in methylene chloride at concentrations ranging from 3 ng l(-1) to 3000 ng l(-1). The effective concentration to elucidate a 50\% response (EC50) was 87 ngl(-1) of EE2 equivalent dissolved in methylene chloride. Samples of methylene chloride used to trap non-polar micro-pollutants in flue gas from combustion of pine wood were found to clearly register estrogenic activity by the bioassay under certain conditions. The combustion tests were performed with pinewood alone and with pine wood in the presence of both Copper-naphthenate and copper(II)chloride at 600degreesC and 1000degreesC. These conditions must be considered as experimental rather than practical. Overall, the results suggest that, by means of this modified assay, it is possible and warranted to screen systematically for estrogens in flue gas combustion processes.},
  author       = {Muthumbi, Waweru and De Boever, Patrick and D'Haese, Isabel and D'HOOGE, W and Top, Eva and Pieters, Jan and Comhaire, Frank and Verstraete, Willy},
  issn         = {0959-3330},
  journal      = {ENVIRONMENTAL TECHNOLOGY},
  language     = {eng},
  number       = {3},
  pages        = {287--291},
  title        = {Assessment of the estrogenic activity of flue gases from burning processes by means of the yeast based human estrogen receptor (hER) bioassay},
  url          = {http://dx.doi.org/10.1080/09593332508618415},
  volume       = {23},
  year         = {2002},
}

Chicago
Muthumbi, Waweru, Patrick De Boever, Isabel D’Haese, W D’HOOGE, Eva Top, Jan Pieters, Frank Comhaire, and Willy Verstraete. 2002. “Assessment of the Estrogenic Activity of Flue Gases from Burning Processes by Means of the Yeast Based Human Estrogen Receptor (hER) Bioassay.” Environmental Technology 23 (3): 287–291.
APA
Muthumbi, W., De Boever, P., D’Haese, I., D’HOOGE, W., Top, E., Pieters, J., Comhaire, F., et al. (2002). Assessment of the estrogenic activity of flue gases from burning processes by means of the yeast based human estrogen receptor (hER) bioassay. ENVIRONMENTAL TECHNOLOGY, 23(3), 287–291.
Vancouver
1.
Muthumbi W, De Boever P, D’Haese I, D’HOOGE W, Top E, Pieters J, et al. Assessment of the estrogenic activity of flue gases from burning processes by means of the yeast based human estrogen receptor (hER) bioassay. ENVIRONMENTAL TECHNOLOGY. 2002;23(3):287–91.
MLA
Muthumbi, Waweru, Patrick De Boever, Isabel D’Haese, et al. “Assessment of the Estrogenic Activity of Flue Gases from Burning Processes by Means of the Yeast Based Human Estrogen Receptor (hER) Bioassay.” ENVIRONMENTAL TECHNOLOGY 23.3 (2002): 287–291. Print.