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Bioaugmenting bioreactors for the continuous removal of 3-chloroaniline by slow release approach

Nico Boon UGent, Leen De Gelder UGent, Hanne Lievens, Steven D Siciliano, Eva M Top and Willy Verstraete UGent (2002) ENVIRONMENTAL SCIENCE AND TECHNOLOGY. 36(21). p.4698-4704
abstract
The survival and activity of microbial degradative inoculants in bioreactors is critical to obtain successful biodegradation of non- or slowly degradable pollutants. Achieving this in industrial wastewater reactors is technically challenging. We evaluated a strategy to obtain complete and stable bioaugmentation of activated sludge, which is used to treat a 3-chloroaniline (3-CA) contaminated wastewater in a lab-scale semi-continuous activated sludge system. A 3-CA metabolizing bacterium, Comamonas testosteroni strain 12, was mixed with molten agar and encapsulated in 4 mm diameter open-ended silicone tubes of 3 cm long. The tubes containing the immobilized bacteria represented about 1% of the volume of the mixed liquor. The bioaugmentation activity of a reactor containing the immobilized cells was compared with a reactor with suspended I2gfp cells. From day 25-30 after inoculation, the reactor with only suspended cells failed to completely degrade 3-CA because of a decrease in metabolic activity. In the reactors with immobilized cells, however, MA continued to be removed. A mass balance indicated that ca. 10% of the degradation activity was due to the immobilized cells. Slow release of the growing embedded cells from the agar into the activated sludge medium, resulting in a higher number of active 3-CA-degrading 12 cells, was responsible for ca. 90% of the degradation. Our results demonstrate that this simple immobilization procedure was effective to maintain a 3-CA-degrading population within the activated sludge community.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ACTIVATED-SLUDGE UNIT, DEGRADING BACTERIA, BED REACTOR, PSEUDOMONAS, DEGRADATION, PHENOL, CELLS, WASTE-WATER, SURVIVAL, ANILINE
journal title
ENVIRONMENTAL SCIENCE AND TECHNOLOGY
Environ. Sci. Technol.
volume
36
issue
21
pages
4698-4704 pages
Web of Science type
Article
Web of Science id
000179002700048
JCR category
ENGINEERING, ENVIRONMENTAL
JCR impact factor
3.123 (2002)
JCR rank
1/37 (2002)
JCR quartile
1 (2002)
ISSN
0013-936X
DOI
10.1021/es020076q
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
157356
handle
http://hdl.handle.net/1854/LU-157356
date created
2004-01-14 13:39:00
date last changed
2016-12-19 15:38:54
@article{157356,
  abstract     = {The survival and activity of microbial degradative inoculants in bioreactors is critical to obtain successful biodegradation of non- or slowly degradable pollutants. Achieving this in industrial wastewater reactors is technically challenging. We evaluated a strategy to obtain complete and stable bioaugmentation of activated sludge, which is used to treat a 3-chloroaniline (3-CA) contaminated wastewater in a lab-scale semi-continuous activated sludge system. A 3-CA metabolizing bacterium, Comamonas testosteroni strain 12, was mixed with molten agar and encapsulated in 4 mm diameter open-ended silicone tubes of 3 cm long. The tubes containing the immobilized bacteria represented about 1\% of the volume of the mixed liquor. The bioaugmentation activity of a reactor containing the immobilized cells was compared with a reactor with suspended I2gfp cells. From day 25-30 after inoculation, the reactor with only suspended cells failed to completely degrade 3-CA because of a decrease in metabolic activity. In the reactors with immobilized cells, however, MA continued to be removed. A mass balance indicated that ca. 10\% of the degradation activity was due to the immobilized cells. Slow release of the growing embedded cells from the agar into the activated sludge medium, resulting in a higher number of active 3-CA-degrading 12 cells, was responsible for ca. 90\% of the degradation. Our results demonstrate that this simple immobilization procedure was effective to maintain a 3-CA-degrading population within the activated sludge community.},
  author       = {Boon, Nico and De Gelder, Leen and Lievens, Hanne and Siciliano, Steven D and Top, Eva M and Verstraete, Willy},
  issn         = {0013-936X},
  journal      = {ENVIRONMENTAL SCIENCE AND TECHNOLOGY},
  keyword      = {ACTIVATED-SLUDGE UNIT,DEGRADING BACTERIA,BED REACTOR,PSEUDOMONAS,DEGRADATION,PHENOL,CELLS,WASTE-WATER,SURVIVAL,ANILINE},
  language     = {eng},
  number       = {21},
  pages        = {4698--4704},
  title        = {Bioaugmenting bioreactors for the continuous removal of 3-chloroaniline by slow release approach},
  url          = {http://dx.doi.org/10.1021/es020076q},
  volume       = {36},
  year         = {2002},
}

Chicago
Boon, Nico, Leen De Gelder, Hanne Lievens, Steven D Siciliano, Eva M Top, and Willy Verstraete. 2002. “Bioaugmenting Bioreactors for the Continuous Removal of 3-chloroaniline by Slow Release Approach.” Environmental Science and Technology 36 (21): 4698–4704.
APA
Boon, N., De Gelder, L., Lievens, H., Siciliano, S. D., Top, E. M., & Verstraete, W. (2002). Bioaugmenting bioreactors for the continuous removal of 3-chloroaniline by slow release approach. ENVIRONMENTAL SCIENCE AND TECHNOLOGY, 36(21), 4698–4704.
Vancouver
1.
Boon N, De Gelder L, Lievens H, Siciliano SD, Top EM, Verstraete W. Bioaugmenting bioreactors for the continuous removal of 3-chloroaniline by slow release approach. ENVIRONMENTAL SCIENCE AND TECHNOLOGY. 2002;36(21):4698–704.
MLA
Boon, Nico, Leen De Gelder, Hanne Lievens, et al. “Bioaugmenting Bioreactors for the Continuous Removal of 3-chloroaniline by Slow Release Approach.” ENVIRONMENTAL SCIENCE AND TECHNOLOGY 36.21 (2002): 4698–4704. Print.