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Identification and characterization of T-DNA inserts by T-DNA fingerprinting

Ilse Theuns, Pieter Windels, Sylvie De Buck UGent, Anna Depicker UGent, Erik Van Bockstaele and Marc De Loose UGent (2002) EUPHYTICA. 123(1). p.75-84
abstract
A T-DNA fingerprinting method is presented based on amplified fragment length polymorphism with an anchored polymerase chain reaction step. This method allows discrimination between different T-DNA inserts in stably transformed plants. The technique was evaluated by analyzing 51 transgenic Arabidopsis lines that had been characterized in detail by genomic blotting. Comparison of the obtained fingerprints with the available integration information demonstrated that fingerprints were correlated to the predicted patterns, except for the inverted repeat junctions and for those inserts with large deletions at the left or right border. Our experiments show that by using T-DNA fingerprinting multi-copy transgenic lines can be eliminated efficiently so that the technique can be used to enrich a population of transgenic plants for putative single-copy transformants.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
anchored PCR, AFLP, GMO detection, T-DNA integration, AGROBACTERIUM-TUMEFACIENS, ILLEGITIMATE RECOMBINATION, TRANSGENIC PLANTS, BINARY VECTOR, COPY NUMBER, GENOMIC DNA, INTEGRATION, REPEATS, SEQUENCES, TOBACCO
journal title
EUPHYTICA
Euphytica
volume
123
issue
1
pages
75 - 84
Web of Science type
Article
Web of Science id
000174573800009
JCR category
HORTICULTURE
JCR impact factor
0.716 (2002)
JCR rank
7/20 (2002)
JCR quartile
2 (2002)
ISSN
0014-2336
DOI
10.1023/A:1014415619527
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
156900
handle
http://hdl.handle.net/1854/LU-156900
date created
2004-01-14 13:39:00
date last changed
2016-12-19 15:38:23
@article{156900,
  abstract     = {A T-DNA fingerprinting method is presented based on amplified fragment length polymorphism with an anchored polymerase chain reaction step. This method allows discrimination between different T-DNA inserts in stably transformed plants. The technique was evaluated by analyzing 51 transgenic Arabidopsis lines that had been characterized in detail by genomic blotting. Comparison of the obtained fingerprints with the available integration information demonstrated that fingerprints were correlated to the predicted patterns, except for the inverted repeat junctions and for those inserts with large deletions at the left or right border. Our experiments show that by using T-DNA fingerprinting multi-copy transgenic lines can be eliminated efficiently so that the technique can be used to enrich a population of transgenic plants for putative single-copy transformants.},
  author       = {Theuns, Ilse and Windels, Pieter and De Buck, Sylvie and Depicker, Anna and Van Bockstaele, Erik and De Loose, Marc},
  issn         = {0014-2336},
  journal      = {EUPHYTICA},
  keyword      = {anchored PCR,AFLP,GMO detection,T-DNA integration,AGROBACTERIUM-TUMEFACIENS,ILLEGITIMATE RECOMBINATION,TRANSGENIC PLANTS,BINARY VECTOR,COPY NUMBER,GENOMIC DNA,INTEGRATION,REPEATS,SEQUENCES,TOBACCO},
  language     = {eng},
  number       = {1},
  pages        = {75--84},
  title        = {Identification and characterization of T-DNA inserts by T-DNA fingerprinting},
  url          = {http://dx.doi.org/10.1023/A:1014415619527},
  volume       = {123},
  year         = {2002},
}

Chicago
Theuns, Ilse, Pieter Windels, Sylvie De Buck, Anna Depicker, Erik Van Bockstaele, and Marc De Loose. 2002. “Identification and Characterization of T-DNA Inserts by T-DNA Fingerprinting.” Euphytica 123 (1): 75–84.
APA
Theuns, I., Windels, P., De Buck, S., Depicker, A., Van Bockstaele, E., & De Loose, M. (2002). Identification and characterization of T-DNA inserts by T-DNA fingerprinting. EUPHYTICA, 123(1), 75–84.
Vancouver
1.
Theuns I, Windels P, De Buck S, Depicker A, Van Bockstaele E, De Loose M. Identification and characterization of T-DNA inserts by T-DNA fingerprinting. EUPHYTICA. 2002;123(1):75–84.
MLA
Theuns, Ilse, Pieter Windels, Sylvie De Buck, et al. “Identification and Characterization of T-DNA Inserts by T-DNA Fingerprinting.” EUPHYTICA 123.1 (2002): 75–84. Print.