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Identification and characterization of T-DNA inserts by T-DNA fingerprinting

(2002) EUPHYTICA. 123(1). p.75-84
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Abstract
A T-DNA fingerprinting method is presented based on amplified fragment length polymorphism with an anchored polymerase chain reaction step. This method allows discrimination between different T-DNA inserts in stably transformed plants. The technique was evaluated by analyzing 51 transgenic Arabidopsis lines that had been characterized in detail by genomic blotting. Comparison of the obtained fingerprints with the available integration information demonstrated that fingerprints were correlated to the predicted patterns, except for the inverted repeat junctions and for those inserts with large deletions at the left or right border. Our experiments show that by using T-DNA fingerprinting multi-copy transgenic lines can be eliminated efficiently so that the technique can be used to enrich a population of transgenic plants for putative single-copy transformants.
Keywords
anchored PCR, AFLP, GMO detection, T-DNA integration, AGROBACTERIUM-TUMEFACIENS, ILLEGITIMATE RECOMBINATION, TRANSGENIC PLANTS, BINARY VECTOR, COPY NUMBER, GENOMIC DNA, INTEGRATION, REPEATS, SEQUENCES, TOBACCO

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Chicago
Theuns, Ilse, Pieter Windels, Sylvie De Buck, Anna Depicker, Erik Van Bockstaele, and Marc De Loose. 2002. “Identification and Characterization of T-DNA Inserts by T-DNA Fingerprinting.” Euphytica 123 (1): 75–84.
APA
Theuns, I., Windels, P., De Buck, S., Depicker, A., Van Bockstaele, E., & De Loose, M. (2002). Identification and characterization of T-DNA inserts by T-DNA fingerprinting. EUPHYTICA, 123(1), 75–84.
Vancouver
1.
Theuns I, Windels P, De Buck S, Depicker A, Van Bockstaele E, De Loose M. Identification and characterization of T-DNA inserts by T-DNA fingerprinting. EUPHYTICA. 2002;123(1):75–84.
MLA
Theuns, Ilse, Pieter Windels, Sylvie De Buck, et al. “Identification and Characterization of T-DNA Inserts by T-DNA Fingerprinting.” EUPHYTICA 123.1 (2002): 75–84. Print.
@article{156900,
  abstract     = {A T-DNA fingerprinting method is presented based on amplified fragment length polymorphism with an anchored polymerase chain reaction step. This method allows discrimination between different T-DNA inserts in stably transformed plants. The technique was evaluated by analyzing 51 transgenic Arabidopsis lines that had been characterized in detail by genomic blotting. Comparison of the obtained fingerprints with the available integration information demonstrated that fingerprints were correlated to the predicted patterns, except for the inverted repeat junctions and for those inserts with large deletions at the left or right border. Our experiments show that by using T-DNA fingerprinting multi-copy transgenic lines can be eliminated efficiently so that the technique can be used to enrich a population of transgenic plants for putative single-copy transformants.},
  author       = {Theuns, Ilse and Windels, Pieter and De Buck, Sylvie and Depicker, Anna and Van Bockstaele, Erik and De Loose, Marc},
  issn         = {0014-2336},
  journal      = {EUPHYTICA},
  keyword      = {anchored PCR,AFLP,GMO detection,T-DNA integration,AGROBACTERIUM-TUMEFACIENS,ILLEGITIMATE RECOMBINATION,TRANSGENIC PLANTS,BINARY VECTOR,COPY NUMBER,GENOMIC DNA,INTEGRATION,REPEATS,SEQUENCES,TOBACCO},
  language     = {eng},
  number       = {1},
  pages        = {75--84},
  title        = {Identification and characterization of T-DNA inserts by T-DNA fingerprinting},
  url          = {http://dx.doi.org/10.1023/A:1014415619527},
  volume       = {123},
  year         = {2002},
}

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