Ghent University Academic Bibliography

Advanced

A matrix-assisted laser desorption ionization post-source decay (MALDI-PSD) analysis of proteins released from isolated liver mitochondria treated with recombinant truncated Bid

Geert van Loo UGent, Hans Demol UGent, Maria Van Gurp, B Hoorelbeke, Peter Schotte, Rudi Beyaert UGent, B Zhivotovsky, Kris Gevaert UGent, Wim Declercq UGent, Joël Vandekerckhove, et al. (2002) CELL DEATH AND DIFFERENTIATION. 9(3). p.301-308
abstract
A crucial event in the process of apoptosis is caspase-dependent generation of truncated Bid (tBid), inducing release of cytochrome c. In an in vitro reconstitution system we combined purified recombinant tBid with isolated liver mitochondria and identified the released proteins using a proteomic matrix-assisted laser desorption ionization post-source decay (MALDI-PSD) approach. In order to meet physiological conditions, the concentration of tBid was chosen such that it was unable to induce cytochrome c release in mitochondria derived from liver-specific Bcl-2-transgenic mice. Several mitochondrial proteins were identified to be released in a tBid-dependent way, among which cytochrome c, DIABLO/Smac, adenylate kinase 2, acyl-CoA-binding protein, endonuclease G, polypyrimidine tract-binding protein, a type-I RNA helicase, a WD-40 repeat-containing protein and the serine protease Omi. Western blotting confirmed the absence of adenylate kinase 3, a matrix mitochondrial protein. These results demonstrate that a physiologically relevant concentration of tBld is sufficient to induce release of particular intermembrane mitochondrial proteins belonging to a broad molecular-mass range.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ENDOPLASMIC-RETICULUM, CASPASE ACTIVATION, PERMEABILITY TRANSITION, CELL-DEATH, WD-40 REPEAT REGION, BCL-2 FAMILY MEMBERS, CYTOCHROME-C RELEASE, tBid, mitochondria, MALDI-PSD, apoptosis, caspase, ADENYLATE KINASE, STRUCTURAL BASIS, SERINE-PROTEASE
journal title
CELL DEATH AND DIFFERENTIATION
Cell Death Differ.
volume
9
issue
3
pages
301 - 308
Web of Science type
Article
Web of Science id
000174032600010
JCR category
BIOCHEMISTRY & MOLECULAR BIOLOGY
JCR impact factor
5.701 (2002)
JCR rank
35/263 (2002)
JCR quartile
1 (2002)
ISSN
1350-9047
DOI
10.1038/sj.cdd.4400966
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
156373
handle
http://hdl.handle.net/1854/LU-156373
date created
2004-01-14 13:39:00
date last changed
2016-12-19 15:38:18
@article{156373,
  abstract     = {A crucial event in the process of apoptosis is caspase-dependent generation of truncated Bid (tBid), inducing release of cytochrome c. In an in vitro reconstitution system we combined purified recombinant tBid with isolated liver mitochondria and identified the released proteins using a proteomic matrix-assisted laser desorption ionization post-source decay (MALDI-PSD) approach. In order to meet physiological conditions, the concentration of tBid was chosen such that it was unable to induce cytochrome c release in mitochondria derived from liver-specific Bcl-2-transgenic mice. Several mitochondrial proteins were identified to be released in a tBid-dependent way, among which cytochrome c, DIABLO/Smac, adenylate kinase 2, acyl-CoA-binding protein, endonuclease G, polypyrimidine tract-binding protein, a type-I RNA helicase, a WD-40 repeat-containing protein and the serine protease Omi. Western blotting confirmed the absence of adenylate kinase 3, a matrix mitochondrial protein. These results demonstrate that a physiologically relevant concentration of tBld is sufficient to induce release of particular intermembrane mitochondrial proteins belonging to a broad molecular-mass range.},
  author       = {van Loo, Geert and Demol, Hans and Van Gurp, Maria and Hoorelbeke, B and Schotte, Peter and Beyaert, Rudi and Zhivotovsky, B and Gevaert, Kris and Declercq, Wim and Vandekerckhove, Jo{\"e}l and Vandenabeele, Peter},
  issn         = {1350-9047},
  journal      = {CELL DEATH AND DIFFERENTIATION},
  keyword      = {ENDOPLASMIC-RETICULUM,CASPASE ACTIVATION,PERMEABILITY TRANSITION,CELL-DEATH,WD-40 REPEAT REGION,BCL-2 FAMILY MEMBERS,CYTOCHROME-C RELEASE,tBid,mitochondria,MALDI-PSD,apoptosis,caspase,ADENYLATE KINASE,STRUCTURAL BASIS,SERINE-PROTEASE},
  language     = {eng},
  number       = {3},
  pages        = {301--308},
  title        = {A matrix-assisted laser desorption ionization post-source decay (MALDI-PSD) analysis of proteins released from isolated liver mitochondria treated with recombinant truncated Bid},
  url          = {http://dx.doi.org/10.1038/sj.cdd.4400966},
  volume       = {9},
  year         = {2002},
}

Chicago
van Loo, Geert, Hans Demol, Maria Van Gurp, B Hoorelbeke, Peter Schotte, Rudi Beyaert, B Zhivotovsky, et al. 2002. “A Matrix-assisted Laser Desorption Ionization Post-source Decay (MALDI-PSD) Analysis of Proteins Released from Isolated Liver Mitochondria Treated with Recombinant Truncated Bid.” Cell Death and Differentiation 9 (3): 301–308.
APA
van Loo, Geert, Demol, H., Van Gurp, M., Hoorelbeke, B., Schotte, P., Beyaert, R., Zhivotovsky, B., et al. (2002). A matrix-assisted laser desorption ionization post-source decay (MALDI-PSD) analysis of proteins released from isolated liver mitochondria treated with recombinant truncated Bid. CELL DEATH AND DIFFERENTIATION, 9(3), 301–308.
Vancouver
1.
van Loo G, Demol H, Van Gurp M, Hoorelbeke B, Schotte P, Beyaert R, et al. A matrix-assisted laser desorption ionization post-source decay (MALDI-PSD) analysis of proteins released from isolated liver mitochondria treated with recombinant truncated Bid. CELL DEATH AND DIFFERENTIATION. 2002;9(3):301–8.
MLA
van Loo, Geert, Hans Demol, Maria Van Gurp, et al. “A Matrix-assisted Laser Desorption Ionization Post-source Decay (MALDI-PSD) Analysis of Proteins Released from Isolated Liver Mitochondria Treated with Recombinant Truncated Bid.” CELL DEATH AND DIFFERENTIATION 9.3 (2002): 301–308. Print.