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Comprehensive analysis of gene expression in Nicotiana tabacum leaves acclimated to oxidative stress

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Abstract
The molecular mechanisms by which plants acclimate to oxidative stress are poorly understood. To identify the processes involved in acclimation, we performed a comprehensive analysis of gene expression In Nicotiana tabacum leaves acclimated to oxidative stress. Combining mRNA differential display and cDNA array analysis, we estimated that at least 95 genes alter their expression in tobacco leaves acclimated to oxidative stress, of which 83% are induced and 17% repressed. Sequence analysis of 53 sequence tags revealed that in addition to antioxidant genes, genes implicated in abiotic and biotic stress defenses, cellular protection and detoxification, energy and carbohydrate metabolism, de novo protein synthesis, and signal transduction showed altered expression. Expression of most of the genes was enhanced, except for genes associated with photosynthesis and light-regulated processes that were repressed. During acclimation, two distinct groups of coregulated genes ("early-" and "late-response" gene regulons) were observed, indicating the presence of at least two different gene induction pathways. These two gene regulons also showed differential expression patterns on an oxidative stress challenge. Expression of "late-response" genes was augmented in the acclimated leaf tissues, whereas expression of "early-response" genes was not. Together, our data suggest that acclimation to oxidative stress is a highly complex process associated with broad gene expression adjustments. Moreover, our data indicate that in addition to defense gene induction, sensitization of plants for potentiated gene expression might be an important factor in oxidative stress acclimation.
Keywords
PLANTS, TOBACCO, ARABIDOPSIS, DEFENSE-MECHANISMS, SYSTEMIC RESISTANCE, SIGNALING PATHWAYS, DISEASE RESISTANCE, ACQUIRED-RESISTANCE, EXCESS EXCITATION-ENERGY, HEAT-SHOCK PROTEINS

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MLA
Vranová, Eva, Sopagan Atichartpongkul, Raimundo Villarroel-Mandiola, et al. “Comprehensive Analysis of Gene Expression in Nicotiana Tabacum Leaves Acclimated to Oxidative Stress.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 99.16 (2002): 10870–10875. Print.
APA
Vranová, E., Atichartpongkul, S., Villarroel-Mandiola, R., Van Montagu, M., Inzé, D., & Van Camp, W. (2002). Comprehensive analysis of gene expression in Nicotiana tabacum leaves acclimated to oxidative stress. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 99(16), 10870–10875.
Chicago author-date
Vranová, Eva, Sopagan Atichartpongkul, Raimundo Villarroel-Mandiola, Marc Van Montagu, Dirk Inzé, and Wim Van Camp. 2002. “Comprehensive Analysis of Gene Expression in Nicotiana Tabacum Leaves Acclimated to Oxidative Stress.” Proceedings of the National Academy of Sciences of the United States of America 99 (16): 10870–10875.
Chicago author-date (all authors)
Vranová, Eva, Sopagan Atichartpongkul, Raimundo Villarroel-Mandiola, Marc Van Montagu, Dirk Inzé, and Wim Van Camp. 2002. “Comprehensive Analysis of Gene Expression in Nicotiana Tabacum Leaves Acclimated to Oxidative Stress.” Proceedings of the National Academy of Sciences of the United States of America 99 (16): 10870–10875.
Vancouver
1.
Vranová E, Atichartpongkul S, Villarroel-Mandiola R, Van Montagu M, Inzé D, Van Camp W. Comprehensive analysis of gene expression in Nicotiana tabacum leaves acclimated to oxidative stress. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2002;99(16):10870–5.
IEEE
[1]
E. Vranová, S. Atichartpongkul, R. Villarroel-Mandiola, M. Van Montagu, D. Inzé, and W. Van Camp, “Comprehensive analysis of gene expression in Nicotiana tabacum leaves acclimated to oxidative stress,” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 99, no. 16, pp. 10870–10875, 2002.
@article{154152,
  abstract     = {The molecular mechanisms by which plants acclimate to oxidative stress are poorly understood. To identify the processes involved in acclimation, we performed a comprehensive analysis of gene expression In Nicotiana tabacum leaves acclimated to oxidative stress. Combining mRNA differential display and cDNA array analysis, we estimated that at least 95 genes alter their expression in tobacco leaves acclimated to oxidative stress, of which 83% are induced and 17% repressed. Sequence analysis of 53 sequence tags revealed that in addition to antioxidant genes, genes implicated in abiotic and biotic stress defenses, cellular protection and detoxification, energy and carbohydrate metabolism, de novo protein synthesis, and signal transduction showed altered expression. Expression of most of the genes was enhanced, except for genes associated with photosynthesis and light-regulated processes that were repressed. During acclimation, two distinct groups of coregulated genes ("early-" and "late-response" gene regulons) were observed, indicating the presence of at least two different gene induction pathways. These two gene regulons also showed differential expression patterns on an oxidative stress challenge. Expression of "late-response" genes was augmented in the acclimated leaf tissues, whereas expression of "early-response" genes was not. Together, our data suggest that acclimation to oxidative stress is a highly complex process associated with broad gene expression adjustments. Moreover, our data indicate that in addition to defense gene induction, sensitization of plants for potentiated gene expression might be an important factor in oxidative stress acclimation.},
  author       = {Vranová, Eva and Atichartpongkul, Sopagan and Villarroel-Mandiola, Raimundo and Van Montagu, Marc and Inzé, Dirk and Van Camp, Wim},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keywords     = {PLANTS,TOBACCO,ARABIDOPSIS,DEFENSE-MECHANISMS,SYSTEMIC RESISTANCE,SIGNALING PATHWAYS,DISEASE RESISTANCE,ACQUIRED-RESISTANCE,EXCESS EXCITATION-ENERGY,HEAT-SHOCK PROTEINS},
  language     = {eng},
  number       = {16},
  pages        = {10870--10875},
  title        = {Comprehensive analysis of gene expression in Nicotiana tabacum leaves acclimated to oxidative stress},
  url          = {http://dx.doi.org/10.1073/pnas.152337999},
  volume       = {99},
  year         = {2002},
}

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