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Differential effect of jasmonic acid and abscisic acid on cell cycle progression in tobacco BY-2 cells

(2002) PLANT PHYSIOLOGY. 128(1). p.201-211
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Abstract
Environmental stress affects plant growth and development. Several plant hormones, such as salicylic acid, abscisic acid (ABA), jasmonic acid (JA), and ethylene play a crucial role in altering plant morphology in response to stress. Developmental regulation often has the cell cycle machinery among its targets. We analyzed the effect of JA and ABA on cell cycle progression in synchronized tobacco (Nicotiana tabacum) BY-2 cells. Both compounds were found to prevent DNA replication, keeping the cells in the G1 stage, when applied just before the G1/S transition. However, ABA did not have any effect on subsequent phases of the cell cycle when applied at a later stage, whereas JA effectively prevented mitosis on application during DNA synthesis. This demonstrates that JA treatment can freeze synchronized BY-2 cells in both the G1 and G2 stages of the cell cycle. Jasmonate administered after the S-phase was less effective in decreasing the mitotic index, suggesting that cell sensitivity toward JA is dependent on the cell cycle phase. In cultures detained in the G2-phase, we observed a reduced histone HI kinase activity of kinases associated with the p13(sucl) protein.
Keywords
OXIDATIVE STRESS, POSSIBLE INVOLVEMENT, ARABIDOPSIS-THALIANA, METHYL-JASMONATE, SIGNAL-TRANSDUCTION PATHWAYS, PROTEIN-KINASE, GENE-EXPRESSION, HIGHER-PLANTS, WATER-STRESS, CYTOKININ

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Chicago
Świątek, Agnieszka, Marc Lenjou, Dirk Van Bockstaele, Dirk Inzé, and Harry Van Onckelen. 2002. “Differential Effect of Jasmonic Acid and Abscisic Acid on Cell Cycle Progression in Tobacco BY-2 Cells.” Plant Physiology 128 (1): 201–211.
APA
Świątek, A., Lenjou, M., Van Bockstaele, D., Inzé, D., & Van Onckelen, H. (2002). Differential effect of jasmonic acid and abscisic acid on cell cycle progression in tobacco BY-2 cells. PLANT PHYSIOLOGY, 128(1), 201–211.
Vancouver
1.
Świątek A, Lenjou M, Van Bockstaele D, Inzé D, Van Onckelen H. Differential effect of jasmonic acid and abscisic acid on cell cycle progression in tobacco BY-2 cells. PLANT PHYSIOLOGY. 2002;128(1):201–11.
MLA
Świątek, Agnieszka, Marc Lenjou, Dirk Van Bockstaele, et al. “Differential Effect of Jasmonic Acid and Abscisic Acid on Cell Cycle Progression in Tobacco BY-2 Cells.” PLANT PHYSIOLOGY 128.1 (2002): 201–211. Print.
@article{154089,
  abstract     = {Environmental stress affects plant growth and development. Several plant hormones, such as salicylic acid, abscisic acid (ABA), jasmonic acid (JA), and ethylene play a crucial role in altering plant morphology in response to stress. Developmental regulation often has the cell cycle machinery among its targets. We analyzed the effect of JA and ABA on cell cycle progression in synchronized tobacco (Nicotiana tabacum) BY-2 cells. Both compounds were found to prevent DNA replication, keeping the cells in the G1 stage, when applied just before the G1/S transition. However, ABA did not have any effect on subsequent phases of the cell cycle when applied at a later stage, whereas JA effectively prevented mitosis on application during DNA synthesis. This demonstrates that JA treatment can freeze synchronized BY-2 cells in both the G1 and G2 stages of the cell cycle. Jasmonate administered after the S-phase was less effective in decreasing the mitotic index, suggesting that cell sensitivity toward JA is dependent on the cell cycle phase. In cultures detained in the G2-phase, we observed a reduced histone HI kinase activity of kinases associated with the p13(sucl) protein.},
  author       = {\'{S}wi\k{a}tek, Agnieszka and Lenjou, Marc and Van Bockstaele, Dirk and Inz{\'e}, Dirk and Van Onckelen, Harry},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {OXIDATIVE STRESS,POSSIBLE INVOLVEMENT,ARABIDOPSIS-THALIANA,METHYL-JASMONATE,SIGNAL-TRANSDUCTION PATHWAYS,PROTEIN-KINASE,GENE-EXPRESSION,HIGHER-PLANTS,WATER-STRESS,CYTOKININ},
  language     = {eng},
  number       = {1},
  pages        = {201--211},
  title        = {Differential effect of jasmonic acid and abscisic acid on cell cycle progression in tobacco BY-2 cells},
  url          = {http://dx.doi.org/10.1104/pp.010592},
  volume       = {128},
  year         = {2002},
}

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