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Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12

Jan Smalle UGent, Jasmina Kurepa UGent, Peizhen Yang, Elena Babiychuk UGent, Sergei Kushnir UGent, Adam Durski and Richard D Vierstra (2002) PLANT CELL. 14(1). p.17-32
abstract
The 26S proteasome is an ATP-dependent eukaryotic protease responsible for degrading many important cell regulators, especially those conjugated with multiple ubiquitins. Bound on both ends of the 20S core protease is a multisubunit regulatory particle that plays a crucial role in substrate selection by an as yet unknown mechanism(s). Here, we show that the RPN12 subunit of the Arabidopsis regulatory particle is involved in cytokinin responses. A T-DNA insertion mutant that affects RPN12a has a decreased rate of leaf formation, reduced root elongation, delayed skotomorphogenesis, and altered growth responses to exogenous cytokinins, suggesting that the mutant has decreased sensitivity to the hormone. The cytokinin-inducible genes CYCD3 and NIA1 are upregulated constitutively in rpn12a-1, indicating that feedback-inhibitory mechanisms also may be altered. rpn12a-1 seedlings also showed changes in auxin-induced growth responses, further illustrating the close interaction between auxin and cytokinin regulation. In yeast, RPN12 is necessary for the G1/S and G2/M transitions of the cell cycle, phases that have been shown to be under cytokinin control in plants. We propose that RPN12a is part of the Arabidopsis 26S proteasome that controls the stability of one or more of the factors involved in cytokinin regulation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GENE, DE-ETIOLATION, THALIANA, UBIQUITIN SYSTEM, PROTEIN-DEGRADATION, DEPENDENT PROTEOLYSIS, CELL-CYCLE, REGULATORY PARTICLE, MULTIUBIQUITIN-CHAIN-BINDING, SACCHAROMYCES-CEREVISIAE
journal title
PLANT CELL
Plant Cell
volume
14
issue
1
pages
17 - 32
Web of Science type
Article
Web of Science id
000173687000003
JCR category
PLANT SCIENCES
JCR impact factor
10.751 (2002)
JCR rank
3/134 (2002)
JCR quartile
1 (2002)
ISSN
1040-4651
DOI
10.1105/tpc.010381
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
154054
handle
http://hdl.handle.net/1854/LU-154054
date created
2004-01-14 13:39:00
date last changed
2016-12-19 15:38:54
@article{154054,
  abstract     = {The 26S proteasome is an ATP-dependent eukaryotic protease responsible for degrading many important cell regulators, especially those conjugated with multiple ubiquitins. Bound on both ends of the 20S core protease is a multisubunit regulatory particle that plays a crucial role in substrate selection by an as yet unknown mechanism(s). Here, we show that the RPN12 subunit of the Arabidopsis regulatory particle is involved in cytokinin responses. A T-DNA insertion mutant that affects RPN12a has a decreased rate of leaf formation, reduced root elongation, delayed skotomorphogenesis, and altered growth responses to exogenous cytokinins, suggesting that the mutant has decreased sensitivity to the hormone. The cytokinin-inducible genes CYCD3 and NIA1 are upregulated constitutively in rpn12a-1, indicating that feedback-inhibitory mechanisms also may be altered. rpn12a-1 seedlings also showed changes in auxin-induced growth responses, further illustrating the close interaction between auxin and cytokinin regulation. In yeast, RPN12 is necessary for the G1/S and G2/M transitions of the cell cycle, phases that have been shown to be under cytokinin control in plants. We propose that RPN12a is part of the Arabidopsis 26S proteasome that controls the stability of one or more of the factors involved in cytokinin regulation.},
  author       = {Smalle, Jan and Kurepa, Jasmina and Yang, Peizhen and Babiychuk, Elena and Kushnir, Sergei and Durski, Adam and Vierstra, Richard D},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {GENE,DE-ETIOLATION,THALIANA,UBIQUITIN SYSTEM,PROTEIN-DEGRADATION,DEPENDENT PROTEOLYSIS,CELL-CYCLE,REGULATORY PARTICLE,MULTIUBIQUITIN-CHAIN-BINDING,SACCHAROMYCES-CEREVISIAE},
  language     = {eng},
  number       = {1},
  pages        = {17--32},
  title        = {Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12},
  url          = {http://dx.doi.org/10.1105/tpc.010381},
  volume       = {14},
  year         = {2002},
}

Chicago
Smalle, Jan, Jasmina Kurepa, Peizhen Yang, Elena Babiychuk, Sergei Kushnir, Adam Durski, and Richard D Vierstra. 2002. “Cytokinin Growth Responses in Arabidopsis Involve the 26S Proteasome Subunit RPN12.” Plant Cell 14 (1): 17–32.
APA
Smalle, J., Kurepa, J., Yang, P., Babiychuk, E., Kushnir, S., Durski, A., & Vierstra, R. D. (2002). Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. PLANT CELL, 14(1), 17–32.
Vancouver
1.
Smalle J, Kurepa J, Yang P, Babiychuk E, Kushnir S, Durski A, et al. Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. PLANT CELL. 2002;14(1):17–32.
MLA
Smalle, Jan, Jasmina Kurepa, Peizhen Yang, et al. “Cytokinin Growth Responses in Arabidopsis Involve the 26S Proteasome Subunit RPN12.” PLANT CELL 14.1 (2002): 17–32. Print.