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Effect of auxin, cytokinin, and sucrose on cell cycle gene expression in Arabidopsis thaliana cell suspension cultures

Caroline Richard (UGent) , Magali Lescot (UGent) , Dirk Inzé (UGent) and Lieven De Veylder (UGent)
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Abstract
Cell suspension cultures of Arabidopsis thaliana (L.) Heynh. have been used to study the effect of auxin (alpha-naphthaleneacetic acid), cytokinin (kinetin), and sucrose on cell cycle gene expression. Auxin, cytokinin, and/or sucrose were re-added to culture media depleted for all three mitogens and the effects on the transcript levels of key cell cycle genes were monitored. Different cell cycle genes responded differentially to the mitogen supply. The cyclin-dependent kinase (CDK)-encoding gene CDKA;1 and the A- and D-type cyclins were induced by sucrose, whereas CDKB1;1 and CYCB1;1 were induced most strongly by the combined administration of auxin and cytokinin. The transcript levels of CDK inhibitors were up-regulated upon the addition of Gamborg's B5 salts to depleted medium. This induction was counteracted by the presence of auxin, cytokinin, or sucrose, depending on the CDK inhibitor. The obtained results are discussed in regard to the current knowledge on mitogen effects on cell cycle regulation in Arabidopsis.
Keywords
PROMOTER, PLANTS, INHIBITOR, GROWTH, DIVISION, G1 PHASE, ABSCISIC-ACID, PROTEIN-KINASE, DEVELOPMENTAL EXPRESSION, DEPENDENT KINASES, transcriptional regulation, hormones, cyclin, CDK, cell division

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Chicago
Richard, Caroline, Magali Lescot, Dirk Inzé, and Lieven De Veylder. 2002. “Effect of Auxin, Cytokinin, and Sucrose on Cell Cycle Gene Expression in Arabidopsis Thaliana Cell Suspension Cultures.” Plant Cell Tissue and Organ Culture 69 (2): 167–176.
APA
Richard, C., Lescot, M., Inzé, D., & De Veylder, L. (2002). Effect of auxin, cytokinin, and sucrose on cell cycle gene expression in Arabidopsis thaliana cell suspension cultures. PLANT CELL TISSUE AND ORGAN CULTURE, 69(2), 167–176.
Vancouver
1.
Richard C, Lescot M, Inzé D, De Veylder L. Effect of auxin, cytokinin, and sucrose on cell cycle gene expression in Arabidopsis thaliana cell suspension cultures. PLANT CELL TISSUE AND ORGAN CULTURE. 2002;69(2):167–76.
MLA
Richard, Caroline, Magali Lescot, Dirk Inzé, et al. “Effect of Auxin, Cytokinin, and Sucrose on Cell Cycle Gene Expression in Arabidopsis Thaliana Cell Suspension Cultures.” PLANT CELL TISSUE AND ORGAN CULTURE 69.2 (2002): 167–176. Print.
@article{154023,
  abstract     = {Cell suspension cultures of Arabidopsis thaliana (L.) Heynh. have been used to study the effect of auxin (alpha-naphthaleneacetic acid), cytokinin (kinetin), and sucrose on cell cycle gene expression. Auxin, cytokinin, and/or sucrose were re-added to culture media depleted for all three mitogens and the effects on the transcript levels of key cell cycle genes were monitored. Different cell cycle genes responded differentially to the mitogen supply. The cyclin-dependent kinase (CDK)-encoding gene CDKA;1 and the A- and D-type cyclins were induced by sucrose, whereas CDKB1;1 and CYCB1;1 were induced most strongly by the combined administration of auxin and cytokinin. The transcript levels of CDK inhibitors were up-regulated upon the addition of Gamborg's B5 salts to depleted medium. This induction was counteracted by the presence of auxin, cytokinin, or sucrose, depending on the CDK inhibitor. The obtained results are discussed in regard to the current knowledge on mitogen effects on cell cycle regulation in Arabidopsis.},
  author       = {Richard, Caroline and Lescot, Magali and Inz{\'e}, Dirk and De Veylder, Lieven},
  issn         = {0167-6857},
  journal      = {PLANT CELL TISSUE AND ORGAN CULTURE},
  keyword      = {PROMOTER,PLANTS,INHIBITOR,GROWTH,DIVISION,G1 PHASE,ABSCISIC-ACID,PROTEIN-KINASE,DEVELOPMENTAL EXPRESSION,DEPENDENT KINASES,transcriptional regulation,hormones,cyclin,CDK,cell division},
  language     = {eng},
  number       = {2},
  pages        = {167--176},
  title        = {Effect of auxin, cytokinin, and sucrose on cell cycle gene expression in Arabidopsis thaliana cell suspension cultures},
  url          = {http://dx.doi.org/10.1023/A:1015241709145},
  volume       = {69},
  year         = {2002},
}

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