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Effect of auxin, cytokinin, and sucrose on cell cycle gene expression in Arabidopsis thaliana cell suspension cultures

Caroline Richard UGent, Magali Lescot UGent, Dirk Inzé UGent and Lieven De Veylder UGent (2002) PLANT CELL TISSUE AND ORGAN CULTURE. 69(2). p.167-176
abstract
Cell suspension cultures of Arabidopsis thaliana (L.) Heynh. have been used to study the effect of auxin (alpha-naphthaleneacetic acid), cytokinin (kinetin), and sucrose on cell cycle gene expression. Auxin, cytokinin, and/or sucrose were re-added to culture media depleted for all three mitogens and the effects on the transcript levels of key cell cycle genes were monitored. Different cell cycle genes responded differentially to the mitogen supply. The cyclin-dependent kinase (CDK)-encoding gene CDKA;1 and the A- and D-type cyclins were induced by sucrose, whereas CDKB1;1 and CYCB1;1 were induced most strongly by the combined administration of auxin and cytokinin. The transcript levels of CDK inhibitors were up-regulated upon the addition of Gamborg's B5 salts to depleted medium. This induction was counteracted by the presence of auxin, cytokinin, or sucrose, depending on the CDK inhibitor. The obtained results are discussed in regard to the current knowledge on mitogen effects on cell cycle regulation in Arabidopsis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PROMOTER, PLANTS, INHIBITOR, GROWTH, DIVISION, G1 PHASE, ABSCISIC-ACID, PROTEIN-KINASE, DEVELOPMENTAL EXPRESSION, DEPENDENT KINASES, transcriptional regulation, hormones, cyclin, CDK, cell division
journal title
PLANT CELL TISSUE AND ORGAN CULTURE
Plant Cell Tissue Organ Cult.
volume
69
issue
2
pages
167 - 176
Web of Science type
Article
Web of Science id
000175103000008
JCR category
PLANT SCIENCES
JCR impact factor
0.632 (2002)
JCR rank
82/134 (2002)
JCR quartile
3 (2002)
ISSN
0167-6857
DOI
10.1023/A:1015241709145
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
154023
handle
http://hdl.handle.net/1854/LU-154023
date created
2004-01-14 13:39:00
date last changed
2016-12-19 15:38:54
@article{154023,
  abstract     = {Cell suspension cultures of Arabidopsis thaliana (L.) Heynh. have been used to study the effect of auxin (alpha-naphthaleneacetic acid), cytokinin (kinetin), and sucrose on cell cycle gene expression. Auxin, cytokinin, and/or sucrose were re-added to culture media depleted for all three mitogens and the effects on the transcript levels of key cell cycle genes were monitored. Different cell cycle genes responded differentially to the mitogen supply. The cyclin-dependent kinase (CDK)-encoding gene CDKA;1 and the A- and D-type cyclins were induced by sucrose, whereas CDKB1;1 and CYCB1;1 were induced most strongly by the combined administration of auxin and cytokinin. The transcript levels of CDK inhibitors were up-regulated upon the addition of Gamborg's B5 salts to depleted medium. This induction was counteracted by the presence of auxin, cytokinin, or sucrose, depending on the CDK inhibitor. The obtained results are discussed in regard to the current knowledge on mitogen effects on cell cycle regulation in Arabidopsis.},
  author       = {Richard, Caroline and Lescot, Magali and Inz{\'e}, Dirk and De Veylder, Lieven},
  issn         = {0167-6857},
  journal      = {PLANT CELL TISSUE AND ORGAN CULTURE},
  keyword      = {PROMOTER,PLANTS,INHIBITOR,GROWTH,DIVISION,G1 PHASE,ABSCISIC-ACID,PROTEIN-KINASE,DEVELOPMENTAL EXPRESSION,DEPENDENT KINASES,transcriptional regulation,hormones,cyclin,CDK,cell division},
  language     = {eng},
  number       = {2},
  pages        = {167--176},
  title        = {Effect of auxin, cytokinin, and sucrose on cell cycle gene expression in Arabidopsis thaliana cell suspension cultures},
  url          = {http://dx.doi.org/10.1023/A:1015241709145},
  volume       = {69},
  year         = {2002},
}

Chicago
Richard, Caroline, Magali Lescot, Dirk Inzé, and Lieven De Veylder. 2002. “Effect of Auxin, Cytokinin, and Sucrose on Cell Cycle Gene Expression in Arabidopsis Thaliana Cell Suspension Cultures.” Plant Cell Tissue and Organ Culture 69 (2): 167–176.
APA
Richard, C., Lescot, M., Inzé, D., & De Veylder, L. (2002). Effect of auxin, cytokinin, and sucrose on cell cycle gene expression in Arabidopsis thaliana cell suspension cultures. PLANT CELL TISSUE AND ORGAN CULTURE, 69(2), 167–176.
Vancouver
1.
Richard C, Lescot M, Inzé D, De Veylder L. Effect of auxin, cytokinin, and sucrose on cell cycle gene expression in Arabidopsis thaliana cell suspension cultures. PLANT CELL TISSUE AND ORGAN CULTURE. 2002;69(2):167–76.
MLA
Richard, Caroline, Magali Lescot, Dirk Inzé, et al. “Effect of Auxin, Cytokinin, and Sucrose on Cell Cycle Gene Expression in Arabidopsis Thaliana Cell Suspension Cultures.” PLANT CELL TISSUE AND ORGAN CULTURE 69.2 (2002): 167–176. Print.