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The NADPH:quinone oxidoreductase P1-ζ-crystallin in Arabidopsis catalyzes the α,β-hydrogenation of 2-alkenals: detoxication of the lipid peroxide-derived reactive aldehydes

Jun’ichi Mano, Yoshimitsu Torii, Shun-ichiro Hayashi, Koichi Takimoto, Kenji Matsui, Kaoru Nakamura, Dirk Inzé UGent, Elena Babiychuk UGent, Sergei Kushnir UGent and Kozi Asada (2002) PLANT AND CELL PHYSIOLOGY. 43(12). p.1445-1455
abstract
P1-zeta-crystallin (P1-ZCr) is an oxidative stress-induced NADPH:quinone oxidoreductase in Arabidopsis thaliana, but its physiological electron acceptors have not been identified. We found that recombinant P1-ZCr catalyzed the reduction of 2-alkenals of carbon chain C-3-C-9 with NADPH. Among these 2-alkenals, the highest specificity was observed for 4-hydroxy-(2E)-nonenal (HNE), one of the major toxic products generated from lipid peroxides. (3Z)-Hexenal and aldehydes without alpha,beta-unsaturated bonds did not serve as electron acceptors. In the 2-alkenal molecules, P1-ZCr catalyzed the hydrogenation of alpha,beta-unsaturated bonds, but not the reduction of the aldehyde moiety, to produce saturated aldehydes, as determined by gas chromatography/mass spectrometry. We propose the enzyme name NADPH:2-alkenal alpha,beta-hydrogenase (ALH). A major portion of the NADPH-dependent HNE-reducing activity in A. thaliana leaves was inhibited by the specific antiserum against P1-ZCr, indicating that the endogenous P1-ZCr protein has ALH activity. Because expression of the P1-ZCr gene in A. thaliana is induced by oxidative stress treatments, we conclude that P1-ZCr functions as a defense against oxidative stress by scavenging the highly toxic, lipid peroxide-derived alpha,beta-unsaturated aldehydes.
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alternative title
The NADPH:quinone oxidoreductase P1-zeta-crystallin in Arabidopsis catalyzes the alpha,beta-hydrogenation of 2-alkenals : detoxication of the lipid peroxide-derived reactive aldehydes
year
type
journalArticle (original)
publication status
published
subject
keyword
Arabidopsis thaliana, 2-alkenal, antioxidant, 4-hydroxy-(2E)-hexenal, 4-hydroxy-(2E)-nonenal, oxidative stress, GLUTATHIONE-S-TRANSFERASE, INDUCED OXIDATIVE STRESS, OXIDIZING DRUG DIAMIDE, ALCOHOL-DEHYDROGENASE, GROWTH-INHIBITION, ZETA-CRYSTALLIN, CDNA CLONING, CELLS, OXYGENATION, REDUCTASE
journal title
PLANT AND CELL PHYSIOLOGY
Plant Cell Physiol.
volume
43
issue
12
pages
1445 - 1455
Web of Science type
Article
Web of Science id
000180106300006
JCR category
PLANT SCIENCES
JCR impact factor
3.084 (2002)
JCR rank
11/134 (2002)
JCR quartile
1 (2002)
ISSN
0032-0781
DOI
10.1093/pcp/pcf187
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
153948
handle
http://hdl.handle.net/1854/LU-153948
date created
2004-01-14 13:39:00
date last changed
2013-09-26 15:21:31
@article{153948,
  abstract     = {P1-zeta-crystallin (P1-ZCr) is an oxidative stress-induced NADPH:quinone oxidoreductase in Arabidopsis thaliana, but its physiological electron acceptors have not been identified. We found that recombinant P1-ZCr catalyzed the reduction of 2-alkenals of carbon chain C-3-C-9 with NADPH. Among these 2-alkenals, the highest specificity was observed for 4-hydroxy-(2E)-nonenal (HNE), one of the major toxic products generated from lipid peroxides. (3Z)-Hexenal and aldehydes without alpha,beta-unsaturated bonds did not serve as electron acceptors. In the 2-alkenal molecules, P1-ZCr catalyzed the hydrogenation of alpha,beta-unsaturated bonds, but not the reduction of the aldehyde moiety, to produce saturated aldehydes, as determined by gas chromatography/mass spectrometry. We propose the enzyme name NADPH:2-alkenal alpha,beta-hydrogenase (ALH). A major portion of the NADPH-dependent HNE-reducing activity in A. thaliana leaves was inhibited by the specific antiserum against P1-ZCr, indicating that the endogenous P1-ZCr protein has ALH activity. Because expression of the P1-ZCr gene in A. thaliana is induced by oxidative stress treatments, we conclude that P1-ZCr functions as a defense against oxidative stress by scavenging the highly toxic, lipid peroxide-derived alpha,beta-unsaturated aldehydes.},
  author       = {Mano, Jun{\textquoteright}ichi and Torii, Yoshimitsu and Hayashi, Shun-ichiro and Takimoto, Koichi and Matsui, Kenji and Nakamura, Kaoru and Inz{\'e}, Dirk and Babiychuk, Elena and Kushnir, Sergei and Asada, Kozi},
  issn         = {0032-0781},
  journal      = {PLANT AND CELL PHYSIOLOGY},
  keyword      = {Arabidopsis thaliana,2-alkenal,antioxidant,4-hydroxy-(2E)-hexenal,4-hydroxy-(2E)-nonenal,oxidative stress,GLUTATHIONE-S-TRANSFERASE,INDUCED OXIDATIVE STRESS,OXIDIZING DRUG DIAMIDE,ALCOHOL-DEHYDROGENASE,GROWTH-INHIBITION,ZETA-CRYSTALLIN,CDNA CLONING,CELLS,OXYGENATION,REDUCTASE},
  language     = {eng},
  number       = {12},
  pages        = {1445--1455},
  title        = {The NADPH:quinone oxidoreductase P1-\ensuremath{\zeta}-crystallin in Arabidopsis catalyzes the \ensuremath{\alpha},\ensuremath{\beta}-hydrogenation of 2-alkenals: detoxication of the lipid peroxide-derived reactive aldehydes},
  url          = {http://dx.doi.org/10.1093/pcp/pcf187},
  volume       = {43},
  year         = {2002},
}

Chicago
Mano, Jun’ichi, Yoshimitsu Torii, Shun-ichiro Hayashi, Koichi Takimoto, Kenji Matsui, Kaoru Nakamura, Dirk Inzé, Elena Babiychuk, Sergei Kushnir, and Kozi Asada. 2002. “The NADPH:quinone Oxidoreductase P1-ζ-crystallin in Arabidopsis Catalyzes the Α,β-hydrogenation of 2-alkenals: Detoxication of the Lipid Peroxide-derived Reactive Aldehydes.” Plant and Cell Physiology 43 (12): 1445–1455.
APA
Mano, Jun’ichi, Torii, Y., Hayashi, S., Takimoto, K., Matsui, K., Nakamura, K., Inzé, D., et al. (2002). The NADPH:quinone oxidoreductase P1-ζ-crystallin in Arabidopsis catalyzes the α,β-hydrogenation of 2-alkenals: detoxication of the lipid peroxide-derived reactive aldehydes. PLANT AND CELL PHYSIOLOGY, 43(12), 1445–1455.
Vancouver
1.
Mano J, Torii Y, Hayashi S, Takimoto K, Matsui K, Nakamura K, et al. The NADPH:quinone oxidoreductase P1-ζ-crystallin in Arabidopsis catalyzes the α,β-hydrogenation of 2-alkenals: detoxication of the lipid peroxide-derived reactive aldehydes. PLANT AND CELL PHYSIOLOGY. 2002;43(12):1445–55.
MLA
Mano, Jun’ichi, Yoshimitsu Torii, Shun-ichiro Hayashi, et al. “The NADPH:quinone Oxidoreductase P1-ζ-crystallin in Arabidopsis Catalyzes the Α,β-hydrogenation of 2-alkenals: Detoxication of the Lipid Peroxide-derived Reactive Aldehydes.” PLANT AND CELL PHYSIOLOGY 43.12 (2002): 1445–1455. Print.