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An optimized Agrobacterium-mediated transformation procedure for Phaseolus acutifolius A. Gray

Janniek De Clercq (UGent), Mukund Zambre (UGent), Marc Van Montagu (UGent), Willy Dillen (UGent) and Geert Angenon (UGent)
(2002) PLANT CELL REPORTS. 21(4). p.333-340
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Abstract
To improve Agrobacterium tumefaciens-mediated transformation of Phaseolus acutifolius, we examined the effect of different factors on T-DNA transfer by measuring transient expression levels of an intron-containing beta-glucuronidase gene. Improved transformation frequencies were obtained with an A. tumefaciens strain carrying nopaline-type virulence genes and when calli were infected with Agrobacterium cells in the early-log growth phase. Optimized co-cultivation was performed at 22degreesC under a 16/8-h (day/night) photoperiod in an acidic medium (pH 5.5) in the presence of 200 muM acetosyringone. By combining the best treatments, an efficient and reproducible transformation procedure was established for the R acutifolius genotype N1576. Southern and immunoblot analyses confirmed the stable integration and expression of the transgenes in the primary transgenic plants and their progeny.
Keywords
TUMEFACIENS, REGENERATION, EXPRESSION, SATIVUM L., VULGARIS, PLANT-CELLS, STABLE TRANSFORMATION, GENE-TRANSFER EFFICIENCY, COTYLEDONARY NODE TRANSFORMATION, T-DNA TRANSFER, transformation, tepary bean, Phaseolus, Agrobacterium, callus

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Chicago
De Clercq, Janniek, Mukund Zambre, Marc Van Montagu, Willy Dillen, and Geert Angenon. 2002. “An Optimized Agrobacterium-mediated Transformation Procedure for Phaseolus Acutifolius A. Gray.” Plant Cell Reports 21 (4): 333–340.
APA
De Clercq, Janniek, Zambre, M., Van Montagu, M., Dillen, W., & Angenon, G. (2002). An optimized Agrobacterium-mediated transformation procedure for Phaseolus acutifolius A. Gray. PLANT CELL REPORTS, 21(4), 333–340.
Vancouver
1.
De Clercq J, Zambre M, Van Montagu M, Dillen W, Angenon G. An optimized Agrobacterium-mediated transformation procedure for Phaseolus acutifolius A. Gray. PLANT CELL REPORTS. 2002;21(4):333–40.
MLA
De Clercq, Janniek, Mukund Zambre, Marc Van Montagu, et al. “An Optimized Agrobacterium-mediated Transformation Procedure for Phaseolus Acutifolius A. Gray.” PLANT CELL REPORTS 21.4 (2002): 333–340. Print.
@article{153862,
  abstract     = {To improve Agrobacterium tumefaciens-mediated transformation of Phaseolus acutifolius, we examined the effect of different factors on T-DNA transfer by measuring transient expression levels of an intron-containing beta-glucuronidase gene. Improved transformation frequencies were obtained with an A. tumefaciens strain carrying nopaline-type virulence genes and when calli were infected with Agrobacterium cells in the early-log growth phase. Optimized co-cultivation was performed at 22degreesC under a 16/8-h (day/night) photoperiod in an acidic medium (pH 5.5) in the presence of 200 muM acetosyringone. By combining the best treatments, an efficient and reproducible transformation procedure was established for the R acutifolius genotype N1576. Southern and immunoblot analyses confirmed the stable integration and expression of the transgenes in the primary transgenic plants and their progeny.},
  author       = {De Clercq, Janniek and Zambre, Mukund and Van Montagu, Marc and Dillen, Willy and Angenon, Geert},
  issn         = {0721-7714},
  journal      = {PLANT CELL REPORTS},
  keyword      = {TUMEFACIENS,REGENERATION,EXPRESSION,SATIVUM L.,VULGARIS,PLANT-CELLS,STABLE TRANSFORMATION,GENE-TRANSFER EFFICIENCY,COTYLEDONARY NODE TRANSFORMATION,T-DNA TRANSFER,transformation,tepary bean,Phaseolus,Agrobacterium,callus},
  language     = {eng},
  number       = {4},
  pages        = {333--340},
  title        = {An optimized Agrobacterium-mediated transformation procedure for Phaseolus acutifolius A. Gray},
  url          = {http://dx.doi.org/10.1007/s00299-002-0518-0},
  volume       = {21},
  year         = {2002},
}

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