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An optimized Agrobacterium-mediated transformation procedure for Phaseolus acutifolius A. Gray

Janniek De Clercq UGent, Mukund Zambre UGent, Marc Van Montagu UGent, Willy Dillen UGent and Geert Angenon UGent (2002) PLANT CELL REPORTS. 21(4). p.333-340
abstract
To improve Agrobacterium tumefaciens-mediated transformation of Phaseolus acutifolius, we examined the effect of different factors on T-DNA transfer by measuring transient expression levels of an intron-containing beta-glucuronidase gene. Improved transformation frequencies were obtained with an A. tumefaciens strain carrying nopaline-type virulence genes and when calli were infected with Agrobacterium cells in the early-log growth phase. Optimized co-cultivation was performed at 22degreesC under a 16/8-h (day/night) photoperiod in an acidic medium (pH 5.5) in the presence of 200 muM acetosyringone. By combining the best treatments, an efficient and reproducible transformation procedure was established for the R acutifolius genotype N1576. Southern and immunoblot analyses confirmed the stable integration and expression of the transgenes in the primary transgenic plants and their progeny.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
TUMEFACIENS, REGENERATION, EXPRESSION, SATIVUM L., VULGARIS, PLANT-CELLS, STABLE TRANSFORMATION, GENE-TRANSFER EFFICIENCY, COTYLEDONARY NODE TRANSFORMATION, T-DNA TRANSFER, transformation, tepary bean, Phaseolus, Agrobacterium, callus
journal title
PLANT CELL REPORTS
Plant Cell Reports
volume
21
issue
4
pages
333 - 340
Web of Science type
Article
Web of Science id
000179923200007
JCR category
PLANT SCIENCES
JCR impact factor
1.34 (2002)
JCR rank
46/134 (2002)
JCR quartile
2 (2002)
ISSN
0721-7714
DOI
10.1007/s00299-002-0518-0
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
153862
handle
http://hdl.handle.net/1854/LU-153862
date created
2004-01-14 13:38:00
date last changed
2016-12-19 15:38:18
@article{153862,
  abstract     = {To improve Agrobacterium tumefaciens-mediated transformation of Phaseolus acutifolius, we examined the effect of different factors on T-DNA transfer by measuring transient expression levels of an intron-containing beta-glucuronidase gene. Improved transformation frequencies were obtained with an A. tumefaciens strain carrying nopaline-type virulence genes and when calli were infected with Agrobacterium cells in the early-log growth phase. Optimized co-cultivation was performed at 22degreesC under a 16/8-h (day/night) photoperiod in an acidic medium (pH 5.5) in the presence of 200 muM acetosyringone. By combining the best treatments, an efficient and reproducible transformation procedure was established for the R acutifolius genotype N1576. Southern and immunoblot analyses confirmed the stable integration and expression of the transgenes in the primary transgenic plants and their progeny.},
  author       = {De Clercq, Janniek and Zambre, Mukund and Van Montagu, Marc and Dillen, Willy and Angenon, Geert},
  issn         = {0721-7714},
  journal      = {PLANT CELL REPORTS},
  keyword      = {TUMEFACIENS,REGENERATION,EXPRESSION,SATIVUM L.,VULGARIS,PLANT-CELLS,STABLE TRANSFORMATION,GENE-TRANSFER EFFICIENCY,COTYLEDONARY NODE TRANSFORMATION,T-DNA TRANSFER,transformation,tepary bean,Phaseolus,Agrobacterium,callus},
  language     = {eng},
  number       = {4},
  pages        = {333--340},
  title        = {An optimized Agrobacterium-mediated transformation procedure for Phaseolus acutifolius A. Gray},
  url          = {http://dx.doi.org/10.1007/s00299-002-0518-0},
  volume       = {21},
  year         = {2002},
}

Chicago
De Clercq, Janniek, Mukund Zambre, Marc Van Montagu, Willy Dillen, and Geert Angenon. 2002. “An Optimized Agrobacterium-mediated Transformation Procedure for Phaseolus Acutifolius A. Gray.” Plant Cell Reports 21 (4): 333–340.
APA
De Clercq, Janniek, Zambre, M., Van Montagu, M., Dillen, W., & Angenon, G. (2002). An optimized Agrobacterium-mediated transformation procedure for Phaseolus acutifolius A. Gray. PLANT CELL REPORTS, 21(4), 333–340.
Vancouver
1.
De Clercq J, Zambre M, Van Montagu M, Dillen W, Angenon G. An optimized Agrobacterium-mediated transformation procedure for Phaseolus acutifolius A. Gray. PLANT CELL REPORTS. 2002;21(4):333–40.
MLA
De Clercq, Janniek, Mukund Zambre, Marc Van Montagu, et al. “An Optimized Agrobacterium-mediated Transformation Procedure for Phaseolus Acutifolius A. Gray.” PLANT CELL REPORTS 21.4 (2002): 333–340. Print.