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Molecular characterization of plant ubiquitin-conjugating enzymes belonging to the UbcP4/E2-C/UBCx/UbcH10 gene family

(2002) PLANT PHYSIOLOGY. 130(3). p.1230-1240
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Abstract
The anaphase promoting complex or cyclosome is the ubiquitin-ligase that targets destruction box-containing proteins for proteolysis during the cell cycle. Anaphase promoting complex or cyclosome and its activator (the fizzy and fizzy-related) proteins work together with ubiquitin-conjugating enzymes (UBCs) (E2s). One class of E2s (called E2-C) seems specifically involved in cyclin B1 degradation. Although it has recently been shown that mammalian E2-C is regulated at the protein level during the cell cycle, not much is known concerning the expression of these genes. Arabidopsis encodes two genes belonging to the E2-C gene family (called UBC19 and UBC20). We found that UBC19 is able to complement fission yeast (Schizosaccharomyces pombe) UbcP4-140 mutant, indicating that the plant protein can functionally replace its yeast ortholog for protein degradation during mitosis. In situ hybridization experiments were performed to study the expression of the E2-C genes in various tissues of plants. Their transcripts were always, but not exclusively, found in tissues active for cell division. Thus, the UBC19/20 E2s may have a key function during cell cycle, but may also be involved in ubiquitylation reactions occurring during differentiation and/or in differentiated cells. Finally, we showed that a translational fusion protein between UBC19 and green fluorescent protein localized both in the cytosol and the nucleus in stable transformed tobacco (Nicotiana tabacum cv Bright Yellow 2) cells.
Keywords
CYCLE-DEPENDENT PROTEOLYSIS, ANAPHASE-PROMOTING COMPLEX, CELL-CYCLE, ARABIDOPSIS-THALIANA, FISSION YEAST, SACCHAROMYCES-CEREVISIAE, DESTRUCTION MACHINERY, MEDIATED PROTEOLYSIS, MITOTIC CYCLINS, S-PHASE

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Chicago
Criqui, Marie Claire, Janice de Almeida Engler, Alain Camasses, Arnaud Capron, Yves Parmentier, Dirk Inzé, and Pascal Genschik. 2002. “Molecular Characterization of Plant Ubiquitin-conjugating Enzymes Belonging to the UbcP4/E2-C/UBCx/UbcH10 Gene Family.” Plant Physiology 130 (3): 1230–1240.
APA
Criqui, M. C., de Almeida Engler, J., Camasses, A., Capron, A., Parmentier, Y., Inzé, D., & Genschik, P. (2002). Molecular characterization of plant ubiquitin-conjugating enzymes belonging to the UbcP4/E2-C/UBCx/UbcH10 gene family. PLANT PHYSIOLOGY, 130(3), 1230–1240.
Vancouver
1.
Criqui MC, de Almeida Engler J, Camasses A, Capron A, Parmentier Y, Inzé D, et al. Molecular characterization of plant ubiquitin-conjugating enzymes belonging to the UbcP4/E2-C/UBCx/UbcH10 gene family. PLANT PHYSIOLOGY. 2002;130(3):1230–40.
MLA
Criqui, Marie Claire, Janice de Almeida Engler, Alain Camasses, et al. “Molecular Characterization of Plant Ubiquitin-conjugating Enzymes Belonging to the UbcP4/E2-C/UBCx/UbcH10 Gene Family.” PLANT PHYSIOLOGY 130.3 (2002): 1230–1240. Print.
@article{153831,
  abstract     = {The anaphase promoting complex or cyclosome is the ubiquitin-ligase that targets destruction box-containing proteins for proteolysis during the cell cycle. Anaphase promoting complex or cyclosome and its activator (the fizzy and fizzy-related) proteins work together with ubiquitin-conjugating enzymes (UBCs) (E2s). One class of E2s (called E2-C) seems specifically involved in cyclin B1 degradation. Although it has recently been shown that mammalian E2-C is regulated at the protein level during the cell cycle, not much is known concerning the expression of these genes. Arabidopsis encodes two genes belonging to the E2-C gene family (called UBC19 and UBC20). We found that UBC19 is able to complement fission yeast (Schizosaccharomyces pombe) UbcP4-140 mutant, indicating that the plant protein can functionally replace its yeast ortholog for protein degradation during mitosis. In situ hybridization experiments were performed to study the expression of the E2-C genes in various tissues of plants. Their transcripts were always, but not exclusively, found in tissues active for cell division. Thus, the UBC19/20 E2s may have a key function during cell cycle, but may also be involved in ubiquitylation reactions occurring during differentiation and/or in differentiated cells. Finally, we showed that a translational fusion protein between UBC19 and green fluorescent protein localized both in the cytosol and the nucleus in stable transformed tobacco (Nicotiana tabacum cv Bright Yellow 2) cells.},
  author       = {Criqui, Marie Claire and de Almeida Engler, Janice and Camasses, Alain and Capron, Arnaud and Parmentier, Yves and Inz{\'e}, Dirk and Genschik, Pascal},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {CYCLE-DEPENDENT PROTEOLYSIS,ANAPHASE-PROMOTING COMPLEX,CELL-CYCLE,ARABIDOPSIS-THALIANA,FISSION YEAST,SACCHAROMYCES-CEREVISIAE,DESTRUCTION MACHINERY,MEDIATED PROTEOLYSIS,MITOTIC CYCLINS,S-PHASE},
  language     = {eng},
  number       = {3},
  pages        = {1230--1240},
  title        = {Molecular characterization of plant ubiquitin-conjugating enzymes belonging to the UbcP4/E2-C/UBCx/UbcH10 gene family},
  url          = {http://dx.doi.org/10.1104/pp.011353},
  volume       = {130},
  year         = {2002},
}

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