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Detection of DNA during the refining of soybean oil.

Nicolas Gryson UGent, Frederik Ronsse UGent, Kathy Messens UGent, Marc De Loose UGent, Tom VERLEYEN and Koen Dewettinck UGent (2002) JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY. 79(2). p.171-174
abstract
The isolation of DNA from foodstuffs is the first step in the detection of genetically modified organisms. Refining processes, however, have an irrevocable influence on the quality and quantity of DNA and make detection in refined oil impossible. In order to determine the most significant step in removing DNA from crude soybean oil, two refining processes were considered: chemical refining and physical refining. Although conducted on a lab scale, quality parameters showed that the refining processes were good simulations of the industrial refining. From samples drawn at various refining stages, DNA was extracted with a protocol originally developed for the extraction of DNA from lecithin. The polymerase chain reaction results prove that the protocol was sufficiently useful for extracting DNA from soybean oil. The amplified DNA revealed that degumming is the most important step in removing DNA from crude soybean oil. After degumming, DNA was concentrated in the water fraction; no DNA could be amplified in the oil fractions. During physical degumming, degradation of DNA was observed.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
BEANS, FATE, PRODUCTS, PCR, BT MAIZE, FOOD SAMPLES, POLYMERASE-CHAIN-REACTION, GENETICALLY-MODIFIED SOYA, soybean oil, refining, detection limit, DNA, degumming
journal title
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
J. Am. Oil Chem. Soc.
volume
79
issue
2
pages
171-174 pages
Web of Science type
Article
Web of Science id
000174062700011
JCR category
FOOD SCIENCE & TECHNOLOGY
JCR impact factor
1.178 (2002)
JCR rank
22/92 (2002)
JCR quartile
1 (2002)
ISSN
0003-021X
language
English
UGent publication?
yes
classification
A1
id
152104
handle
http://hdl.handle.net/1854/LU-152104
date created
2004-01-14 13:38:00
date last changed
2015-06-17 09:44:07
@article{152104,
  abstract     = {The isolation of DNA from foodstuffs is the first step in the detection of genetically modified organisms. Refining processes, however, have an irrevocable influence on the quality and quantity of DNA and make detection in refined oil impossible. In order to determine the most significant step in removing DNA from crude soybean oil, two refining processes were considered: chemical refining and physical refining. Although conducted on a lab scale, quality parameters showed that the refining processes were good simulations of the industrial refining. From samples drawn at various refining stages, DNA was extracted with a protocol originally developed for the extraction of DNA from lecithin. The polymerase chain reaction results prove that the protocol was sufficiently useful for extracting DNA from soybean oil. The amplified DNA revealed that degumming is the most important step in removing DNA from crude soybean oil. After degumming, DNA was concentrated in the water fraction; no DNA could be amplified in the oil fractions. During physical degumming, degradation of DNA was observed.},
  author       = {Gryson, Nicolas and Ronsse, Frederik and Messens, Kathy and De Loose, Marc and VERLEYEN, Tom and Dewettinck, Koen},
  issn         = {0003-021X},
  journal      = {JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY},
  keyword      = {BEANS,FATE,PRODUCTS,PCR,BT MAIZE,FOOD SAMPLES,POLYMERASE-CHAIN-REACTION,GENETICALLY-MODIFIED SOYA,soybean oil,refining,detection limit,DNA,degumming},
  language     = {eng},
  number       = {2},
  pages        = {171--174},
  title        = {Detection of DNA during the refining of soybean oil.},
  volume       = {79},
  year         = {2002},
}

Chicago
Gryson, Nicolas, Frederik Ronsse, Kathy Messens, Marc De Loose, Tom VERLEYEN, and Koen Dewettinck. 2002. “Detection of DNA During the Refining of Soybean Oil.” Journal of the American Oil Chemists Society 79 (2): 171–174.
APA
Gryson, Nicolas, Ronsse, F., Messens, K., De Loose, M., VERLEYEN, T., & Dewettinck, K. (2002). Detection of DNA during the refining of soybean oil. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 79(2), 171–174.
Vancouver
1.
Gryson N, Ronsse F, Messens K, De Loose M, VERLEYEN T, Dewettinck K. Detection of DNA during the refining of soybean oil. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY. 2002;79(2):171–4.
MLA
Gryson, Nicolas, Frederik Ronsse, Kathy Messens, et al. “Detection of DNA During the Refining of Soybean Oil.” JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY 79.2 (2002): 171–174. Print.