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α-Catenin-vinculin interaction functions to organize the apical junctional complex in epithelial cells

Mitsuko Watabe-Uchida, Naoshige Uchida, Yuzo Imamura, Akira Nagafuchi, Kazushi Fujimoto, Tadashi Uemura, Stefan Vermeulen UGent, Frans Van Roy UGent, Eileen D Adamson and Masatoshi Takeichi (1998) JOURNAL OF CELL BIOLOGY. 142(3). p.847-857
abstract
alpha E-catenin, a cadherin-associated protein, is required for tight junction (TJ) organization, but its role is poorly understood. We transfected an alpha E-catenin-deficient colon carcinoma line with a series of alpha E-catenin mutant constructs. The results showed that the amino acid 326-509 domain of this catenin was required to organize TJs, and its COOH-terminal domain was not essential for this process. The 326-509 internal domain was found to bind vinculin. When an NH2-terminal alpha E-catenin fragment, which is by itself unable to organize the TJ, was fused with the vinculin tail, this chimeric molecule could induce TJ assembly in the alpha E-catenin-deficient cells. In vinculin-null F9 cells, their apical junctional organization was impaired, and this phenotype was rescued by reexpression of vinculin. These results indicate that the alpha E-catenin-vinculin interaction plays a role in the assembly of the apical junctional complex in epithelia.
Please use this url to cite or link to this publication:
author
organization
alternative title
alpha-Catenin-vinculin interaction functions to organize the apical junctional complex in epithelial cells
year
type
journalArticle (original)
publication status
published
subject
keyword
cadherin, catenin, tight junction, vinculin, zonula adherens, ADHESION MOLECULE UVOMORULIN, CADHERIN-ASSOCIATED PROTEIN, TIGHT JUNCTIONS, ACTIN-BINDING, CARCINOMA-CELLS, F-ACTIN, INTRAMOLECULAR ASSOCIATION, IMMUNOELECTRON MICROSCOPE, ADHERENS JUNCTION, BRUSH-BORDER
journal title
JOURNAL OF CELL BIOLOGY
J. Cell Biol.
volume
142
issue
3
pages
847 - 857
Web of Science type
Article
Web of Science id
000075415700020
ISSN
0021-9525
DOI
10.1083/jcb.142.3.847
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
151543
handle
http://hdl.handle.net/1854/LU-151543
date created
2004-01-14 13:38:00
date last changed
2018-06-21 09:01:57
@article{151543,
  abstract     = {alpha E-catenin, a cadherin-associated protein, is required for tight junction (TJ) organization, but its role is poorly understood. We transfected an alpha E-catenin-deficient colon carcinoma line with a series of alpha E-catenin mutant constructs. The results showed that the amino acid 326-509 domain of this catenin was required to organize TJs, and its COOH-terminal domain was not essential for this process. The 326-509 internal domain was found to bind vinculin. When an NH2-terminal alpha E-catenin fragment, which is by itself unable to organize the TJ, was fused with the vinculin tail, this chimeric molecule could induce TJ assembly in the alpha E-catenin-deficient cells. In vinculin-null F9 cells, their apical junctional organization was impaired, and this phenotype was rescued by reexpression of vinculin. These results indicate that the alpha E-catenin-vinculin interaction plays a role in the assembly of the apical junctional complex in epithelia.},
  author       = {Watabe-Uchida, Mitsuko and Uchida, Naoshige and Imamura, Yuzo and Nagafuchi, Akira and Fujimoto, Kazushi and Uemura, Tadashi and Vermeulen, Stefan and Van Roy, Frans and Adamson, Eileen D and Takeichi, Masatoshi},
  issn         = {0021-9525},
  journal      = {JOURNAL OF CELL BIOLOGY},
  keyword      = {cadherin,catenin,tight junction,vinculin,zonula adherens,ADHESION MOLECULE UVOMORULIN,CADHERIN-ASSOCIATED PROTEIN,TIGHT JUNCTIONS,ACTIN-BINDING,CARCINOMA-CELLS,F-ACTIN,INTRAMOLECULAR ASSOCIATION,IMMUNOELECTRON MICROSCOPE,ADHERENS JUNCTION,BRUSH-BORDER},
  language     = {eng},
  number       = {3},
  pages        = {847--857},
  title        = {\ensuremath{\alpha}-Catenin-vinculin interaction functions to organize the apical junctional complex in epithelial cells},
  url          = {http://dx.doi.org/10.1083/jcb.142.3.847},
  volume       = {142},
  year         = {1998},
}

Chicago
Watabe-Uchida, Mitsuko, Naoshige Uchida, Yuzo Imamura, Akira Nagafuchi, Kazushi Fujimoto, Tadashi Uemura, Stefan Vermeulen, Frans Van Roy, Eileen D Adamson, and Masatoshi Takeichi. 1998. “α-Catenin-vinculin Interaction Functions to Organize the Apical Junctional Complex in Epithelial Cells.” Journal of Cell Biology 142 (3): 847–857.
APA
Watabe-Uchida, M., Uchida, N., Imamura, Y., Nagafuchi, A., Fujimoto, K., Uemura, T., Vermeulen, S., et al. (1998). α-Catenin-vinculin interaction functions to organize the apical junctional complex in epithelial cells. JOURNAL OF CELL BIOLOGY, 142(3), 847–857.
Vancouver
1.
Watabe-Uchida M, Uchida N, Imamura Y, Nagafuchi A, Fujimoto K, Uemura T, et al. α-Catenin-vinculin interaction functions to organize the apical junctional complex in epithelial cells. JOURNAL OF CELL BIOLOGY. 1998;142(3):847–57.
MLA
Watabe-Uchida, Mitsuko, Naoshige Uchida, Yuzo Imamura, et al. “α-Catenin-vinculin Interaction Functions to Organize the Apical Junctional Complex in Epithelial Cells.” JOURNAL OF CELL BIOLOGY 142.3 (1998): 847–857. Print.