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α-Catenin-vinculin interaction functions to organize the apical junctional complex in epithelial cells

(1998) JOURNAL OF CELL BIOLOGY. 142(3). p.847-857
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Organization
Abstract
alpha E-catenin, a cadherin-associated protein, is required for tight junction (TJ) organization, but its role is poorly understood. We transfected an alpha E-catenin-deficient colon carcinoma line with a series of alpha E-catenin mutant constructs. The results showed that the amino acid 326-509 domain of this catenin was required to organize TJs, and its COOH-terminal domain was not essential for this process. The 326-509 internal domain was found to bind vinculin. When an NH2-terminal alpha E-catenin fragment, which is by itself unable to organize the TJ, was fused with the vinculin tail, this chimeric molecule could induce TJ assembly in the alpha E-catenin-deficient cells. In vinculin-null F9 cells, their apical junctional organization was impaired, and this phenotype was rescued by reexpression of vinculin. These results indicate that the alpha E-catenin-vinculin interaction plays a role in the assembly of the apical junctional complex in epithelia.
Keywords
cadherin, catenin, tight junction, vinculin, zonula adherens, ADHESION MOLECULE UVOMORULIN, CADHERIN-ASSOCIATED PROTEIN, TIGHT JUNCTIONS, ACTIN-BINDING, CARCINOMA-CELLS, F-ACTIN, INTRAMOLECULAR ASSOCIATION, IMMUNOELECTRON MICROSCOPE, ADHERENS JUNCTION, BRUSH-BORDER

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Citation

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Chicago
Watabe-Uchida, Mitsuko, Naoshige Uchida, Yuzo Imamura, Akira Nagafuchi, Kazushi Fujimoto, Tadashi Uemura, Stefan Vermeulen, Frans Van Roy, Eileen D Adamson, and Masatoshi Takeichi. 1998. “α-Catenin-vinculin Interaction Functions to Organize the Apical Junctional Complex in Epithelial Cells.” Journal of Cell Biology 142 (3): 847–857.
APA
Watabe-Uchida, M., Uchida, N., Imamura, Y., Nagafuchi, A., Fujimoto, K., Uemura, T., Vermeulen, S., et al. (1998). α-Catenin-vinculin interaction functions to organize the apical junctional complex in epithelial cells. JOURNAL OF CELL BIOLOGY, 142(3), 847–857.
Vancouver
1.
Watabe-Uchida M, Uchida N, Imamura Y, Nagafuchi A, Fujimoto K, Uemura T, et al. α-Catenin-vinculin interaction functions to organize the apical junctional complex in epithelial cells. JOURNAL OF CELL BIOLOGY. 1998;142(3):847–57.
MLA
Watabe-Uchida, Mitsuko, Naoshige Uchida, Yuzo Imamura, et al. “α-Catenin-vinculin Interaction Functions to Organize the Apical Junctional Complex in Epithelial Cells.” JOURNAL OF CELL BIOLOGY 142.3 (1998): 847–857. Print.
@article{151543,
  abstract     = {alpha E-catenin, a cadherin-associated protein, is required for tight junction (TJ) organization, but its role is poorly understood. We transfected an alpha E-catenin-deficient colon carcinoma line with a series of alpha E-catenin mutant constructs. The results showed that the amino acid 326-509 domain of this catenin was required to organize TJs, and its COOH-terminal domain was not essential for this process. The 326-509 internal domain was found to bind vinculin. When an NH2-terminal alpha E-catenin fragment, which is by itself unable to organize the TJ, was fused with the vinculin tail, this chimeric molecule could induce TJ assembly in the alpha E-catenin-deficient cells. In vinculin-null F9 cells, their apical junctional organization was impaired, and this phenotype was rescued by reexpression of vinculin. These results indicate that the alpha E-catenin-vinculin interaction plays a role in the assembly of the apical junctional complex in epithelia.},
  author       = {Watabe-Uchida, Mitsuko and Uchida, Naoshige and Imamura, Yuzo and Nagafuchi, Akira and Fujimoto, Kazushi and Uemura, Tadashi and Vermeulen, Stefan and Van Roy, Frans and Adamson, Eileen D and Takeichi, Masatoshi},
  issn         = {0021-9525},
  journal      = {JOURNAL OF CELL BIOLOGY},
  keyword      = {cadherin,catenin,tight junction,vinculin,zonula adherens,ADHESION MOLECULE UVOMORULIN,CADHERIN-ASSOCIATED PROTEIN,TIGHT JUNCTIONS,ACTIN-BINDING,CARCINOMA-CELLS,F-ACTIN,INTRAMOLECULAR ASSOCIATION,IMMUNOELECTRON MICROSCOPE,ADHERENS JUNCTION,BRUSH-BORDER},
  language     = {eng},
  number       = {3},
  pages        = {847--857},
  title        = {\ensuremath{\alpha}-Catenin-vinculin interaction functions to organize the apical junctional complex in epithelial cells},
  url          = {http://dx.doi.org/10.1083/jcb.142.3.847},
  volume       = {142},
  year         = {1998},
}

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