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FGF-3 and FGF-4 elicit distinct oncogenic properties in mouse mammary myoepithelial cells

(1998) ONCOGENE. 17(16). p.2059-2071
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Abstract
Fibroblast Growth Factors 3 (FGF-3) and 4 (FGF-4) mere compared for the effects they each exert on EF43 mouse cells. This non-transformed mammary cell line appears to be myoepithelial mainly because it expresses cc-smooth muscle actin, The EF43 cells were infected with similar vectors that carry either the short fgf-3 sequence (the product of which goes into the secretory pathway), fgf-4 or the selection gene only as control, In syngeneic animals, EF43,fgf-3 cells were tumorigenic only when orthotopically implanted whereas EF43.fgf-4 cells invariably gave rise to aggressive tumors. However, both tumor types were metastatic as evidenced by the blue micrometastases observed when the implanted cells expressed lacZ. In vitro, the FGF-3 producing cells were strongly invasive in matrigel coated chambers whereas the EF43.fgf-4 cells only were invasive in type I-collagen gels, Interestingly, FGF-3 production greatly stimulated the synthesis of pro-MMP-9 (Matrix Metalloprotease-9) and, to a lesser extent, that of pro-MMP-2, FGF-3 also up-regulated the production of plasminogen activators. In contrast, FGF-4 had no effect on these secretions and the medium conditioned by the EF43.fgf-4 cells displayed the largest plasminogen activator-inhibitor activity, These results show that FGF-3 and FGF-4 have distinct mechanisms of action on myoepithelial cells.
Keywords
FGF-3, FGF-4, mammary tumors, myoepithelial cells, FIBROBLAST GROWTH-FACTOR, E-CADHERIN EXPRESSION, SMOOTH-MUSCLE ACTIN, HUMAN BREAST-CANCER, TRANSGENIC MICE, TUMOR VIRUS, PROVIRAL INSERTION, FACTOR FAMILY, NUDE-MICE, IN-VITRO

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Chicago
Hajitou, A, EN Baramova, K Bajou, V Noe, Erik Bruyneel, Marcus Mareel, J Collette, JM Foidart, and CM Calberg-Bacq. 1998. “FGF-3 and FGF-4 Elicit Distinct Oncogenic Properties in Mouse Mammary Myoepithelial Cells.” Oncogene 17 (16): 2059–2071.
APA
Hajitou, A., Baramova, E., Bajou, K., Noe, V., Bruyneel, E., Mareel, M., Collette, J., et al. (1998). FGF-3 and FGF-4 elicit distinct oncogenic properties in mouse mammary myoepithelial cells. ONCOGENE, 17(16), 2059–2071.
Vancouver
1.
Hajitou A, Baramova E, Bajou K, Noe V, Bruyneel E, Mareel M, et al. FGF-3 and FGF-4 elicit distinct oncogenic properties in mouse mammary myoepithelial cells. ONCOGENE. 1998;17(16):2059–71.
MLA
Hajitou, A, EN Baramova, K Bajou, et al. “FGF-3 and FGF-4 Elicit Distinct Oncogenic Properties in Mouse Mammary Myoepithelial Cells.” ONCOGENE 17.16 (1998): 2059–2071. Print.
@article{151524,
  abstract     = {Fibroblast Growth Factors 3 (FGF-3) and 4 (FGF-4) mere compared for the effects they each exert on EF43 mouse cells. This non-transformed mammary cell line appears to be myoepithelial mainly because it expresses cc-smooth muscle actin, The EF43 cells were infected with similar vectors that carry either the short fgf-3 sequence (the product of which goes into the secretory pathway), fgf-4 or the selection gene only as control, In syngeneic animals, EF43,fgf-3 cells were tumorigenic only when orthotopically implanted whereas EF43.fgf-4 cells invariably gave rise to aggressive tumors. However, both tumor types were metastatic as evidenced by the blue micrometastases observed when the implanted cells expressed lacZ. In vitro, the FGF-3 producing cells were strongly invasive in matrigel coated chambers whereas the EF43.fgf-4 cells only were invasive in type I-collagen gels, Interestingly, FGF-3 production greatly stimulated the synthesis of pro-MMP-9 (Matrix Metalloprotease-9) and, to a lesser extent, that of pro-MMP-2, FGF-3 also up-regulated the production of plasminogen activators. In contrast, FGF-4 had no effect on these secretions and the medium conditioned by the EF43.fgf-4 cells displayed the largest plasminogen activator-inhibitor activity, These results show that FGF-3 and FGF-4 have distinct mechanisms of action on myoepithelial cells.},
  author       = {Hajitou, A and Baramova, EN and Bajou, K and Noe, V and Bruyneel, Erik and Mareel, Marcus and Collette, J and Foidart, JM and Calberg-Bacq, CM},
  issn         = {0950-9232},
  journal      = {ONCOGENE},
  keyword      = {FGF-3,FGF-4,mammary tumors,myoepithelial cells,FIBROBLAST GROWTH-FACTOR,E-CADHERIN EXPRESSION,SMOOTH-MUSCLE ACTIN,HUMAN BREAST-CANCER,TRANSGENIC MICE,TUMOR VIRUS,PROVIRAL INSERTION,FACTOR FAMILY,NUDE-MICE,IN-VITRO},
  language     = {eng},
  number       = {16},
  pages        = {2059--2071},
  title        = {FGF-3 and FGF-4 elicit distinct oncogenic properties in mouse mammary myoepithelial cells},
  url          = {http://dx.doi.org/10.1038/sj.onc.1202126},
  volume       = {17},
  year         = {1998},
}

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