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Interleukin-1 is a motility factor for human breast carcinoma cells in vitro : additive effect with interleukin-6

Bruno Verhasselt (UGent) , Jozef Van Damme (UGent) , Nicolas Van Larebeke (UGent) , W Put, Marc Bracke (UGent) , C De Potter and Marcus Mareel (UGent)
Author
Organization
Abstract
Interleukin-1beta (Il-1beta) and interleukin-1alpha (Il-1alpha) were shown to act as motility factors for the human breast carcinoma cell lines SK-BR-3 and ZR-75-1 in vitro. Both cytokines induced transition from the stationary to the motile phenotype (spreading). Il-1beta stimulated translocation, shape change and random migration (chemokinesis) of SK-BR-3 cells as demonstrated by time-lapse video recordings and by a modified Boyden chamber assay. Interleukin-6 (Il-6) stimulated spreading of the SK-BR-3 cells; an additive effect with Il-1beta on spreading and fast plasma membrane movements was evidenced. In the SK-BR-3 cell line, the signal transduction of Il-1beta and Il-6 differed, since only the effect of Il-6 on spreading was sensitive to pertussis toxin. Both Il-1beta and Il-6 required protein synthesis to stimulate spreading, since cycloheximide inhibited the effect of the cytokines. Induction of an autocrine loop of Il-6 in the SK-BR-3 cells by Il-1beta was unlikely, since after stimulation with Il-1beta, no induction of Il-6 activity was measured, nor was inhibition of stimulated spreading seen in the presence of an antiserum against Il-6. Addition of Il-8 or of an antiserum against Il-8 did not affect spreading. We concluded that Il-1 and Il-6 could act as motility factors for human breast carcinoma cells, in both an independent and an additive way. Human squamous cell carcinoma COLO-16 cells were found to secrete factors with similar effects on the SK-BR-3 and ZR-75-1 cells as the aforementioned cytokines. Although the presence of Il-1alpha and Il-6 activity in the COLO-16 conditioned medium was confirmed, a number of arguments were found to assume that still other factors beside these two cytokines were responsible for the motility-stimulating effect of the COLO-16 conditioned medium.
Keywords
INTERLEUKIN-6, INTERLEUKIN-1, MOTILITY, HUMAN BREAST CARCINOMA CELLS, CYTOKINE INTERACTION, TUMOR NECROSIS FACTOR, GROWTH-FACTOR, HUMAN-MELANOMA, FIBROBLASTS, INTERFERON-BETA-2, IDENTIFICATION, PURIFICATION, EXPRESSION, MOVEMENT, SEQUENCE

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Chicago
Verhasselt, Bruno, Jozef Van Damme, Nicolas Van Larebeke, W Put, Marc Bracke, C De Potter, and Marcus Mareel. 1992. “Interleukin-1 Is a Motility Factor for Human Breast Carcinoma Cells in Vitro : Additive Effect with Interleukin-6.” European Journal of Cell Biology 59 (2): 449–457.
APA
Verhasselt, B., Van Damme, J., Van Larebeke, N., Put, W., Bracke, M., De Potter, C., & Mareel, M. (1992). Interleukin-1 is a motility factor for human breast carcinoma cells in vitro : additive effect with interleukin-6. EUROPEAN JOURNAL OF CELL BIOLOGY, 59(2), 449–457.
Vancouver
1.
Verhasselt B, Van Damme J, Van Larebeke N, Put W, Bracke M, De Potter C, et al. Interleukin-1 is a motility factor for human breast carcinoma cells in vitro : additive effect with interleukin-6. EUROPEAN JOURNAL OF CELL BIOLOGY. 1992;59(2):449–57.
MLA
Verhasselt, Bruno, Jozef Van Damme, Nicolas Van Larebeke, et al. “Interleukin-1 Is a Motility Factor for Human Breast Carcinoma Cells in Vitro : Additive Effect with Interleukin-6.” EUROPEAN JOURNAL OF CELL BIOLOGY 59.2 (1992): 449–457. Print.
@article{151216,
  abstract     = {Interleukin-1beta (Il-1beta) and interleukin-1alpha (Il-1alpha) were shown to act as motility factors for the human breast carcinoma cell lines SK-BR-3 and ZR-75-1 in vitro. Both cytokines induced transition from the stationary to the motile phenotype (spreading). Il-1beta stimulated translocation, shape change and random migration (chemokinesis) of SK-BR-3 cells as demonstrated by time-lapse video recordings and by a modified Boyden chamber assay. Interleukin-6 (Il-6) stimulated spreading of the SK-BR-3 cells; an additive effect with Il-1beta on spreading and fast plasma membrane movements was evidenced. In the SK-BR-3 cell line, the signal transduction of Il-1beta and Il-6 differed, since only the effect of Il-6 on spreading was sensitive to pertussis toxin. Both Il-1beta and Il-6 required protein synthesis to stimulate spreading, since cycloheximide inhibited the effect of the cytokines. Induction of an autocrine loop of Il-6 in the SK-BR-3 cells by Il-1beta was unlikely, since after stimulation with Il-1beta, no induction of Il-6 activity was measured, nor was inhibition of stimulated spreading seen in the presence of an antiserum against Il-6. Addition of Il-8 or of an antiserum against Il-8 did not affect spreading. We concluded that Il-1 and Il-6 could act as motility factors for human breast carcinoma cells, in both an independent and an additive way. Human squamous cell carcinoma COLO-16 cells were found to secrete factors with similar effects on the SK-BR-3 and ZR-75-1 cells as the aforementioned cytokines. Although the presence of Il-1alpha and Il-6 activity in the COLO-16 conditioned medium was confirmed, a number of arguments were found to assume that still other factors beside these two cytokines were responsible for the motility-stimulating effect of the COLO-16 conditioned medium.},
  author       = {Verhasselt, Bruno and Van Damme, Jozef and Van Larebeke, Nicolas and Put, W and Bracke, Marc and De Potter, C and Mareel, Marcus},
  issn         = {0171-9335},
  journal      = {EUROPEAN JOURNAL OF CELL BIOLOGY},
  keyword      = {INTERLEUKIN-6,INTERLEUKIN-1,MOTILITY,HUMAN BREAST CARCINOMA CELLS,CYTOKINE INTERACTION,TUMOR NECROSIS FACTOR,GROWTH-FACTOR,HUMAN-MELANOMA,FIBROBLASTS,INTERFERON-BETA-2,IDENTIFICATION,PURIFICATION,EXPRESSION,MOVEMENT,SEQUENCE},
  language     = {eng},
  number       = {2},
  pages        = {449--457},
  title        = {Interleukin-1 is a motility factor for human breast carcinoma cells in vitro : additive effect with interleukin-6},
  volume       = {59},
  year         = {1992},
}