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Arrest of MCF-7 cell migration by laminin in vitro: possible mechanisms

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Abstract
Laminin, a major basement membrane component, arrested the migration of MCF-7/AZ human breast adenocarcinoma cells that were not invasive in vitro. Migration of invasive MCF-7/6 cells was not affected by laminin. Both cell types expressed the 67 kD laminin receptor, at both mRNA and protein level, but did not express the alpha-6 subunit of the VLA-6 integrin-type laminin receptor. The presence of YIGSR peptides (100-mu-g/ml), reported to block the interaction between laminin and its 67 kD receptor, did not change the migratory response of MCF-7/AZ or MCF-7/6 cells when meeting laminin lanes. In addition, the migration of these cell types was not affected by the presence of 17-beta-estradiol (10(-6) M) or all-trans retinoic acid (10(-6) M), which were both reported to increase the number of 67 kD receptors. We could therefore not assign an involvement of the 67 kD receptors in migration of MCF-7 cells on laminin, nor did we find evidence that conditioned medium of MCF-7/6 cells contains factors that are able to initiate migration of MCF-7/AZ cells on laminin.
Keywords
EXTRACELLULAR-MATRIX, RECEPTOR MESSENGER-RNA, BASEMENT-MEMBRANE, CANCER-CELLS, EXPRESSION, ATTACHMENT, CARCINOMA, LINE, GLYCOPROTEIN, PENTAPEPTIDE

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Chicago
Coopman, P, Bruno Verhasselt, Marc Bracke, Georges De Bruyne, V Castronovo, M Sobel, JM Foidart, Frans Van Roy, and Marcus Mareel. 1991. “Arrest of MCF-7 Cell Migration by Laminin in Vitro: Possible Mechanisms.” Clinical & Experimental Metastasis 9 (5): 469–484.
APA
Coopman, P, Verhasselt, B., Bracke, M., De Bruyne, G., Castronovo, V., Sobel, M., Foidart, J., et al. (1991). Arrest of MCF-7 cell migration by laminin in vitro: possible mechanisms. CLINICAL & EXPERIMENTAL METASTASIS, 9(5), 469–484.
Vancouver
1.
Coopman P, Verhasselt B, Bracke M, De Bruyne G, Castronovo V, Sobel M, et al. Arrest of MCF-7 cell migration by laminin in vitro: possible mechanisms. CLINICAL & EXPERIMENTAL METASTASIS. 1991;9(5):469–84.
MLA
Coopman, P, Bruno Verhasselt, Marc Bracke, et al. “Arrest of MCF-7 Cell Migration by Laminin in Vitro: Possible Mechanisms.” CLINICAL & EXPERIMENTAL METASTASIS 9.5 (1991): 469–484. Print.
@article{151145,
  abstract     = {Laminin, a major basement membrane component, arrested the migration of MCF-7/AZ human breast adenocarcinoma cells that were not invasive in vitro. Migration of invasive MCF-7/6 cells was not affected by laminin. Both cell types expressed the 67 kD laminin receptor, at both mRNA and protein level, but did not express the alpha-6 subunit of the VLA-6 integrin-type laminin receptor. The presence of YIGSR peptides (100-mu-g/ml), reported to block the interaction between laminin and its 67 kD receptor, did not change the migratory response of MCF-7/AZ or MCF-7/6 cells when meeting laminin lanes. In addition, the migration of these cell types was not affected by the presence of 17-beta-estradiol (10(-6) M) or all-trans retinoic acid (10(-6) M), which were both reported to increase the number of 67 kD receptors. We could therefore not assign an involvement of the 67 kD receptors in migration of MCF-7 cells on laminin, nor did we find evidence that conditioned medium of MCF-7/6 cells contains factors that are able to initiate migration of MCF-7/AZ cells on laminin.},
  author       = {Coopman, P and Verhasselt, Bruno and Bracke, Marc and De Bruyne, Georges and Castronovo, V and Sobel, M and Foidart, JM and Van Roy, Frans and Mareel, Marcus},
  issn         = {0262-0898},
  journal      = {CLINICAL \& EXPERIMENTAL METASTASIS},
  keyword      = {EXTRACELLULAR-MATRIX,RECEPTOR MESSENGER-RNA,BASEMENT-MEMBRANE,CANCER-CELLS,EXPRESSION,ATTACHMENT,CARCINOMA,LINE,GLYCOPROTEIN,PENTAPEPTIDE},
  language     = {eng},
  number       = {5},
  pages        = {469--484},
  title        = {Arrest of MCF-7 cell migration by laminin in vitro: possible mechanisms},
  url          = {http://dx.doi.org/10.1007/BF01785532},
  volume       = {9},
  year         = {1991},
}

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