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Modulation of the peroxisomal gene expression pattern by dehydroepiandrosterone and vitamin D : therapeutic implications

Marianne Depreter, Jo Vandesompele (UGent) , Marc Espeel (UGent) , Franki Speleman (UGent) and Frank Roels (UGent)
(2002) JOURNAL OF ENDOCRINOLOGY. 175(3). p.779-792
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Abstract
Peroxisomes are ubiquitous organelles required for several metabolic functions. Their dysfunction is responsible for a group of human inherited disorders. In the search for endogenous factors regulating the peroxisomal compartment in normal liver, we treated female rats with dehydroepiandrosterone (DHEA) and 25-hydroxy-cholecalciferol for 1 and 6 days. Relative transcription levels of 39 selected genes were evaluated by real-time quantitative RT-PCR analysis. Catalase (peroxisomal marker)-specific activity was assayed in total liver homogenate and peroxisomes were visualized by catalase localization. DHEA induced peroxisome proliferation and raised catalase specific activity. Expression levels of 16 (of which 11 were peroxisomal) genes were altered. Pex 11, acyl-CoA oxidase, L- and D-multifunctional enzyme, thiolase 1, phytanoyl-CoA hydroxylase, 70 kDa peroxisomal membrane protein and very long chain acyl-CoA synthetase were upregulated, three others were downregulated. Vitamin D caused downregulation of six genes. Administration of vitamin D to peroxisomal disorder patients may be contraindicated. The adrenocortical hormone DHEA is a potential natural regulator of the peroxisomal compartment. Its therapeutic use in X-linked adrenoleukodystrophy, some other beta-oxidation defects and classical Refsum should be considered.
Keywords
ACTIVATED RECEPTOR-ALPHA, X-LINKED ADRENOLEUKODYSTROPHY, CHAIN FATTY-ACIDS, ACYL-COA OXIDASE, RAT-LIVER, MEMBRANE-PROTEIN, BETA-OXIDATION, SACCHAROMYCES-CEREVISIAE, HYPOLIPIDEMIC AGENT, NUCLEAR RECEPTORS

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Chicago
Depreter, Marianne, Jo Vandesompele, Marc Espeel, Franki Speleman, and Frank Roels. 2002. “Modulation of the Peroxisomal Gene Expression Pattern by Dehydroepiandrosterone and Vitamin D : Therapeutic Implications.” Journal of Endocrinology 175 (3): 779–792.
APA
Depreter, M., Vandesompele, J., Espeel, M., Speleman, F., & Roels, F. (2002). Modulation of the peroxisomal gene expression pattern by dehydroepiandrosterone and vitamin D : therapeutic implications. JOURNAL OF ENDOCRINOLOGY, 175(3), 779–792.
Vancouver
1.
Depreter M, Vandesompele J, Espeel M, Speleman F, Roels F. Modulation of the peroxisomal gene expression pattern by dehydroepiandrosterone and vitamin D : therapeutic implications. JOURNAL OF ENDOCRINOLOGY. 2002;175(3):779–92.
MLA
Depreter, Marianne et al. “Modulation of the Peroxisomal Gene Expression Pattern by Dehydroepiandrosterone and Vitamin D : Therapeutic Implications.” JOURNAL OF ENDOCRINOLOGY 175.3 (2002): 779–792. Print.
@article{150138,
  abstract     = {Peroxisomes are ubiquitous organelles required for several metabolic functions. Their dysfunction is responsible for a group of human inherited disorders. In the search for endogenous factors regulating the peroxisomal compartment in normal liver, we treated female rats with dehydroepiandrosterone (DHEA) and 25-hydroxy-cholecalciferol for 1 and 6 days. Relative transcription levels of 39 selected genes were evaluated by real-time quantitative RT-PCR analysis. Catalase (peroxisomal marker)-specific activity was assayed in total liver homogenate and peroxisomes were visualized by catalase localization. DHEA induced peroxisome proliferation and raised catalase specific activity. Expression levels of 16 (of which 11 were peroxisomal) genes were altered. Pex 11, acyl-CoA oxidase, L- and D-multifunctional enzyme, thiolase 1, phytanoyl-CoA hydroxylase, 70 kDa peroxisomal membrane protein and very long chain acyl-CoA synthetase were upregulated, three others were downregulated. Vitamin D caused downregulation of six genes. Administration of vitamin D to peroxisomal disorder patients may be contraindicated. The adrenocortical hormone DHEA is a potential natural regulator of the peroxisomal compartment. Its therapeutic use in X-linked adrenoleukodystrophy, some other beta-oxidation defects and classical Refsum should be considered.},
  author       = {Depreter, Marianne and Vandesompele, Jo and Espeel, Marc and Speleman, Franki and Roels, Frank},
  issn         = {0022-0795},
  journal      = {JOURNAL OF ENDOCRINOLOGY},
  keywords     = {ACTIVATED RECEPTOR-ALPHA,X-LINKED ADRENOLEUKODYSTROPHY,CHAIN FATTY-ACIDS,ACYL-COA OXIDASE,RAT-LIVER,MEMBRANE-PROTEIN,BETA-OXIDATION,SACCHAROMYCES-CEREVISIAE,HYPOLIPIDEMIC AGENT,NUCLEAR RECEPTORS},
  language     = {eng},
  number       = {3},
  pages        = {779--792},
  title        = {Modulation of the peroxisomal gene expression pattern by dehydroepiandrosterone and vitamin D : therapeutic implications},
  url          = {http://dx.doi.org/10.1677/joe.0.1750779},
  volume       = {175},
  year         = {2002},
}

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