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Inositol 1,4,5-trisphosphate receptor function in human oocytes: calcium responses and oocyte activation-related phenomena induced by photolytic release of InsP(3) are blocked by a specific antibody to the type I receptor

Pravin T Goud, Anuradha P Goud, Luc Leybaert UGent, Patric Van Oostveldt UGent, K Mikoshiba, Michael P Diamond and Marc Dhont (2002) MOLECULAR HUMAN REPRODUCTION. 8(10). p.912-918
abstract
Tape I inositol 1,4,5-trisphosphate-sensitive receptors (InsP(3)R) are expressed in human oocytes and may be involved in operating the Ca2+ release triggered by the fertilizing sperm. This study examines the contribution of type I InsP(3)R in operating Ca2+ release in human oocytes secondary to InsP(3) itself, using a specific function-blocking antibody in conjunction with photolytic release of microinjected InsP(3). Intracellular Ca2+ responses were assessed in oocytes microinjected with only caged InsP(3) in experiment set A, while in experiment sets B and C, sibling oocytes were injected with caged InsP(3) and the blocking antibody or a corresponding volume of medium, prior to flash photolysis. In experiment set C, certain fertilization-related phenomena (cortical granule exocytosis and chromatin configurations) were assessed using optical sections and three-dimensional image reconstructions obtained from a confocal laser scanning microscope. In experiment set A, photolytic release of InsP(3) triggered a Ca2+ response (increase from similar to100 to 220 nmol/l followed by an exponential recovery, n = 8) and a wave in the oocytes that spread from the stimulation point to the opposite pole. In set B, photolytic InsP(3) release generated Ca2+ responses in control oocytes (n = 9), but not in the antibody-injected oocytes (n = 7). In set C, cortical granule exocytosis and anaphase chromosome configurations were noted in the control oocytes after flash photolysis (n = 6). These changes were completely absent in antibody injected oocytes as their cortical granules were intact and the chromosomes were in metaphase. These oocytes had also lacked Ca2+ responses as in set B (n = 5). This study demonstrates the functional presence of type I InsP(3)R-operated Ca2+ channels in human oocytes and further suggests an active role of InsP(3) in triggering the Ca2+ rise and secondary activation phenomena at fertilization.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (proceedingsPaper)
publication status
published
subject
keyword
MEIOTIC MATURATION, HAMSTER EGGS, ENDOPLASMIC-RETICULUM, VITRO MATURATION, BOVINE OOCYTES, MAMMALIAN EGGS, RYANODINE RECEPTORS, CA2+ RELEASE, MOUSE EGG ACTIVATION, INTRACYTOPLASMIC SPERM INJECTION, 5-trisphosphate receptors, inositol 1, 4, flash photolysis, fertilization, caged IP3, Ca2+ transients
journal title
MOLECULAR HUMAN REPRODUCTION
Mol. Hum. Reprod.
volume
8
issue
10
pages
912 - 918
conference name
56th Annual Meeting of the American-Society-for-Reproductive-Medicine
conference location
San Diego, CA, USA
conference start
2000-10-21
conference end
2000-10-26
Web of Science type
Article
Web of Science id
000178614500005
JCR category
REPRODUCTIVE BIOLOGY
JCR impact factor
2.968 (2002)
JCR rank
6/23 (2002)
JCR quartile
1 (2002)
ISSN
1360-9947
DOI
10.1093/molehr/8.10.912
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
149943
handle
http://hdl.handle.net/1854/LU-149943
date created
2004-01-14 13:38:00
date last changed
2016-12-19 15:38:18
@article{149943,
  abstract     = {Tape I inositol 1,4,5-trisphosphate-sensitive receptors (InsP(3)R) are expressed in human oocytes and may be involved in operating the Ca2+ release triggered by the fertilizing sperm. This study examines the contribution of type I InsP(3)R in operating Ca2+ release in human oocytes secondary to InsP(3) itself, using a specific function-blocking antibody in conjunction with photolytic release of microinjected InsP(3). Intracellular Ca2+ responses were assessed in oocytes microinjected with only caged InsP(3) in experiment set A, while in experiment sets B and C, sibling oocytes were injected with caged InsP(3) and the blocking antibody or a corresponding volume of medium, prior to flash photolysis. In experiment set C, certain fertilization-related phenomena (cortical granule exocytosis and chromatin configurations) were assessed using optical sections and three-dimensional image reconstructions obtained from a confocal laser scanning microscope. In experiment set A, photolytic release of InsP(3) triggered a Ca2+ response (increase from similar to100 to 220 nmol/l followed by an exponential recovery, n = 8) and a wave in the oocytes that spread from the stimulation point to the opposite pole. In set B, photolytic InsP(3) release generated Ca2+ responses in control oocytes (n = 9), but not in the antibody-injected oocytes (n = 7). In set C, cortical granule exocytosis and anaphase chromosome configurations were noted in the control oocytes after flash photolysis (n = 6). These changes were completely absent in antibody injected oocytes as their cortical granules were intact and the chromosomes were in metaphase. These oocytes had also lacked Ca2+ responses as in set B (n = 5). This study demonstrates the functional presence of type I InsP(3)R-operated Ca2+ channels in human oocytes and further suggests an active role of InsP(3) in triggering the Ca2+ rise and secondary activation phenomena at fertilization.},
  author       = {Goud, Pravin T and Goud, Anuradha P and Leybaert, Luc and Van Oostveldt, Patric and Mikoshiba, K and Diamond, Michael P and Dhont, Marc},
  issn         = {1360-9947},
  journal      = {MOLECULAR HUMAN REPRODUCTION},
  keyword      = {MEIOTIC MATURATION,HAMSTER EGGS,ENDOPLASMIC-RETICULUM,VITRO MATURATION,BOVINE OOCYTES,MAMMALIAN EGGS,RYANODINE RECEPTORS,CA2+ RELEASE,MOUSE EGG ACTIVATION,INTRACYTOPLASMIC SPERM INJECTION,5-trisphosphate receptors,inositol 1,4,flash photolysis,fertilization,caged IP3,Ca2+ transients},
  language     = {eng},
  location     = {San Diego, CA, USA},
  number       = {10},
  pages        = {912--918},
  title        = {Inositol 1,4,5-trisphosphate receptor function in human oocytes: calcium responses and oocyte activation-related phenomena induced by photolytic release of InsP(3) are blocked by a specific antibody to the type I receptor},
  url          = {http://dx.doi.org/10.1093/molehr/8.10.912},
  volume       = {8},
  year         = {2002},
}

Chicago
Goud, Pravin T, Anuradha P Goud, Luc Leybaert, Patric Van Oostveldt, K Mikoshiba, Michael P Diamond, and MARC DHONT. 2002. “Inositol 1,4,5-trisphosphate Receptor Function in Human Oocytes: Calcium Responses and Oocyte Activation-related Phenomena Induced by Photolytic Release of InsP(3) Are Blocked by a Specific Antibody to the Type I Receptor.” Molecular Human Reproduction 8 (10): 912–918.
APA
Goud, P. T., Goud, A. P., Leybaert, L., Van Oostveldt, P., Mikoshiba, K., Diamond, M. P., & DHONT, M. (2002). Inositol 1,4,5-trisphosphate receptor function in human oocytes: calcium responses and oocyte activation-related phenomena induced by photolytic release of InsP(3) are blocked by a specific antibody to the type I receptor. MOLECULAR HUMAN REPRODUCTION, 8(10), 912–918. Presented at the 56th Annual Meeting of the American-Society-for-Reproductive-Medicine.
Vancouver
1.
Goud PT, Goud AP, Leybaert L, Van Oostveldt P, Mikoshiba K, Diamond MP, et al. Inositol 1,4,5-trisphosphate receptor function in human oocytes: calcium responses and oocyte activation-related phenomena induced by photolytic release of InsP(3) are blocked by a specific antibody to the type I receptor. MOLECULAR HUMAN REPRODUCTION. 2002;8(10):912–8.
MLA
Goud, Pravin T, Anuradha P Goud, Luc Leybaert, et al. “Inositol 1,4,5-trisphosphate Receptor Function in Human Oocytes: Calcium Responses and Oocyte Activation-related Phenomena Induced by Photolytic Release of InsP(3) Are Blocked by a Specific Antibody to the Type I Receptor.” MOLECULAR HUMAN REPRODUCTION 8.10 (2002): 912–918. Print.