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Effect of enrofloxacin treatment on plasma endotoxin during bovine Escherichia coli mastitis.

Hilde Dosogne, Evelyne Meyer UGent, A STURK, J VAN LOON, AM MASSART-LEEN and Christian Burvenich UGent (2002) INFLAMMATION RESEARCH. 51(4). p.201-205
abstract
Objective and design: To investigate the effect of enrofloxacin on endotoxin resorption during bovine Escherichia coli mastitis. Animals: 12 healthy early postpartum Holstein cows. Treatment: Mastitis was induced by intramammary infusion of 10(4) cfu E. coli P4:O32. Six cows were treated twice according to the usual enrofloxacin therapy: 5 mg/kg enrofloxacin 1) intravenously at 10 h and 2) subcutaneously at 30 h after challenge. The other 6 cows served as non-treated controls. Methods: Blood and milk samples were collected at several time points after challenge. LPS in plasma was quantified using the limulus amoebocyte lysate (LAL) assay. The somatic cell count (SCC) and cfu of milk samples were also analysed. Results: Occasional LPS peaks were detected in the plasma of 2 control cows at 6 h post-challenge and of 1 enrofloxacin-treated cow at 10 h post-challenge (P < 0.01 and P < 0.05, respectively, in comparison with time 0), just before enrofloxacin treatment. After enrofloxacin treatment, no significant LPS amounts were detected in the plasma of treated cows, but neither in the control cows. Conclusion: During induced coliform mastitis, LPS resorption in plasma occured only sporadically and within 10 h post-challenge. Whereas enrofloxacin treatment clearly limited bacterial growth in milk, significant effects on LPS resorption could not be detected. This suggests that enrofloxacin treatment of E. coli mastitis is predominantly beneficial by its bactericidal activity and is not associated with enhanced resorption of endotoxins.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
coliform mastitis, endotoxin resorption, enrofloxacin treatment, DAIRY-COWS, ANTIBIOTICS, NEUTROPHILS, CHEMOTAXIS, INFECTION, LEUKOCYTE, RELEASE, BURST, MILK
journal title
INFLAMMATION RESEARCH
Inflamm. Res.
volume
51
issue
4
pages
201-205 pages
Web of Science type
Article
Web of Science id
000175668700006
JCR category
IMMUNOLOGY
JCR impact factor
1.602 (2002)
JCR rank
76/119 (2002)
JCR quartile
3 (2002)
ISSN
1023-3830
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
149277
handle
http://hdl.handle.net/1854/LU-149277
alternative location
http://www.springerlink.com/content/yc13bdml12m6qdpp/fulltext.pdf
date created
2004-01-14 13:38:00
date last changed
2016-12-19 15:37:53
@article{149277,
  abstract     = {Objective and design: To investigate the effect of enrofloxacin on endotoxin resorption during bovine Escherichia coli mastitis.
Animals: 12 healthy early postpartum Holstein cows.
Treatment: Mastitis was induced by intramammary infusion of 10(4) cfu E. coli P4:O32. Six cows were treated twice according to the usual enrofloxacin therapy: 5 mg/kg enrofloxacin 1) intravenously at 10 h and 2) subcutaneously at 30 h after challenge. The other 6 cows served as non-treated controls.
Methods: Blood and milk samples were collected at several time points after challenge. LPS in plasma was quantified using the limulus amoebocyte lysate (LAL) assay. The somatic cell count (SCC) and cfu of milk samples were also analysed.
Results: Occasional LPS peaks were detected in the plasma of 2 control cows at 6 h post-challenge and of 1 enrofloxacin-treated cow at 10 h post-challenge (P {\textlangle} 0.01 and P {\textlangle} 0.05, respectively, in comparison with time 0), just before enrofloxacin treatment. After enrofloxacin treatment, no significant LPS amounts were detected in the plasma of treated cows, but neither in the control cows.
Conclusion: During induced coliform mastitis, LPS resorption in plasma occured only sporadically and within 10 h post-challenge. Whereas enrofloxacin treatment clearly limited bacterial growth in milk, significant effects on LPS resorption could not be detected. This suggests that enrofloxacin treatment of E. coli mastitis is predominantly beneficial by its bactericidal activity and is not associated with enhanced resorption of endotoxins.},
  author       = {Dosogne, Hilde and Meyer, Evelyne and STURK, A and VAN LOON, J and MASSART-LEEN, AM and Burvenich, Christian},
  issn         = {1023-3830},
  journal      = {INFLAMMATION RESEARCH},
  keyword      = {coliform mastitis,endotoxin resorption,enrofloxacin treatment,DAIRY-COWS,ANTIBIOTICS,NEUTROPHILS,CHEMOTAXIS,INFECTION,LEUKOCYTE,RELEASE,BURST,MILK},
  language     = {eng},
  number       = {4},
  pages        = {201--205},
  title        = {Effect of enrofloxacin treatment on plasma endotoxin during bovine Escherichia coli mastitis.},
  url          = {http://www.springerlink.com/content/yc13bdml12m6qdpp/fulltext.pdf},
  volume       = {51},
  year         = {2002},
}

Chicago
Dosogne, Hilde, Evelyne Meyer, A STURK, J VAN LOON, AM MASSART-LEEN, and Christian Burvenich. 2002. “Effect of Enrofloxacin Treatment on Plasma Endotoxin During Bovine Escherichia Coli Mastitis.” Inflammation Research 51 (4): 201–205.
APA
Dosogne, Hilde, Meyer, E., STURK, A., VAN LOON, J., MASSART-LEEN, A., & Burvenich, C. (2002). Effect of enrofloxacin treatment on plasma endotoxin during bovine Escherichia coli mastitis. INFLAMMATION RESEARCH, 51(4), 201–205.
Vancouver
1.
Dosogne H, Meyer E, STURK A, VAN LOON J, MASSART-LEEN A, Burvenich C. Effect of enrofloxacin treatment on plasma endotoxin during bovine Escherichia coli mastitis. INFLAMMATION RESEARCH. 2002;51(4):201–5.
MLA
Dosogne, Hilde, Evelyne Meyer, A STURK, et al. “Effect of Enrofloxacin Treatment on Plasma Endotoxin During Bovine Escherichia Coli Mastitis.” INFLAMMATION RESEARCH 51.4 (2002): 201–205. Print.