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Non destructive analysis of the wax layer of apple (Malus domestica Borkh.) by means of confocal laser scanning microscopy

Els A Veraverbeke, Nathalie Van Bruaene, Patric Van Oostveldt UGent and Bart M Nicolaï (2001) PLANTA. 213(4). p.525-533
abstract
Confocal laser scanning microscopy (CLSM) was used to non-destructively analyse the changes in the structure and thickness of the cuticle during storage of apples (Malus domestica Borkh.). Interpretation of the confocal images was performed by comparison with scanning electron microscopy and environmental scanning electron microscopy images. The natural reflectance of the wax and the auto-fluorescence of the underlying cells made it possible with CLSM to distinguish the wax from the underlying layers without any pretreatment of the fruit. The thickness of the consecutive layers (wax, cutin, cells) could be estimated from measurements of the reflection and fluorescence intensities as a function of the number of pixels. The mean wax-layer thickness measured in this way amounted to 2.58 mum, 3.41 mum or 4.14 mum for the cultivars Jonagold, Jonagored and Elstar, respectively. Changes in the wax structure and cells of the same important Belgian apple cultivars as mentioned above were monitored during nine months of storage in ultra low oxygen and after exposure to ambient conditions. The changes in the wax ultrastructure and cell morphology are likely related to water losses and specific protection of the apple cultivars against water losses during storage and shelf life.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
fruit storage, MUTANTS, cuticle, Malus (wax), wax (ultrastructure), GOLDEN DELICIOUS APPLES, EPICUTICULAR WAX, PLANT CUTICLES, EPIDERMAL CONDUCTANCE, SORGHUM-BICOLOR, CALCIUM-UPTAKE, WATER-VAPOR, STOMATA, FRUIT
journal title
PLANTA
Planta
volume
213
issue
4
pages
525 - 533
Web of Science type
Article
Web of Science id
000170680300004
JCR category
PLANT SCIENCES
JCR impact factor
3.349 (2001)
JCR rank
11/134 (2001)
JCR quartile
1 (2001)
ISSN
0032-0935
DOI
10.1007/s004250100528
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
148470
handle
http://hdl.handle.net/1854/LU-148470
date created
2004-01-14 13:38:00
date last changed
2016-12-19 15:38:20
@article{148470,
  abstract     = {Confocal laser scanning microscopy (CLSM) was used to non-destructively analyse the changes in the structure and thickness of the cuticle during storage of apples (Malus domestica Borkh.). Interpretation of the confocal images was performed by comparison with scanning electron microscopy and environmental scanning electron microscopy images. The natural reflectance of the wax and the auto-fluorescence of the underlying cells made it possible with CLSM to distinguish the wax from the underlying layers without any pretreatment of the fruit. The thickness of the consecutive layers (wax, cutin, cells) could be estimated from measurements of the reflection and fluorescence intensities as a function of the number of pixels. The mean wax-layer thickness measured in this way amounted to 2.58 mum, 3.41 mum or 4.14 mum for the cultivars Jonagold, Jonagored and Elstar, respectively. Changes in the wax structure and cells of the same important Belgian apple cultivars as mentioned above were monitored during nine months of storage in ultra low oxygen and after exposure to ambient conditions. The changes in the wax ultrastructure and cell morphology are likely related to water losses and specific protection of the apple cultivars against water losses during storage and shelf life.},
  author       = {Veraverbeke, Els A and Van Bruaene, Nathalie and Van Oostveldt, Patric and Nicola{\"i}, Bart M},
  issn         = {0032-0935},
  journal      = {PLANTA},
  keyword      = {fruit storage,MUTANTS,cuticle,Malus (wax),wax (ultrastructure),GOLDEN DELICIOUS APPLES,EPICUTICULAR WAX,PLANT CUTICLES,EPIDERMAL CONDUCTANCE,SORGHUM-BICOLOR,CALCIUM-UPTAKE,WATER-VAPOR,STOMATA,FRUIT},
  language     = {eng},
  number       = {4},
  pages        = {525--533},
  title        = {Non destructive analysis of the wax layer of apple (Malus domestica Borkh.) by means of confocal laser scanning microscopy},
  url          = {http://dx.doi.org/10.1007/s004250100528},
  volume       = {213},
  year         = {2001},
}

Chicago
Veraverbeke, Els A, Nathalie Van Bruaene, Patric Van Oostveldt, and Bart M Nicolaï. 2001. “Non Destructive Analysis of the Wax Layer of Apple (Malus Domestica Borkh.) by Means of Confocal Laser Scanning Microscopy.” Planta 213 (4): 525–533.
APA
Veraverbeke, E. A., Van Bruaene, N., Van Oostveldt, P., & Nicolaï, B. M. (2001). Non destructive analysis of the wax layer of apple (Malus domestica Borkh.) by means of confocal laser scanning microscopy. PLANTA, 213(4), 525–533.
Vancouver
1.
Veraverbeke EA, Van Bruaene N, Van Oostveldt P, Nicolaï BM. Non destructive analysis of the wax layer of apple (Malus domestica Borkh.) by means of confocal laser scanning microscopy. PLANTA. 2001;213(4):525–33.
MLA
Veraverbeke, Els A, Nathalie Van Bruaene, Patric Van Oostveldt, et al. “Non Destructive Analysis of the Wax Layer of Apple (Malus Domestica Borkh.) by Means of Confocal Laser Scanning Microscopy.” PLANTA 213.4 (2001): 525–533. Print.