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Preparation and sequencing of secreted proteins from the pharyngeal glands of the plant parasitic nematode Heterodera schachtii

Jan De Meutter (UGent) , Bartel Vanholme (UGent) , Guy Bauw (UGent) , Tom Tytgat (UGent) , Greetje Gheysen and Godelieve Gheysen (UGent)
(2001) MOLECULAR PLANT PATHOLOGY. 2(5). p.297-301
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Abstract
In order to gain insight into the biology of the parasitic relationship between Heterodera schachtii and its host plant, it is important to understand the functional role of the nematode's pharyngeal secretions. These secretions presumably play a key role in establishing and maintaining a feeding site for the nematode. An optimized method was used for the in vitro production of H. schachtii pharyngeal gland secretions. These pharyngeal secretions were mainly produced in an insoluble form and could be solubilized under denaturing conditions for further analysis. The soluble fraction was concentrated with StrataClean (Stratagene, La Jolla, CA) or with a phenol/ether extraction. These methods made it possible for the first time to separate the secreted proteins on two-dimensional gels. By combining a micropreparative sample preparation with mass spectrometry, two beta-1,4-endoglucanases were identified. A third spot was identified as a novel protein by microsequencing. This is the first report on protein sequence information from pharyngeal secretions of a plant parasitic nematode.

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Chicago
De Meutter, Jan, Bartel Vanholme, Guy Bauw, Tom Tytgat, Greetje Gheysen, and Godelieve Gheysen. 2001. “Preparation and Sequencing of Secreted Proteins from the Pharyngeal Glands of the Plant Parasitic Nematode Heterodera Schachtii.” Molecular Plant Pathology 2 (5): 297–301.
APA
De Meutter, J., Vanholme, B., Bauw, G., Tytgat, T., Gheysen, G., & Gheysen, G. (2001). Preparation and sequencing of secreted proteins from the pharyngeal glands of the plant parasitic nematode Heterodera schachtii. MOLECULAR PLANT PATHOLOGY, 2(5), 297–301.
Vancouver
1.
De Meutter J, Vanholme B, Bauw G, Tytgat T, Gheysen G, Gheysen G. Preparation and sequencing of secreted proteins from the pharyngeal glands of the plant parasitic nematode Heterodera schachtii. MOLECULAR PLANT PATHOLOGY. 2001;2(5):297–301.
MLA
De Meutter, Jan, Bartel Vanholme, Guy Bauw, et al. “Preparation and Sequencing of Secreted Proteins from the Pharyngeal Glands of the Plant Parasitic Nematode Heterodera Schachtii.” MOLECULAR PLANT PATHOLOGY 2.5 (2001): 297–301. Print.
@article{148433,
  abstract     = {In order to gain insight into the biology of the parasitic relationship between Heterodera schachtii and its host plant, it is important to understand the functional role of the nematode's pharyngeal secretions. These secretions presumably play a key role in establishing and maintaining a feeding site for the nematode. An optimized method was used for the in vitro production of H. schachtii pharyngeal gland secretions. These pharyngeal secretions were mainly produced in an insoluble form and could be solubilized under denaturing conditions for further analysis. The soluble fraction was concentrated with StrataClean (Stratagene, La Jolla, CA) or with a phenol/ether extraction. These methods made it possible for the first time to separate the secreted proteins on two-dimensional gels. By combining a micropreparative sample preparation with mass spectrometry, two beta-1,4-endoglucanases were identified. A third spot was identified as a novel protein by microsequencing. This is the first report on protein sequence information from pharyngeal secretions of a plant parasitic nematode.},
  author       = {De Meutter, Jan and Vanholme, Bartel and Bauw, Guy and Tytgat, Tom and Gheysen, Greetje and Gheysen, Godelieve},
  issn         = {1464-6722},
  journal      = {MOLECULAR PLANT PATHOLOGY},
  language     = {eng},
  number       = {5},
  pages        = {297--301},
  title        = {Preparation and sequencing of secreted proteins from the pharyngeal glands of the plant parasitic nematode Heterodera schachtii},
  url          = {http://dx.doi.org/10.1046/j.1464-6722.2001.00078.x},
  volume       = {2},
  year         = {2001},
}

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