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Preparation and sequencing of secreted proteins from the pharyngeal glands of the plant parasitic nematode Heterodera schachtii

Jan De Meutter UGent, Bartel Vanholme UGent, Guy Bauw UGent, Tom Tytgat UGent, Greetje Gheysen and Godelieve Gheysen UGent (2001) MOLECULAR PLANT PATHOLOGY. 2(5). p.297-301
abstract
In order to gain insight into the biology of the parasitic relationship between Heterodera schachtii and its host plant, it is important to understand the functional role of the nematode's pharyngeal secretions. These secretions presumably play a key role in establishing and maintaining a feeding site for the nematode. An optimized method was used for the in vitro production of H. schachtii pharyngeal gland secretions. These pharyngeal secretions were mainly produced in an insoluble form and could be solubilized under denaturing conditions for further analysis. The soluble fraction was concentrated with StrataClean (Stratagene, La Jolla, CA) or with a phenol/ether extraction. These methods made it possible for the first time to separate the secreted proteins on two-dimensional gels. By combining a micropreparative sample preparation with mass spectrometry, two beta-1,4-endoglucanases were identified. A third spot was identified as a novel protein by microsequencing. This is the first report on protein sequence information from pharyngeal secretions of a plant parasitic nematode.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
MOLECULAR PLANT PATHOLOGY
Mol. Plant Pathol.
volume
2
issue
5
pages
297 - 301
Web of Science type
Article
Web of Science id
000208116700006
ISSN
1464-6722
DOI
10.1046/j.1464-6722.2001.00078.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
148433
handle
http://hdl.handle.net/1854/LU-148433
date created
2004-01-14 13:38:00
date last changed
2016-12-19 15:38:32
@article{148433,
  abstract     = {In order to gain insight into the biology of the parasitic relationship between Heterodera schachtii and its host plant, it is important to understand the functional role of the nematode's pharyngeal secretions. These secretions presumably play a key role in establishing and maintaining a feeding site for the nematode. An optimized method was used for the in vitro production of H. schachtii pharyngeal gland secretions. These pharyngeal secretions were mainly produced in an insoluble form and could be solubilized under denaturing conditions for further analysis. The soluble fraction was concentrated with StrataClean (Stratagene, La Jolla, CA) or with a phenol/ether extraction. These methods made it possible for the first time to separate the secreted proteins on two-dimensional gels. By combining a micropreparative sample preparation with mass spectrometry, two beta-1,4-endoglucanases were identified. A third spot was identified as a novel protein by microsequencing. This is the first report on protein sequence information from pharyngeal secretions of a plant parasitic nematode.},
  author       = {De Meutter, Jan and Vanholme, Bartel and Bauw, Guy and Tytgat, Tom and Gheysen, Greetje and Gheysen, Godelieve},
  issn         = {1464-6722},
  journal      = {MOLECULAR PLANT PATHOLOGY},
  language     = {eng},
  number       = {5},
  pages        = {297--301},
  title        = {Preparation and sequencing of secreted proteins from the pharyngeal glands of the plant parasitic nematode Heterodera schachtii},
  url          = {http://dx.doi.org/10.1046/j.1464-6722.2001.00078.x},
  volume       = {2},
  year         = {2001},
}

Chicago
De Meutter, Jan, Bartel Vanholme, Guy Bauw, Tom Tytgat, Greetje Gheysen, and Godelieve Gheysen. 2001. “Preparation and Sequencing of Secreted Proteins from the Pharyngeal Glands of the Plant Parasitic Nematode Heterodera Schachtii.” Molecular Plant Pathology 2 (5): 297–301.
APA
De Meutter, J., Vanholme, B., Bauw, G., Tytgat, T., Gheysen, G., & Gheysen, G. (2001). Preparation and sequencing of secreted proteins from the pharyngeal glands of the plant parasitic nematode Heterodera schachtii. MOLECULAR PLANT PATHOLOGY, 2(5), 297–301.
Vancouver
1.
De Meutter J, Vanholme B, Bauw G, Tytgat T, Gheysen G, Gheysen G. Preparation and sequencing of secreted proteins from the pharyngeal glands of the plant parasitic nematode Heterodera schachtii. MOLECULAR PLANT PATHOLOGY. 2001;2(5):297–301.
MLA
De Meutter, Jan, Bartel Vanholme, Guy Bauw, et al. “Preparation and Sequencing of Secreted Proteins from the Pharyngeal Glands of the Plant Parasitic Nematode Heterodera Schachtii.” MOLECULAR PLANT PATHOLOGY 2.5 (2001): 297–301. Print.