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Development of a rapid pH-based biosensor to monitor and control the hygienic quality of reclaimed domestic wastewater

Tom Dewettinck, Kris Van Hege and Willy Verstraete (2001) APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 56(5-6). p.809-815
abstract
The re-use of treated domestic wastewater necessitates a rigorous control and rapid monitoring of the hygienic quality of the reclaimed water. For this purpose, a new pH-based biosensor was developed. The essence of the methodology of the sensor is the monitoring of the acidification due to bacterial metabolism of added glucose. To improve the sensitivity, the alkalinity of the water sample is reduced prior to monitoring the acidification. This is done by stripping CO2 at a neutral or acidic pH value. The hygienic aspect of the sensor lies in the applied temperature (37 degreesC) and the use of N-2 as decarbonizing gas, thus creating conditions favorable for enteric bacteria. The developed sensor could be used onsite at an advanced stage of treatment, as an endpoint or intake quality control device. For both applications. a useful correlation was obtained between log total plate count and lag time or acidification rate, respectively. Absolute detection limits lay in the ranges of either 10(3) colony-forming units (CFU)/ml in 6 h (endpoint quality control), or less than 10(5) CFU/ml in 1 h (intake quality control).
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
BACTERIA
journal title
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Appl. Microbiol. Biotechnol.
volume
56
issue
5-6
pages
809-815 pages
Web of Science type
Article
Web of Science id
000171273500032
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
1.754 (2001)
JCR rank
42/130 (2001)
JCR quartile
2 (2001)
ISSN
0175-7598
DOI
10.1007/s002530100705
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
147423
handle
http://hdl.handle.net/1854/LU-147423
date created
2004-01-14 13:38:00
date last changed
2016-12-19 15:38:32
@article{147423,
  abstract     = {The re-use of treated domestic wastewater necessitates a rigorous control and rapid monitoring of the hygienic quality of the reclaimed water. For this purpose, a new pH-based biosensor was developed. The essence of the methodology of the sensor is the monitoring of the acidification due to bacterial metabolism of added glucose. To improve the sensitivity, the alkalinity of the water sample is reduced prior to monitoring the acidification. This is done by stripping CO2 at a neutral or acidic pH value. The hygienic aspect of the sensor lies in the applied temperature (37 degreesC) and the use of N-2 as decarbonizing gas, thus creating conditions favorable for enteric bacteria. The developed sensor could be used onsite at an advanced stage of treatment, as an endpoint or intake quality control device. For both applications. a useful correlation was obtained between log total plate count and lag time or acidification rate, respectively. Absolute detection limits lay in the ranges of either 10(3) colony-forming units (CFU)/ml in 6 h (endpoint quality control), or less than 10(5) CFU/ml in 1 h (intake quality control).},
  author       = {Dewettinck, Tom and Van Hege, Kris and Verstraete, Willy},
  issn         = {0175-7598},
  journal      = {APPLIED MICROBIOLOGY AND BIOTECHNOLOGY},
  keyword      = {BACTERIA},
  language     = {eng},
  number       = {5-6},
  pages        = {809--815},
  title        = {Development of a rapid pH-based biosensor to monitor and control the hygienic quality of reclaimed domestic wastewater},
  url          = {http://dx.doi.org/10.1007/s002530100705},
  volume       = {56},
  year         = {2001},
}

Chicago
Dewettinck, Tom, Kris Van Hege, and Willy Verstraete. 2001. “Development of a Rapid pH-based Biosensor to Monitor and Control the Hygienic Quality of Reclaimed Domestic Wastewater.” Applied Microbiology and Biotechnology 56 (5-6): 809–815.
APA
Dewettinck, T., Van Hege, K., & Verstraete, W. (2001). Development of a rapid pH-based biosensor to monitor and control the hygienic quality of reclaimed domestic wastewater. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 56(5-6), 809–815.
Vancouver
1.
Dewettinck T, Van Hege K, Verstraete W. Development of a rapid pH-based biosensor to monitor and control the hygienic quality of reclaimed domestic wastewater. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2001;56(5-6):809–15.
MLA
Dewettinck, Tom, Kris Van Hege, and Willy Verstraete. “Development of a Rapid pH-based Biosensor to Monitor and Control the Hygienic Quality of Reclaimed Domestic Wastewater.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 56.5-6 (2001): 809–815. Print.