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Induction of homozygous Spathiphyllum wallisii genotypes through gynogenesis

Tom Eeckhaut, Stefaan Werbrouck UGent, Jochen Dendauw, Erik Van Bockstaele and Pierre Debergh (2001) PLANT CELL TISSUE AND ORGAN CULTURE. 67(2). p.181-189
abstract
In Spathiphyllum wallisii the production of doubled haploids was attempted. Different combinations of growth regulators were tested, as well as different cultivars. The use of TDZ (0.25-1 muM) in ovary cultures of Spathiphyllum was required. On the contrary, cytokinins were not crucial during ovule culture; in fact, the use of a too high TDZ concentration induced diploid parthenogenesis in ovules of `Alfa'. The use of the imidazole fungicides IMA, PRO or TRI during ovary culture was not critical, though they enhanced the swelling of ovules during ovary culture, making the isolation of the ovules easier. The ovule cultures yielded different plantlets. Flow cytometry and AFLP-patterns showed that two doubled haploid genotypes could be obtained from `Stefanie'. These plants showed a normal phenotype. We concluded that the induction of homozygous Spathiphyllum through gynogenesis is possible and is genotype dependent.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
in vitro, homozygous, ovule culture, Spathiphyllum, AFLP MARKERS, IMIDAZOLE FUNGICIDES, GENETIC DIVERSITY, IN-VITRO, CULTURE, INVITRO, ARACEAE, TOOL, AFLP, gynogenesis
journal title
PLANT CELL TISSUE AND ORGAN CULTURE
Plant Cell Tissue Organ Cult.
volume
67
issue
2
pages
181 - 189
Web of Science type
Article
Web of Science id
000171299800009
JCR category
PLANT SCIENCES
JCR impact factor
0.631 (2001)
JCR rank
80/134 (2001)
JCR quartile
3 (2001)
ISSN
0167-6857
DOI
10.1023/A:1011902108132
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
147136
handle
http://hdl.handle.net/1854/LU-147136
date created
2004-01-14 13:38:00
date last changed
2016-12-19 15:38:17
@article{147136,
  abstract     = {In Spathiphyllum wallisii the production of doubled haploids was attempted. Different combinations of growth regulators were tested, as well as different cultivars. The use of TDZ (0.25-1 muM) in ovary cultures of Spathiphyllum was required. On the contrary, cytokinins were not crucial during ovule culture; in fact, the use of a too high TDZ concentration induced diploid parthenogenesis in ovules of `Alfa'. The use of the imidazole fungicides IMA, PRO or TRI during ovary culture was not critical, though they enhanced the swelling of ovules during ovary culture, making the isolation of the ovules easier. The ovule cultures yielded different plantlets. Flow cytometry and AFLP-patterns showed that two doubled haploid genotypes could be obtained from `Stefanie'. These plants showed a normal phenotype. We concluded that the induction of homozygous Spathiphyllum through gynogenesis is possible and is genotype dependent.},
  author       = {Eeckhaut, Tom and Werbrouck, Stefaan and Dendauw, Jochen and Van Bockstaele, Erik and Debergh, Pierre},
  issn         = {0167-6857},
  journal      = {PLANT CELL TISSUE AND ORGAN CULTURE},
  keyword      = {in vitro,homozygous,ovule culture,Spathiphyllum,AFLP MARKERS,IMIDAZOLE FUNGICIDES,GENETIC DIVERSITY,IN-VITRO,CULTURE,INVITRO,ARACEAE,TOOL,AFLP,gynogenesis},
  language     = {eng},
  number       = {2},
  pages        = {181--189},
  title        = {Induction of homozygous Spathiphyllum wallisii genotypes through gynogenesis},
  url          = {http://dx.doi.org/10.1023/A:1011902108132},
  volume       = {67},
  year         = {2001},
}

Chicago
Eeckhaut, Tom, Stefaan Werbrouck, Jochen Dendauw, Erik Van Bockstaele, and Pierre Debergh. 2001. “Induction of Homozygous Spathiphyllum Wallisii Genotypes Through Gynogenesis.” Plant Cell Tissue and Organ Culture 67 (2): 181–189.
APA
Eeckhaut, T., Werbrouck, S., Dendauw, J., Van Bockstaele, E., & Debergh, P. (2001). Induction of homozygous Spathiphyllum wallisii genotypes through gynogenesis. PLANT CELL TISSUE AND ORGAN CULTURE, 67(2), 181–189.
Vancouver
1.
Eeckhaut T, Werbrouck S, Dendauw J, Van Bockstaele E, Debergh P. Induction of homozygous Spathiphyllum wallisii genotypes through gynogenesis. PLANT CELL TISSUE AND ORGAN CULTURE. 2001;67(2):181–9.
MLA
Eeckhaut, Tom, Stefaan Werbrouck, Jochen Dendauw, et al. “Induction of Homozygous Spathiphyllum Wallisii Genotypes Through Gynogenesis.” PLANT CELL TISSUE AND ORGAN CULTURE 67.2 (2001): 181–189. Print.