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Induction of homozygous Spathiphyllum wallisii genotypes through gynogenesis

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Abstract
In Spathiphyllum wallisii the production of doubled haploids was attempted. Different combinations of growth regulators were tested, as well as different cultivars. The use of TDZ (0.25-1 muM) in ovary cultures of Spathiphyllum was required. On the contrary, cytokinins were not crucial during ovule culture; in fact, the use of a too high TDZ concentration induced diploid parthenogenesis in ovules of `Alfa'. The use of the imidazole fungicides IMA, PRO or TRI during ovary culture was not critical, though they enhanced the swelling of ovules during ovary culture, making the isolation of the ovules easier. The ovule cultures yielded different plantlets. Flow cytometry and AFLP-patterns showed that two doubled haploid genotypes could be obtained from `Stefanie'. These plants showed a normal phenotype. We concluded that the induction of homozygous Spathiphyllum through gynogenesis is possible and is genotype dependent.
Keywords
in vitro, homozygous, ovule culture, Spathiphyllum, AFLP MARKERS, IMIDAZOLE FUNGICIDES, GENETIC DIVERSITY, IN-VITRO, CULTURE, INVITRO, ARACEAE, TOOL, AFLP, gynogenesis

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Citation

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Chicago
Eeckhaut, Tom, Stefaan Werbrouck, Jochen Dendauw, Erik Van Bockstaele, and Pierre Debergh. 2001. “Induction of Homozygous Spathiphyllum Wallisii Genotypes Through Gynogenesis.” Plant Cell Tissue and Organ Culture 67 (2): 181–189.
APA
Eeckhaut, T., Werbrouck, S., Dendauw, J., Van Bockstaele, E., & Debergh, P. (2001). Induction of homozygous Spathiphyllum wallisii genotypes through gynogenesis. PLANT CELL TISSUE AND ORGAN CULTURE, 67(2), 181–189.
Vancouver
1.
Eeckhaut T, Werbrouck S, Dendauw J, Van Bockstaele E, Debergh P. Induction of homozygous Spathiphyllum wallisii genotypes through gynogenesis. PLANT CELL TISSUE AND ORGAN CULTURE. 2001;67(2):181–9.
MLA
Eeckhaut, Tom, Stefaan Werbrouck, Jochen Dendauw, et al. “Induction of Homozygous Spathiphyllum Wallisii Genotypes Through Gynogenesis.” PLANT CELL TISSUE AND ORGAN CULTURE 67.2 (2001): 181–189. Print.
@article{147136,
  abstract     = {In Spathiphyllum wallisii the production of doubled haploids was attempted. Different combinations of growth regulators were tested, as well as different cultivars. The use of TDZ (0.25-1 muM) in ovary cultures of Spathiphyllum was required. On the contrary, cytokinins were not crucial during ovule culture; in fact, the use of a too high TDZ concentration induced diploid parthenogenesis in ovules of `Alfa'. The use of the imidazole fungicides IMA, PRO or TRI during ovary culture was not critical, though they enhanced the swelling of ovules during ovary culture, making the isolation of the ovules easier. The ovule cultures yielded different plantlets. Flow cytometry and AFLP-patterns showed that two doubled haploid genotypes could be obtained from `Stefanie'. These plants showed a normal phenotype. We concluded that the induction of homozygous Spathiphyllum through gynogenesis is possible and is genotype dependent.},
  author       = {Eeckhaut, Tom and Werbrouck, Stefaan and Dendauw, Jochen and Van Bockstaele, Erik and Debergh, Pierre},
  issn         = {0167-6857},
  journal      = {PLANT CELL TISSUE AND ORGAN CULTURE},
  keyword      = {in vitro,homozygous,ovule culture,Spathiphyllum,AFLP MARKERS,IMIDAZOLE FUNGICIDES,GENETIC DIVERSITY,IN-VITRO,CULTURE,INVITRO,ARACEAE,TOOL,AFLP,gynogenesis},
  language     = {eng},
  number       = {2},
  pages        = {181--189},
  title        = {Induction of homozygous Spathiphyllum wallisii genotypes through gynogenesis},
  url          = {http://dx.doi.org/10.1023/A:1011902108132},
  volume       = {67},
  year         = {2001},
}

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