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Culture of bovine bone marrow progenitor cells in vitro.

Valérie Van Merris, M Lenjou, Dagmar Hoeben, G Nijs, D Van Bockstaele and Christian Burvenich UGent (2001) VETERINARY QUARTERLY. 23(4). p.170-175
abstract
In vitro methylcellulose cultures of bovine bone marrow progenitor cells were developed. An existing technique described for bovine species was compared to a method for human tissue and further adapted during subsequent experiments. Bovine bone marrow samples were collected at the slaughterhouse, and mononuclear cells were separated by gradient centrifugation (1.077 g/ml specific density and 400g). The use of 3% bovine leucocyte-conditioned medium, produced by stimulation of blood lymphocytes with 4 mug/ml concanavalin A and harvested on day 4 of culture, gave better results than the use of supernatant of the human bladder carcinoma 5637, which is widely used in human bone marrow cultures. However, bovine leucocyte-conditioned medium was not added to erythroid cultures because inhibitory effects were observed. Erythroid colonies were stimulated with erythropoietin, and hemin was added to enable microscopic identification. Reduced oxygen tension was necessary to induce growth of erythroid colonies. This was not necessary for myeloid cultures. In conclusion, the results of this study show that the growth of myeloid and erythroid colonies in methylcellulose-based medium requires different culture conditions, which are different from the culture conditions for human cells.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SEPARATION, LEUKOCYTE, CATTLE, DAIRY-COWS, NEUTROPHIL FUNCTION, TRYPANOSOMA-CONGOLENSE, MESSENGER-RNA EXPRESSION, MACROPHAGE COLONY FORMATION, PERIOD, bone marrow, cattle, cell culture, ESCHERICHIA-COLI MASTITIS
journal title
VETERINARY QUARTERLY
Vet. Q.
volume
23
issue
4
pages
170-175 pages
Web of Science type
Article
Web of Science id
000173087000006
JCR category
VETERINARY SCIENCES
JCR impact factor
0.808 (2001)
JCR rank
38/126 (2001)
JCR quartile
2 (2001)
ISSN
0165-2176
language
English
UGent publication?
yes
classification
A1
additional info
FT N/A
id
145763
handle
http://hdl.handle.net/1854/LU-145763
date created
2004-01-14 13:38:00
date last changed
2016-12-19 15:38:34
@article{145763,
  abstract     = {In vitro methylcellulose cultures of bovine bone marrow progenitor cells were developed. An existing technique described for bovine species was compared to a method for human tissue and further adapted during subsequent experiments. Bovine bone marrow samples were collected at the slaughterhouse, and mononuclear cells were separated by gradient centrifugation (1.077 g/ml specific density and 400g). The use of 3\% bovine leucocyte-conditioned medium, produced by stimulation of blood lymphocytes with 4 mug/ml concanavalin A and harvested on day 4 of culture, gave better results than the use of supernatant of the human bladder carcinoma 5637, which is widely used in human bone marrow cultures. However, bovine leucocyte-conditioned medium was not added to erythroid cultures because inhibitory effects were observed. Erythroid colonies were stimulated with erythropoietin, and hemin was added to enable microscopic identification. Reduced oxygen tension was necessary to induce growth of erythroid colonies. This was not necessary for myeloid cultures. In conclusion, the results of this study show that the growth of myeloid and erythroid colonies in methylcellulose-based medium requires different culture conditions, which are different from the culture conditions for human cells.},
  author       = {Van Merris, Val{\'e}rie and Lenjou, M and Hoeben, Dagmar and Nijs, G and Van Bockstaele, D and Burvenich, Christian},
  issn         = {0165-2176},
  journal      = {VETERINARY QUARTERLY},
  keyword      = {SEPARATION,LEUKOCYTE,CATTLE,DAIRY-COWS,NEUTROPHIL FUNCTION,TRYPANOSOMA-CONGOLENSE,MESSENGER-RNA EXPRESSION,MACROPHAGE COLONY FORMATION,PERIOD,bone marrow,cattle,cell culture,ESCHERICHIA-COLI MASTITIS},
  language     = {eng},
  number       = {4},
  pages        = {170--175},
  title        = {Culture of bovine bone marrow progenitor cells in vitro.},
  volume       = {23},
  year         = {2001},
}

Chicago
Van Merris, Valérie, M Lenjou, Dagmar Hoeben, G Nijs, D Van Bockstaele, and Christian Burvenich. 2001. “Culture of Bovine Bone Marrow Progenitor Cells in Vitro.” Veterinary Quarterly 23 (4): 170–175.
APA
Van Merris, V., Lenjou, M., Hoeben, D., Nijs, G., Van Bockstaele, D., & Burvenich, C. (2001). Culture of bovine bone marrow progenitor cells in vitro. VETERINARY QUARTERLY, 23(4), 170–175.
Vancouver
1.
Van Merris V, Lenjou M, Hoeben D, Nijs G, Van Bockstaele D, Burvenich C. Culture of bovine bone marrow progenitor cells in vitro. VETERINARY QUARTERLY. 2001;23(4):170–5.
MLA
Van Merris, Valérie, M Lenjou, Dagmar Hoeben, et al. “Culture of Bovine Bone Marrow Progenitor Cells in Vitro.” VETERINARY QUARTERLY 23.4 (2001): 170–175. Print.