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Culture of bovine bone marrow progenitor cells in vitro.

(2001) VETERINARY QUARTERLY. 23(4). p.170-175
Author
Organization
Abstract
In vitro methylcellulose cultures of bovine bone marrow progenitor cells were developed. An existing technique described for bovine species was compared to a method for human tissue and further adapted during subsequent experiments. Bovine bone marrow samples were collected at the slaughterhouse, and mononuclear cells were separated by gradient centrifugation (1.077 g/ml specific density and 400g). The use of 3% bovine leucocyte-conditioned medium, produced by stimulation of blood lymphocytes with 4 mug/ml concanavalin A and harvested on day 4 of culture, gave better results than the use of supernatant of the human bladder carcinoma 5637, which is widely used in human bone marrow cultures. However, bovine leucocyte-conditioned medium was not added to erythroid cultures because inhibitory effects were observed. Erythroid colonies were stimulated with erythropoietin, and hemin was added to enable microscopic identification. Reduced oxygen tension was necessary to induce growth of erythroid colonies. This was not necessary for myeloid cultures. In conclusion, the results of this study show that the growth of myeloid and erythroid colonies in methylcellulose-based medium requires different culture conditions, which are different from the culture conditions for human cells.
Keywords
SEPARATION, LEUKOCYTE, CATTLE, DAIRY-COWS, NEUTROPHIL FUNCTION, TRYPANOSOMA-CONGOLENSE, MESSENGER-RNA EXPRESSION, MACROPHAGE COLONY FORMATION, PERIOD, bone marrow, cattle, cell culture, ESCHERICHIA-COLI MASTITIS

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Chicago
Van Merris, Valérie, M Lenjou, Dagmar Hoeben, G Nijs, D Van Bockstaele, and Christian Burvenich. 2001. “Culture of Bovine Bone Marrow Progenitor Cells in Vitro.” Veterinary Quarterly 23 (4): 170–175.
APA
Van Merris, V., Lenjou, M., Hoeben, D., Nijs, G., Van Bockstaele, D., & Burvenich, C. (2001). Culture of bovine bone marrow progenitor cells in vitro. VETERINARY QUARTERLY, 23(4), 170–175.
Vancouver
1.
Van Merris V, Lenjou M, Hoeben D, Nijs G, Van Bockstaele D, Burvenich C. Culture of bovine bone marrow progenitor cells in vitro. VETERINARY QUARTERLY. 2001;23(4):170–5.
MLA
Van Merris, Valérie, M Lenjou, Dagmar Hoeben, et al. “Culture of Bovine Bone Marrow Progenitor Cells in Vitro.” VETERINARY QUARTERLY 23.4 (2001): 170–175. Print.
@article{145763,
  abstract     = {In vitro methylcellulose cultures of bovine bone marrow progenitor cells were developed. An existing technique described for bovine species was compared to a method for human tissue and further adapted during subsequent experiments. Bovine bone marrow samples were collected at the slaughterhouse, and mononuclear cells were separated by gradient centrifugation (1.077 g/ml specific density and 400g). The use of 3\% bovine leucocyte-conditioned medium, produced by stimulation of blood lymphocytes with 4 mug/ml concanavalin A and harvested on day 4 of culture, gave better results than the use of supernatant of the human bladder carcinoma 5637, which is widely used in human bone marrow cultures. However, bovine leucocyte-conditioned medium was not added to erythroid cultures because inhibitory effects were observed. Erythroid colonies were stimulated with erythropoietin, and hemin was added to enable microscopic identification. Reduced oxygen tension was necessary to induce growth of erythroid colonies. This was not necessary for myeloid cultures. In conclusion, the results of this study show that the growth of myeloid and erythroid colonies in methylcellulose-based medium requires different culture conditions, which are different from the culture conditions for human cells.},
  author       = {Van Merris, Val{\'e}rie and Lenjou, M and Hoeben, Dagmar and Nijs, G and Van Bockstaele, D and Burvenich, Christian},
  issn         = {0165-2176},
  journal      = {VETERINARY QUARTERLY},
  keyword      = {SEPARATION,LEUKOCYTE,CATTLE,DAIRY-COWS,NEUTROPHIL FUNCTION,TRYPANOSOMA-CONGOLENSE,MESSENGER-RNA EXPRESSION,MACROPHAGE COLONY FORMATION,PERIOD,bone marrow,cattle,cell culture,ESCHERICHIA-COLI MASTITIS},
  language     = {eng},
  number       = {4},
  pages        = {170--175},
  title        = {Culture of bovine bone marrow progenitor cells in vitro.},
  volume       = {23},
  year         = {2001},
}

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