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Carbohydrate-deficient transferrin and chronic alcohol ingestion in subjects with transferrin CD-variants

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Abstract
Carbohydrate-deficient transferrin (CDT) is widely accepted as screening test for excessive alcohol consumption. However, results from subjects with transferrin variants must be interpreted with caution since chromatography-based methods may give false-positive results. Furthermore, due to the co-elution in HPLC or the co-migration in capillary zone elctrophoresis (CZE) of the di- and trisialylated C transferrins with the tetrasialylated D peak, exact measurement of CDT is impossible in CD-variants. Therefore, in this study, we tried to offer a different solution, including only the asialo-D, asialo-C, monosialo-D, monosialo-C, disialoD and trisialo-D transferrins in the CDT calculation and referring to a different cut-off value for CDT in transferrin CD-variants. Comparison of alcohol consumers with teetotalers demonstrated area under the receiver operating characteristic curve of 0.79 and 0.76 for carbohydrate-def icient transferrin, 0.71 and 0.71 for mean corpuscular volume and 0.51 and 0.68 for gamma -glutamyl-transferase in 43 subjects with transferrin CD-variants and 225 subjects with CC-phenotypes, respectively. Since false-positive carbohydrate-deficient transferrin results due to a transferrin CD-variant have major social implications, capillary electrophoresis-based or similar methods (HPLC, FPLC) should be preferred in populations carrying a high D-allele frequency.
Keywords
capillary electrophoresis, alcoholism, carbohydrate-deficient transferrin, transferrin, GAMMA-GLUTAMYL-TRANSFERASE, MEAN CORPUSCULAR VOLUME, SERUM TRANSFERRIN, LABORATORY TESTS, CONSUMPTION, SENSITIVITY, ISOFORMS, ASSAY, ABUSE

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Chicago
Wuyts, Brigitte, Joris Delanghe, Ishmael Kasvosve, Victor R Gordeuk, Innocent T Gangaidzo, and Zvenyika AR Gomo. 2001. “Carbohydrate-deficient Transferrin and Chronic Alcohol Ingestion in Subjects with Transferrin CD-variants.” Clinical Chemistry and Laboratory Medicine 39 (10): 937–943.
APA
Wuyts, Brigitte, Delanghe, J., Kasvosve, I., Gordeuk, V. R., Gangaidzo, I. T., & Gomo, Z. A. (2001). Carbohydrate-deficient transferrin and chronic alcohol ingestion in subjects with transferrin CD-variants. CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 39(10), 937–943.
Vancouver
1.
Wuyts B, Delanghe J, Kasvosve I, Gordeuk VR, Gangaidzo IT, Gomo ZA. Carbohydrate-deficient transferrin and chronic alcohol ingestion in subjects with transferrin CD-variants. CLINICAL CHEMISTRY AND LABORATORY MEDICINE. 2001;39(10):937–43.
MLA
Wuyts, Brigitte, Joris Delanghe, Ishmael Kasvosve, et al. “Carbohydrate-deficient Transferrin and Chronic Alcohol Ingestion in Subjects with Transferrin CD-variants.” CLINICAL CHEMISTRY AND LABORATORY MEDICINE 39.10 (2001): 937–943. Print.
@article{141758,
  abstract     = {Carbohydrate-deficient transferrin (CDT) is widely accepted as screening test for excessive alcohol consumption. However, results from subjects with transferrin variants must be interpreted with caution since chromatography-based methods may give false-positive results. Furthermore, due to the co-elution in HPLC or the co-migration in capillary zone elctrophoresis (CZE) of the di- and trisialylated C transferrins with the tetrasialylated D peak, exact measurement of CDT is impossible in CD-variants. Therefore, in this study, we tried to offer a different solution, including only the asialo-D, asialo-C, monosialo-D, monosialo-C, disialoD and trisialo-D transferrins in the CDT calculation and referring to a different cut-off value for CDT in transferrin CD-variants. Comparison of alcohol consumers with teetotalers demonstrated area under the receiver operating characteristic curve of 0.79 and 0.76 for carbohydrate-def icient transferrin, 0.71 and 0.71 for mean corpuscular volume and 0.51 and 0.68 for gamma -glutamyl-transferase in 43 subjects with transferrin CD-variants and 225 subjects with CC-phenotypes, respectively. Since false-positive carbohydrate-deficient transferrin results due to a transferrin CD-variant have major social implications, capillary electrophoresis-based or similar methods (HPLC, FPLC) should be preferred in populations carrying a high D-allele frequency.},
  author       = {Wuyts, Brigitte and Delanghe, Joris and Kasvosve, Ishmael and Gordeuk, Victor R and Gangaidzo, Innocent T and Gomo, Zvenyika AR},
  issn         = {1434-6621},
  journal      = {CLINICAL CHEMISTRY AND LABORATORY MEDICINE},
  keyword      = {capillary electrophoresis,alcoholism,carbohydrate-deficient transferrin,transferrin,GAMMA-GLUTAMYL-TRANSFERASE,MEAN CORPUSCULAR VOLUME,SERUM TRANSFERRIN,LABORATORY TESTS,CONSUMPTION,SENSITIVITY,ISOFORMS,ASSAY,ABUSE},
  language     = {eng},
  number       = {10},
  pages        = {937--943},
  title        = {Carbohydrate-deficient transferrin and chronic alcohol ingestion in subjects with transferrin CD-variants},
  url          = {http://dx.doi.org/10.1515/CCLM.2001.150},
  volume       = {39},
  year         = {2001},
}

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