Ghent University Academic Bibliography

Advanced

Comparison of the antimicrobial tolerance of oxytetracycline-resistant heterotrophic bacteria isolated from hospital sewage and freshwater fishfarm water in Belgium

Geert Huys UGent, Dirk Gevers UGent, Robin Temmerman, Margo Cnockaert UGent, Rik Denys, Glenn Rhodes, Roger Pickup, Patrick McGann, Maura Hiney and Peter Smith, et al. (2001) SYSTEMATIC AND APPLIED MICROBIOLOGY. 24(1). p.122-130
abstract
The aim of this study was to investigate the relationship between antimicrobial tolerance and taxonomic diversity among the culturable oxyretracycline-resistant (Ot(r)) heterotrophic bacterial population in two Belgian aquatic sites receiving wastewater either from human medicine or from aquaculture. The study of Ot(r) heterotrophs and mesophilic Aeromonas spp. allowed comparison of tolerance data at the inter-genus as well as at the intragenus level. In total, 354 independently obtained Ot(r) isolates were subjected to antimicrobial tolerance testing and identified by GLC analysis of their cellular fatty acid methyl esters (FAMEs), by API 20E profiling and/or by Fluorescent Amplified Fragment Length Polymorphism (FAFLP) DNA fingerprinting. In general, Ot(r) hospital heterotrophs displayed a higher frequency (84%) of ampicillin (Amp) tolerance compared to the Ot(r) heterotrophs from the freshwater fishfarm site (22%). FAME results indicated that this effect was linked to the predominance of intrinsically ampicillin-resistant Ot(r) Aeromonas strains over representatives of Acinetobacter and Escherichia coli within the hospital strain set. Among the Ot(r) mesophilic Aeromonas strain set, the global tolerance profiles of the two sites only differed in a higher number of kanamycin (Kan) -tolerant strains (43%) for hospital aeromonads in comparison with the fishfarm aeromonads (8%). To some extent, this finding was correlated with the specific presence of Aeromonas caviae DNA hybridisation group (HG) 4. Collectively, these results suggest that the profiles for Amp and Kan tolerance observed in both sites arose from taxonomic differences in the culturable Ot(r) bacterial population at the generic or subgeneric level. In addition, our identification data also revealed that Enterobacter sp., Stenotrophomonas maltophilia, and A. veronii biovar sobria HG8 may be considered potential indicator organisms to assess microbial tolerance in various compartments of the aquatic environment.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
hospital sewage, oxytetracycline resistance, freshwater fishfarm, ANTIBIOTIC-RESISTANCE, PRODUCTION PLANTS, AEROMONAS, DIVERSITY, EFFLUENT, SAMPLES, RIVERS
journal title
SYSTEMATIC AND APPLIED MICROBIOLOGY
Syst. Appl. Microbiol.
volume
24
issue
1
pages
122 - 130
Web of Science type
Article
Web of Science id
000168850600015
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
2.054 (2001)
JCR rank
33/130 (2001)
JCR quartile
1 (2001)
ISSN
0723-2020
DOI
10.1078/0723-2020-00008
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
139038
handle
http://hdl.handle.net/1854/LU-139038
date created
2004-01-14 13:37:00
date last changed
2013-12-16 16:42:48
@article{139038,
  abstract     = {The aim of this study was to investigate the relationship between antimicrobial tolerance and taxonomic diversity among the culturable oxyretracycline-resistant (Ot(r)) heterotrophic bacterial population in two Belgian aquatic sites receiving wastewater either from human medicine or from aquaculture. The study of Ot(r) heterotrophs and mesophilic Aeromonas spp. allowed comparison of tolerance data at the inter-genus as well as at the intragenus level. In total, 354 independently obtained Ot(r) isolates were subjected to antimicrobial tolerance testing and identified by GLC analysis of their cellular fatty acid methyl esters (FAMEs), by API 20E profiling and/or by Fluorescent Amplified Fragment Length Polymorphism (FAFLP) DNA fingerprinting. In general, Ot(r) hospital heterotrophs displayed a higher frequency (84\%) of ampicillin (Amp) tolerance compared to the Ot(r) heterotrophs from the freshwater fishfarm site (22\%). FAME results indicated that this effect was linked to the predominance of intrinsically ampicillin-resistant Ot(r) Aeromonas strains over representatives of Acinetobacter and Escherichia coli within the hospital strain set. Among the Ot(r) mesophilic Aeromonas strain set, the global tolerance profiles of the two sites only differed in a higher number of kanamycin (Kan) -tolerant strains (43\%) for hospital aeromonads in comparison with the fishfarm aeromonads (8\%). To some extent, this finding was correlated with the specific presence of Aeromonas caviae DNA hybridisation group (HG) 4. Collectively, these results suggest that the profiles for Amp and Kan tolerance observed in both sites arose from taxonomic differences in the culturable Ot(r) bacterial population at the generic or subgeneric level. In addition, our identification data also revealed that Enterobacter sp., Stenotrophomonas maltophilia, and A. veronii biovar sobria HG8 may be considered potential indicator organisms to assess microbial tolerance in various compartments of the aquatic environment.},
  author       = {Huys, Geert and Gevers, Dirk and Temmerman, Robin and Cnockaert, Margo and Denys, Rik and Rhodes, Glenn and Pickup, Roger and McGann, Patrick and Hiney, Maura and Smith, Peter and Swings, Jean},
  issn         = {0723-2020},
  journal      = {SYSTEMATIC AND APPLIED MICROBIOLOGY},
  keyword      = {hospital sewage,oxytetracycline resistance,freshwater fishfarm,ANTIBIOTIC-RESISTANCE,PRODUCTION PLANTS,AEROMONAS,DIVERSITY,EFFLUENT,SAMPLES,RIVERS},
  language     = {eng},
  number       = {1},
  pages        = {122--130},
  title        = {Comparison of the antimicrobial tolerance of oxytetracycline-resistant heterotrophic bacteria isolated from hospital sewage and freshwater fishfarm water in Belgium},
  url          = {http://dx.doi.org/10.1078/0723-2020-00008},
  volume       = {24},
  year         = {2001},
}

Chicago
Huys, Geert, Dirk Gevers, Robin Temmerman, Margo Cnockaert, Rik Denys, Glenn Rhodes, Roger Pickup, et al. 2001. “Comparison of the Antimicrobial Tolerance of Oxytetracycline-resistant Heterotrophic Bacteria Isolated from Hospital Sewage and Freshwater Fishfarm Water in Belgium.” Systematic and Applied Microbiology 24 (1): 122–130.
APA
Huys, G., Gevers, D., Temmerman, R., Cnockaert, M., Denys, R., Rhodes, G., Pickup, R., et al. (2001). Comparison of the antimicrobial tolerance of oxytetracycline-resistant heterotrophic bacteria isolated from hospital sewage and freshwater fishfarm water in Belgium. SYSTEMATIC AND APPLIED MICROBIOLOGY, 24(1), 122–130.
Vancouver
1.
Huys G, Gevers D, Temmerman R, Cnockaert M, Denys R, Rhodes G, et al. Comparison of the antimicrobial tolerance of oxytetracycline-resistant heterotrophic bacteria isolated from hospital sewage and freshwater fishfarm water in Belgium. SYSTEMATIC AND APPLIED MICROBIOLOGY. 2001;24(1):122–30.
MLA
Huys, Geert, Dirk Gevers, Robin Temmerman, et al. “Comparison of the Antimicrobial Tolerance of Oxytetracycline-resistant Heterotrophic Bacteria Isolated from Hospital Sewage and Freshwater Fishfarm Water in Belgium.” SYSTEMATIC AND APPLIED MICROBIOLOGY 24.1 (2001): 122–130. Print.