Advanced search
1 file | 124.93 KB

Characterisation of the Roundup Ready soybean insert

Author
Organization
Abstract
In this article we describe the isolation and characterisation of the junction between insert DNA and plant DNA in the transgenic Roundup Ready soybean line event 40-3-2. Our results establish that during integration of the insert DNA several rearrangements occurred at the 3' NOS junction and that the genomic plant DNA at the pre-integration site may have been rearranged. These findings highlight the utility of characterising junction regions to fulfil the request for information regarding which DNA sequences have been incorporated in commercialised transgenic lines. Furthermore, the characterisation of junction regions is, in our opinion, the method of choice to support method development for detection and identification of plant biotechnology-derived products.
Keywords
junction fragment, Roundup Ready soybean, rearrangements, GMO, identification, QUANTITATIVE COMPETITIVE PCR, DNA, PLANTS, CORN

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 124.93 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Windels, Pieter, Isabel Taverniers, Anna Depicker, Erik Van Bockstaele, and Marc De Loose. 2001. “Characterisation of the Roundup Ready Soybean Insert.” European Food Research and Technology 213 (2): 107–112.
APA
Windels, Pieter, Taverniers, I., Depicker, A., Van Bockstaele, E., & De Loose, M. (2001). Characterisation of the Roundup Ready soybean insert. EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 213(2), 107–112.
Vancouver
1.
Windels P, Taverniers I, Depicker A, Van Bockstaele E, De Loose M. Characterisation of the Roundup Ready soybean insert. EUROPEAN FOOD RESEARCH AND TECHNOLOGY. 2001;213(2):107–12.
MLA
Windels, Pieter, Isabel Taverniers, Anna Depicker, et al. “Characterisation of the Roundup Ready Soybean Insert.” EUROPEAN FOOD RESEARCH AND TECHNOLOGY 213.2 (2001): 107–112. Print.
@article{138834,
  abstract     = {In this article we describe the isolation and characterisation of the junction between insert DNA and plant DNA in the transgenic Roundup Ready soybean line event 40-3-2. Our results establish that during integration of the insert DNA several rearrangements occurred at the 3' NOS junction and that the genomic plant DNA at the pre-integration site may have been rearranged. These findings highlight the utility of characterising junction regions to fulfil the request for information regarding which DNA sequences have been incorporated in commercialised transgenic lines. Furthermore, the characterisation of junction regions is, in our opinion, the method of choice to support method development for detection and identification of plant biotechnology-derived products.},
  author       = {Windels, Pieter and Taverniers, Isabel and Depicker, Anna and Van Bockstaele, Erik and De Loose, Marc},
  issn         = {1438-2377},
  journal      = {EUROPEAN FOOD RESEARCH AND TECHNOLOGY},
  keywords     = {junction fragment,Roundup Ready soybean,rearrangements,GMO,identification,QUANTITATIVE COMPETITIVE PCR,DNA,PLANTS,CORN},
  language     = {eng},
  number       = {2},
  pages        = {107--112},
  title        = {Characterisation of the Roundup Ready soybean insert},
  url          = {http://dx.doi.org/10.1007/s002170100336},
  volume       = {213},
  year         = {2001},
}

Altmetric
View in Altmetric
Web of Science
Times cited: