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Analysis of cell division parameters and cell cycle gene expression during the cultivation of Arabidopsis thaliana cell suspensions

(2001) JOURNAL OF EXPERIMENTAL BOTANY. 52(361). p.1625-1633
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Abstract
Arabidopsis thaliana cell suspension cultures were characterized for the first time in detail in terms of biomass accumulation, cell division rate and cell cycle phase durations. Subsequently, this model system was used to follow the transcription profile of key cell cycle genes during a complete cultivation cycle. According to the calculated changes in the relative division rate over time, the cell cycle genes could be classified into four groups based on their transcriptional expression pattern. These different al patterns of gene expression are discussed with respect to the putative roles of the different cell cycle genes in the division cycle. Analysis of protein levels showed that mRNA levels did not correlate with protein levels in all cases. Results obtained in other systems, such as BY-2 cell suspensions or plants, confirm that cell suspension cultures of A. thaliana are suitable for the analysis of cell cycle regulation.
Keywords
cell cycle, Arabidopsis thaliana, gene expression, DEPENDENT KINASES, DEVELOPMENTAL EXPRESSION, LEAF EXPANSION, PLANT, LEAVES, INHIBITOR, G1, MERISTEM, PATTERN, FAMILY

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Citation

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Chicago
Richard, Caroline, Christine Granier, Dirk Inzé, and Lieven De Veylder. 2001. “Analysis of Cell Division Parameters and Cell Cycle Gene Expression During the Cultivation of Arabidopsis Thaliana Cell Suspensions.” Journal of Experimental Botany 52 (361): 1625–1633.
APA
Richard, C., Granier, C., Inzé, D., & De Veylder, L. (2001). Analysis of cell division parameters and cell cycle gene expression during the cultivation of Arabidopsis thaliana cell suspensions. JOURNAL OF EXPERIMENTAL BOTANY, 52(361), 1625–1633.
Vancouver
1.
Richard C, Granier C, Inzé D, De Veylder L. Analysis of cell division parameters and cell cycle gene expression during the cultivation of Arabidopsis thaliana cell suspensions. JOURNAL OF EXPERIMENTAL BOTANY. 2001;52(361):1625–33.
MLA
Richard, Caroline, Christine Granier, Dirk Inzé, et al. “Analysis of Cell Division Parameters and Cell Cycle Gene Expression During the Cultivation of Arabidopsis Thaliana Cell Suspensions.” JOURNAL OF EXPERIMENTAL BOTANY 52.361 (2001): 1625–1633. Print.
@article{138760,
  abstract     = {Arabidopsis thaliana cell suspension cultures were characterized for the first time in detail in terms of biomass accumulation, cell division rate and cell cycle phase durations. Subsequently, this model system was used to follow the transcription profile of key cell cycle genes during a complete cultivation cycle. According to the calculated changes in the relative division rate over time, the cell cycle genes could be classified into four groups based on their transcriptional expression pattern. These different al patterns of gene expression are discussed with respect to the putative roles of the different cell cycle genes in the division cycle. Analysis of protein levels showed that mRNA levels did not correlate with protein levels in all cases. Results obtained in other systems, such as BY-2 cell suspensions or plants, confirm that cell suspension cultures of A. thaliana are suitable for the analysis of cell cycle regulation.},
  author       = {Richard, Caroline and Granier, Christine and Inzé, Dirk and De Veylder, Lieven},
  issn         = {0022-0957},
  journal      = {JOURNAL OF EXPERIMENTAL BOTANY},
  keywords     = {cell cycle,Arabidopsis thaliana,gene expression,DEPENDENT KINASES,DEVELOPMENTAL EXPRESSION,LEAF EXPANSION,PLANT,LEAVES,INHIBITOR,G1,MERISTEM,PATTERN,FAMILY},
  language     = {eng},
  number       = {361},
  pages        = {1625--1633},
  title        = {Analysis of cell division parameters and cell cycle gene expression during the cultivation of Arabidopsis thaliana cell suspensions},
  url          = {http://dx.doi.org/10.1093/jexbot/52.361.1625},
  volume       = {52},
  year         = {2001},
}

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