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A plant-specific cyclin-dependent kinase is involved in the control of G₂/M progression in plants

(2001) JOURNAL OF BIOLOGICAL CHEMISTRY. 276(39). p.36354-36360
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Abstract
Cyclin-dependent kinases (CDKs) control the key transitions in the eukaryotic cell cycle. All the CDKs known to control G(2)/M progression in yeast and animals are distinguished by the characteristic PSTAIRE motif in their cyclin-binding domain and are closely related. Higher plants contain in addition a number of more divergent non-PSTAIRE CDKs with still obscure functions. We show that a plant-specific type of non-PSTAIRE CDKs is involved in the control of the G(2)/M progression. In synchronized tobacco BY-2 cells, the corresponding protein, accumulated in a cell cycle-regulated fashion, peaking at the G(2)/M transition. The associated histone HI kinase activity reached a maximum in mitosis and required a yet unidentified subunit to be fully active. Down-regulation of the associated kinase activity in transgenic tobacco plants using a dominant-negative mutation delayed G(2)/M transition. These results provide the first evidence that non-PSTAIRE CDKs are involved in the control of the G(2)/M progression in plants.
Keywords
ORYZA-SATIVA, S-PHASE, CDC2 GENE, ALFALFA CELLS, TRANSGENIC TOBACCO, FUNCTIONAL HOMOLOG, FISSION YEAST, PROTEIN-KINASE, CELL-CYCLE, ARABIDOPSIS-THALIANA

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Citation

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Chicago
Porceddu, Andrea, Hilde Stals, Jean-Philippe Reichheld, Gerda Segers, Lieven De Veylder, Rosa de Pinho Barrôco, Peter Casteels, Marc Van Montagu, Dirk Inzé, and Vladimir Mironov. 2001. “A Plant-specific Cyclin-dependent Kinase Is Involved in the Control of G₂/M Progression in Plants.” Journal of Biological Chemistry 276 (39): 36354–36360.
APA
Porceddu, A., Stals, H., Reichheld, J.-P., Segers, G., De Veylder, L., Barrôco, R. de P., Casteels, P., et al. (2001). A plant-specific cyclin-dependent kinase is involved in the control of G₂/M progression in plants. JOURNAL OF BIOLOGICAL CHEMISTRY, 276(39), 36354–36360.
Vancouver
1.
Porceddu A, Stals H, Reichheld J-P, Segers G, De Veylder L, Barrôco R de P, et al. A plant-specific cyclin-dependent kinase is involved in the control of G₂/M progression in plants. JOURNAL OF BIOLOGICAL CHEMISTRY. 2001;276(39):36354–60.
MLA
Porceddu, Andrea, Hilde Stals, Jean-Philippe Reichheld, et al. “A Plant-specific Cyclin-dependent Kinase Is Involved in the Control of G₂/M Progression in Plants.” JOURNAL OF BIOLOGICAL CHEMISTRY 276.39 (2001): 36354–36360. Print.
@article{138731,
  abstract     = {Cyclin-dependent kinases (CDKs) control the key transitions in the eukaryotic cell cycle. All the CDKs known to control G(2)/M progression in yeast and animals are distinguished by the characteristic PSTAIRE motif in their cyclin-binding domain and are closely related. Higher plants contain in addition a number of more divergent non-PSTAIRE CDKs with still obscure functions. We show that a plant-specific type of non-PSTAIRE CDKs is involved in the control of the G(2)/M progression. In synchronized tobacco BY-2 cells, the corresponding protein, accumulated in a cell cycle-regulated fashion, peaking at the G(2)/M transition. The associated histone HI kinase activity reached a maximum in mitosis and required a yet unidentified subunit to be fully active. Down-regulation of the associated kinase activity in transgenic tobacco plants using a dominant-negative mutation delayed G(2)/M transition. These results provide the first evidence that non-PSTAIRE CDKs are involved in the control of the G(2)/M progression in plants.},
  author       = {Porceddu, Andrea and Stals, Hilde and Reichheld, Jean-Philippe and Segers, Gerda and De Veylder, Lieven and Barr{\^o}co, Rosa de Pinho and Casteels, Peter and Van Montagu, Marc and Inz{\'e}, Dirk and Mironov, Vladimir},
  issn         = {0021-9258},
  journal      = {JOURNAL OF BIOLOGICAL CHEMISTRY},
  keyword      = {ORYZA-SATIVA,S-PHASE,CDC2 GENE,ALFALFA CELLS,TRANSGENIC TOBACCO,FUNCTIONAL HOMOLOG,FISSION YEAST,PROTEIN-KINASE,CELL-CYCLE,ARABIDOPSIS-THALIANA},
  language     = {eng},
  number       = {39},
  pages        = {36354--36360},
  title        = {A plant-specific cyclin-dependent kinase is involved in the control of G\unmatched{2082}/M progression in plants},
  url          = {http://dx.doi.org/10.1074/jbc.M011060200},
  volume       = {276},
  year         = {2001},
}

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