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The att locus of Rhodococcus fascians strain D188 is essential for full virulence on tobacco through the production of an autoregulatory compound

(2001) MOLECULAR MICROBIOLOGY. 42(1). p.13-28
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Abstract
The ability of Rhodococcus fascians strain D188 to provoke leafy gall formation on a variety of plant species is correlated with the linear plasmid pFiD188, on which different pathogenicity loci were identified. The att locus affects the severity of symptom development on tobacco, whereas the fas locus is essential for virulence. To gain insight into the function of the att locus, sequence and expression analyses were performed. The att locus contains nine open reading frames homologous to arginine and beta -lactam biosynthetic genes. aft gene expression is transcriptionally induced by leafy gall extracts, but not by extracts of uninfected plants, and depends on the attR gene that encodes a LysR-type transcriptional regulator. The att locus proves to be essential for the formation of inducing factors (IFs) that are present in gall extracts. Because the induction of the las locus also requires the presence of IFs in gall extracts, the att locus is proposed to play an important role in regulating the expression of the virulence loci of R. fascians.
Keywords
ESCHERICHIA-COLI, TRANSCRIPTIONAL REGULATORS, BETA-LACTAM, LUXR-LUXI FAMILY, CLAVULANIC ACID BIOSYNTHESIS, GRAM-POSITIVE BACTERIA, STREPTOMYCES-CLAVULIGERUS, NUCLEOTIDE-SEQUENCE, LEAFY GALL, GENE

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Citation

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Chicago
Maes, Tania, Danny Vereecke, Tita Ritsema, Karen Cornelis, Hang Ngo Thi Thu, Marc Van Montagu, Marcella Holsters, and Koen Goethals. 2001. “The Att Locus of Rhodococcus Fascians Strain D188 Is Essential for Full Virulence on Tobacco Through the Production of an Autoregulatory Compound.” Molecular Microbiology 42 (1): 13–28.
APA
Maes, Tania, Vereecke, D., Ritsema, T., Cornelis, K., Thu, H. N. T., Van Montagu, M., Holsters, M., et al. (2001). The att locus of Rhodococcus fascians strain D188 is essential for full virulence on tobacco through the production of an autoregulatory compound. MOLECULAR MICROBIOLOGY, 42(1), 13–28.
Vancouver
1.
Maes T, Vereecke D, Ritsema T, Cornelis K, Thu HNT, Van Montagu M, et al. The att locus of Rhodococcus fascians strain D188 is essential for full virulence on tobacco through the production of an autoregulatory compound. MOLECULAR MICROBIOLOGY. 2001;42(1):13–28.
MLA
Maes, Tania, Danny Vereecke, Tita Ritsema, et al. “The Att Locus of Rhodococcus Fascians Strain D188 Is Essential for Full Virulence on Tobacco Through the Production of an Autoregulatory Compound.” MOLECULAR MICROBIOLOGY 42.1 (2001): 13–28. Print.
@article{138681,
  abstract     = {The ability of Rhodococcus fascians strain D188 to provoke leafy gall formation on a variety of plant species is correlated with the linear plasmid pFiD188, on which different pathogenicity loci were identified. The att locus affects the severity of symptom development on tobacco, whereas the fas locus is essential for virulence. To gain insight into the function of the att locus, sequence and expression analyses were performed. The att locus contains nine open reading frames homologous to arginine and beta -lactam biosynthetic genes. aft gene expression is transcriptionally induced by leafy gall extracts, but not by extracts of uninfected plants, and depends on the attR gene that encodes a LysR-type transcriptional regulator. The att locus proves to be essential for the formation of inducing factors (IFs) that are present in gall extracts. Because the induction of the las locus also requires the presence of IFs in gall extracts, the att locus is proposed to play an important role in regulating the expression of the virulence loci of R. fascians.},
  author       = {Maes, Tania and Vereecke, Danny and Ritsema, Tita and Cornelis, Karen and Thu, Hang Ngo Thi and Van Montagu, Marc and Holsters, Marcella and Goethals, Koen},
  issn         = {0950-382X},
  journal      = {MOLECULAR MICROBIOLOGY},
  keyword      = {ESCHERICHIA-COLI,TRANSCRIPTIONAL REGULATORS,BETA-LACTAM,LUXR-LUXI FAMILY,CLAVULANIC ACID BIOSYNTHESIS,GRAM-POSITIVE BACTERIA,STREPTOMYCES-CLAVULIGERUS,NUCLEOTIDE-SEQUENCE,LEAFY GALL,GENE},
  language     = {eng},
  number       = {1},
  pages        = {13--28},
  title        = {The att locus of Rhodococcus fascians strain D188 is essential for full virulence on tobacco through the production of an autoregulatory compound},
  url          = {http://dx.doi.org/10.1046/j.1365-2958.2001.02615.x},
  volume       = {42},
  year         = {2001},
}

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