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Functional analysis of cyclin-dependent kinase inhibitors of Arabidopsis

(2001) PLANT CELL. 13(7). p.1653-1667
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Abstract
Cyclin-dependent kinase inhibitors, such as the mammalian p27(Kip1) protein, regulate correct cell cycle progression and the integration of developmental signals with the core cell cycle machinery. These inhibitors have been described in plants, but their function remains unresolved. We have isolated seven genes from Arabidopsis that encode proteins with distant sequence homology with p27(Kip1), designated Kip-related proteins (KRPs). The KRPs were characterized by their domain organization and transcript profiles, With the exception of KRP5, all presented the same cyclin-dependent kinase binding specificity. When overproduced, KRP2 dramatically inhibited cell cycle progression in leaf primordia cells without affecting the temporal pattern of cell division and differentiation. Mature transgenic leaves were serrated and consisted of enlarged cells. Although the ploidy levels in young leaves were unaffected, endoreduplication was suppressed in older leaves. We conclude that KRP2 exerts a plant growth inhibitory activity by reducing cell proliferation in leaves, but, in contrast to its mammalian counterparts, it may not control the timing of cell cycle exit and differentiation.
Keywords
EXPRESSION, P27(KIP1), PLANTS, PROTEIN, THALIANA, ROOT-GROWTH, CDK INHIBITORS, MEDIATED TRANSFORMATION, CELL-CYCLE, FISSION YEAST

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Chicago
De Veylder, Lieven, Tom Beeckman, Gerrit Beemster, Luc Krols, Franky Terras, Isabelle Landrieu, Els Van Der Schueren, Sara Maes, Mirande Naudts, and Dirk Inzé. 2001. “Functional Analysis of Cyclin-dependent Kinase Inhibitors of Arabidopsis.” Plant Cell 13 (7): 1653–1667.
APA
De Veylder, L., Beeckman, T., Beemster, G., Krols, L., Terras, F., Landrieu, I., Van Der Schueren, E., et al. (2001). Functional analysis of cyclin-dependent kinase inhibitors of Arabidopsis. PLANT CELL, 13(7), 1653–1667.
Vancouver
1.
De Veylder L, Beeckman T, Beemster G, Krols L, Terras F, Landrieu I, et al. Functional analysis of cyclin-dependent kinase inhibitors of Arabidopsis. PLANT CELL. 2001;13(7):1653–67.
MLA
De Veylder, Lieven, Tom Beeckman, Gerrit Beemster, et al. “Functional Analysis of Cyclin-dependent Kinase Inhibitors of Arabidopsis.” PLANT CELL 13.7 (2001): 1653–1667. Print.
@article{138629,
  abstract     = {Cyclin-dependent kinase inhibitors, such as the mammalian p27(Kip1) protein, regulate correct cell cycle progression and the integration of developmental signals with the core cell cycle machinery. These inhibitors have been described in plants, but their function remains unresolved. We have isolated seven genes from Arabidopsis that encode proteins with distant sequence homology with p27(Kip1), designated Kip-related proteins (KRPs). The KRPs were characterized by their domain organization and transcript profiles, With the exception of KRP5, all presented the same cyclin-dependent kinase binding specificity. When overproduced, KRP2 dramatically inhibited cell cycle progression in leaf primordia cells without affecting the temporal pattern of cell division and differentiation. Mature transgenic leaves were serrated and consisted of enlarged cells. Although the ploidy levels in young leaves were unaffected, endoreduplication was suppressed in older leaves. We conclude that KRP2 exerts a plant growth inhibitory activity by reducing cell proliferation in leaves, but, in contrast to its mammalian counterparts, it may not control the timing of cell cycle exit and differentiation.},
  author       = {De Veylder, Lieven and Beeckman, Tom and Beemster, Gerrit and Krols, Luc and Terras, Franky and Landrieu, Isabelle and Van Der Schueren, Els and Maes, Sara and Naudts, Mirande and Inz{\'e}, Dirk},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {EXPRESSION,P27(KIP1),PLANTS,PROTEIN,THALIANA,ROOT-GROWTH,CDK INHIBITORS,MEDIATED TRANSFORMATION,CELL-CYCLE,FISSION YEAST},
  language     = {eng},
  number       = {7},
  pages        = {1653--1667},
  title        = {Functional analysis of cyclin-dependent kinase inhibitors of Arabidopsis},
  url          = {http://dx.doi.org/10.1105/tpc.13.7.1653},
  volume       = {13},
  year         = {2001},
}

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