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Disruption of their palindromic arrangement leads to selective loss of DNA methylation in inversely repeated gus transgenes in Arabidopsis

Sylvie De Buck UGent and Anna Depicker UGent (2001) MOLECULAR GENETICS AND GENOMICS. 265(6). p.1060-1068
abstract
The transgene locus KH15, which is highly susceptible to silencing in Arabidopsis thaliana, contains two inversely repeated beta -glucuronidase (gus) genes separated by a palindromic sequence and has a low GUS activity, was found to be heavily methylated in the gus coding sequence and in the center of the inverted repeat. The locus KHsb67, which is less prone to silencing, was found to be less densely methylated in the non-repetitive region that separates the inversely repeated gus genes. After the removal of one of the gus genes by Cre-mediated recombination, methylation in both loci decreased or was totally lost. Despite the presence of a 732-bp palindromic sequence in the deletion line derived from KH15, this sequence was not methylated. Whereas the KH15 locus triggers methylation of homologous gus genes when placed in trans to them, the deletion derivative did not. suggesting that the capacity for cross-talk was severely affected by disruption of the palindromic arrangement. This result suggests that the transcribed palindromic sequences are required to maintain the methylation of both symmetrically and non-symmetrically arranged cytosines.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DNA methylation, cre/lox, double-stranded RNA, homology-dependent gene silencing, inverted repeat, THALIANA ROOT EXPLANTS, CHALCONE SYNTHASE, CYTOSINE METHYLATION, VIRUS-RESISTANCE, SINGLE-COPY, GENE, PLANTS, EXPRESSION, SEQUENCES, PROMOTER
journal title
MOLECULAR GENETICS AND GENOMICS
Mol. Genet. Genomics
volume
265
issue
6
pages
1060 - 1068
Web of Science type
Article
Web of Science id
000170491400013
ISSN
1617-4615
DOI
10.1007/s004380100503
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
138616
handle
http://hdl.handle.net/1854/LU-138616
date created
2004-01-14 13:37:00
date last changed
2016-12-19 15:38:04
@article{138616,
  abstract     = {The transgene locus KH15, which is highly susceptible to silencing in Arabidopsis thaliana, contains two inversely repeated beta -glucuronidase (gus) genes separated by a palindromic sequence and has a low GUS activity, was found to be heavily methylated in the gus coding sequence and in the center of the inverted repeat. The locus KHsb67, which is less prone to silencing, was found to be less densely methylated in the non-repetitive region that separates the inversely repeated gus genes. After the removal of one of the gus genes by Cre-mediated recombination, methylation in both loci decreased or was totally lost. Despite the presence of a 732-bp palindromic sequence in the deletion line derived from KH15, this sequence was not methylated. Whereas the KH15 locus triggers methylation of homologous gus genes when placed in trans to them, the deletion derivative did not. suggesting that the capacity for cross-talk was severely affected by disruption of the palindromic arrangement. This result suggests that the transcribed palindromic sequences are required to maintain the methylation of both symmetrically and non-symmetrically arranged cytosines.},
  author       = {De Buck, Sylvie and Depicker, Anna},
  issn         = {1617-4615},
  journal      = {MOLECULAR GENETICS AND GENOMICS},
  keyword      = {DNA methylation,cre/lox,double-stranded RNA,homology-dependent gene silencing,inverted repeat,THALIANA ROOT EXPLANTS,CHALCONE SYNTHASE,CYTOSINE METHYLATION,VIRUS-RESISTANCE,SINGLE-COPY,GENE,PLANTS,EXPRESSION,SEQUENCES,PROMOTER},
  language     = {eng},
  number       = {6},
  pages        = {1060--1068},
  title        = {Disruption of their palindromic arrangement leads to selective loss of DNA methylation in inversely repeated gus transgenes in Arabidopsis},
  url          = {http://dx.doi.org/10.1007/s004380100503},
  volume       = {265},
  year         = {2001},
}

Chicago
De Buck, Sylvie, and Anna Depicker. 2001. “Disruption of Their Palindromic Arrangement Leads to Selective Loss of DNA Methylation in Inversely Repeated Gus Transgenes in Arabidopsis.” Molecular Genetics and Genomics 265 (6): 1060–1068.
APA
De Buck, S., & Depicker, A. (2001). Disruption of their palindromic arrangement leads to selective loss of DNA methylation in inversely repeated gus transgenes in Arabidopsis. MOLECULAR GENETICS AND GENOMICS, 265(6), 1060–1068.
Vancouver
1.
De Buck S, Depicker A. Disruption of their palindromic arrangement leads to selective loss of DNA methylation in inversely repeated gus transgenes in Arabidopsis. MOLECULAR GENETICS AND GENOMICS. 2001;265(6):1060–8.
MLA
De Buck, Sylvie, and Anna Depicker. “Disruption of Their Palindromic Arrangement Leads to Selective Loss of DNA Methylation in Inversely Repeated Gus Transgenes in Arabidopsis.” MOLECULAR GENETICS AND GENOMICS 265.6 (2001): 1060–1068. Print.