Advanced search
1 file | 256.68 KB

The syringaldazine-oxidizing peroxidase PXP 3-4 from poplar xylem: cDNA isolation, characterization and expression

(2001) PLANT MOLECULAR BIOLOGY. 47(5). p.581-593
Author
Organization
Abstract
The cell wall polymer lignin is believed to be condensed by specific cell wall-localized oxidoreductases. In many plants species, including poplar, the peroxidase-directed oxidation of the lignin analogue syringaldazine (SYR) has been localized to cells that undergo secondary wall formation, a process that includes lignification. As a first step to analyse the corresponding peroxidases, we have isolated previously two anionic isoenzymes (PXP 3-4 and PXP 5) from poplar xylem (Populus trichocarpa), which use SYR as a substrate. Here, we demonstrate that these enzymes are responsible for the visualized SYR oxidation in the developing xylem. The cDNA that corresponds to PXP 3-4 was isolated and the deduced protein was found closely related to the other SYR-oxidizing peroxidase PXP 5 (ca. 98% of identity). PXP 3-4 was expressed in a baculovirus expression system yielding high levels of active peroxidase (3 mg/l medium). The heterologously produced protein showed characteristics similar to those of the corresponding protein from poplar xylem (enzymatic properties, isoelectric point, and migration in a native gel). PXP 3-4 was expressed in the stem and in the root xylem. The data demonstrate that PXP 3-4 (and/or PXP 5) are present in differentiating xylem, supporting a function in secondary cell wall formation.
Keywords
MOLECULAR-CLONING, SUSPENSION-CULTURES, ANIONIC PEROXIDASE, POPULUS-KITAKAMIENSIS, HORSERADISH-PEROXIDASE, syringaldazine oxidation, secondary cell wall formation, poplar xylem peroxidase, lignification, cDNA isolation, expression analysis, AMINO-ACID, SEQUENCE, LIGNIFICATION, TOBACCO, LIGNIN

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 256.68 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Christensen, Jørgen Holst, Serge Overney, Antje Rohde, Wilson Ardiles Diaz, Guy Bauw, Patrice Simon, Marc Van Montagu, and Wout Boerjan. 2001. “The Syringaldazine-oxidizing Peroxidase PXP 3-4 from Poplar Xylem: cDNA Isolation, Characterization and Expression.” Plant Molecular Biology 47 (5): 581–593.
APA
Christensen, J. H., Overney, S., Rohde, A., Ardiles Diaz, W., Bauw, G., Simon, P., Van Montagu, M., et al. (2001). The syringaldazine-oxidizing peroxidase PXP 3-4 from poplar xylem: cDNA isolation, characterization and expression. PLANT MOLECULAR BIOLOGY, 47(5), 581–593.
Vancouver
1.
Christensen JH, Overney S, Rohde A, Ardiles Diaz W, Bauw G, Simon P, et al. The syringaldazine-oxidizing peroxidase PXP 3-4 from poplar xylem: cDNA isolation, characterization and expression. PLANT MOLECULAR BIOLOGY. 2001;47(5):581–93.
MLA
Christensen, Jørgen Holst, Serge Overney, Antje Rohde, et al. “The Syringaldazine-oxidizing Peroxidase PXP 3-4 from Poplar Xylem: cDNA Isolation, Characterization and Expression.” PLANT MOLECULAR BIOLOGY 47.5 (2001): 581–593. Print.
@article{138599,
  abstract     = {The cell wall polymer lignin is believed to be condensed by specific cell wall-localized oxidoreductases. In many plants species, including poplar, the peroxidase-directed oxidation of the lignin analogue syringaldazine (SYR) has been localized to cells that undergo secondary wall formation, a process that includes lignification. As a first step to analyse the corresponding peroxidases, we have isolated previously two anionic isoenzymes (PXP 3-4 and PXP 5) from poplar xylem (Populus trichocarpa), which use SYR as a substrate. Here, we demonstrate that these enzymes are responsible for the visualized SYR oxidation in the developing xylem. The cDNA that corresponds to PXP 3-4 was isolated and the deduced protein was found closely related to the other SYR-oxidizing peroxidase PXP 5 (ca. 98\% of identity). PXP 3-4 was expressed in a baculovirus expression system yielding high levels of active peroxidase (3 mg/l medium). The heterologously produced protein showed characteristics similar to those of the corresponding protein from poplar xylem (enzymatic properties, isoelectric point, and migration in a native gel). PXP 3-4 was expressed in the stem and in the root xylem. The data demonstrate that PXP 3-4 (and/or PXP 5) are present in differentiating xylem, supporting a function in secondary cell wall formation.},
  author       = {Christensen, J{\o}rgen Holst and Overney, Serge and Rohde, Antje and Ardiles Diaz, Wilson and Bauw, Guy and Simon, Patrice and Van Montagu, Marc and Boerjan, Wout},
  issn         = {0167-4412},
  journal      = {PLANT MOLECULAR BIOLOGY},
  language     = {eng},
  number       = {5},
  pages        = {581--593},
  title        = {The syringaldazine-oxidizing peroxidase PXP 3-4 from poplar xylem: cDNA isolation, characterization and expression},
  url          = {http://dx.doi.org/10.1023/A:1012271729285},
  volume       = {47},
  year         = {2001},
}

Altmetric
View in Altmetric
Web of Science
Times cited: