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Thermographic visualization of cell death in tobacco and Arabidopsis

Laury Chaerle UGent, F De Boever, Marc Van Montagu UGent and Dominique Van Der Straeten UGent (2001) PLANT CELL AND ENVIRONMENT. 24(1). p.15-25
abstract
Pending cell death was visualized by thermographic imaging in bacterio-opsin transgenic tobacco plants, Cell death in these plants was characterized by a complex lesion phenotype, Isolated cell death lesions were preceded by a colocalized thermal effect, as previously observed at sites infected by tobacco mosaic virus (TMV) (Chaerle et al. 1999 Nature Biotechnology 17, 813-816). However, in most cases, a coherent front of higher temperature, trailed by cell death, initiated at the leaf base and expanded over the leaf lamina. In contrast to the homogenous thermal front, cell death was first visible close to the veins, and subsequently appeared as discrete spots on the interveinal tissue, as cell death spread along the veins. Regions with visible cell death had a lower temperature because of wafer evaporation from damaged cells. In analogy with previous observations on the-localized tobacco-TMV interaction (Chaerle et al. 1999), the kinetics of thermographic and continuous gas exchange measurements indicated that stomatal closure preceded tissue collapse, Localized spontaneous cell death could also be presymptomatically visualized in the Arabidopsis lsd2 mutant.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
hypersensitive response, Arabidopsis lsd2 mutant, infrared thermography, salicylic acid, thermal imaging, stomata, programmed cell death, tobacco bacterioopsin transgenics, transpiration, SYSTEMIC ACQUIRED-RESISTANCE, SALICYLIC-ACID, DISEASE RESISTANCE, HYPERSENSITIVE RESPONSE, TRANSGENIC PLANTS, LESION FORMATION, MOSAIC-VIRUS, GUARD-CELLS, EXPRESSION, MUTANTS
journal title
PLANT CELL AND ENVIRONMENT
Plant Cell Environ.
volume
24
issue
1
pages
15 - 25
Web of Science type
Article
Web of Science id
000166794200002
JCR category
PLANT SCIENCES
JCR impact factor
3.296 (2001)
JCR rank
12/134 (2001)
JCR quartile
1 (2001)
ISSN
0140-7791
DOI
10.1046/j.1365-3040.2001.00654.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
138597
handle
http://hdl.handle.net/1854/LU-138597
date created
2004-01-14 13:37:00
date last changed
2013-10-02 10:14:59
@article{138597,
  abstract     = {Pending cell death was visualized by thermographic imaging in bacterio-opsin transgenic tobacco plants, Cell death in these plants was characterized by a complex lesion phenotype, Isolated cell death lesions were preceded by a colocalized thermal effect, as previously observed at sites infected by tobacco mosaic virus (TMV) (Chaerle et al. 1999 Nature Biotechnology 17, 813-816). However, in most cases, a coherent front of higher temperature, trailed by cell death, initiated at the leaf base and expanded over the leaf lamina. In contrast to the homogenous thermal front, cell death was first visible close to the veins, and subsequently appeared as discrete spots on the interveinal tissue, as cell death spread along the veins. Regions with visible cell death had a lower temperature because of wafer evaporation from damaged cells. In analogy with previous observations on the-localized tobacco-TMV interaction (Chaerle et al. 1999), the kinetics of thermographic and continuous gas exchange measurements indicated that stomatal closure preceded tissue collapse, Localized spontaneous cell death could also be presymptomatically visualized in the Arabidopsis lsd2 mutant.},
  author       = {Chaerle, Laury and De Boever, F and Van Montagu, Marc and Van Der Straeten, Dominique},
  issn         = {0140-7791},
  journal      = {PLANT CELL AND ENVIRONMENT},
  keyword      = {hypersensitive response,Arabidopsis lsd2 mutant,infrared thermography,salicylic acid,thermal imaging,stomata,programmed cell death,tobacco bacterioopsin transgenics,transpiration,SYSTEMIC ACQUIRED-RESISTANCE,SALICYLIC-ACID,DISEASE RESISTANCE,HYPERSENSITIVE RESPONSE,TRANSGENIC PLANTS,LESION FORMATION,MOSAIC-VIRUS,GUARD-CELLS,EXPRESSION,MUTANTS},
  language     = {eng},
  number       = {1},
  pages        = {15--25},
  title        = {Thermographic visualization of cell death in tobacco and Arabidopsis},
  url          = {http://dx.doi.org/10.1046/j.1365-3040.2001.00654.x},
  volume       = {24},
  year         = {2001},
}

Chicago
Chaerle, Laury, F De Boever, Marc Van Montagu, and Dominique Van Der Straeten. 2001. “Thermographic Visualization of Cell Death in Tobacco and Arabidopsis.” Plant Cell and Environment 24 (1): 15–25.
APA
Chaerle, L., De Boever, F., Van Montagu, M., & Van Der Straeten, D. (2001). Thermographic visualization of cell death in tobacco and Arabidopsis. PLANT CELL AND ENVIRONMENT, 24(1), 15–25.
Vancouver
1.
Chaerle L, De Boever F, Van Montagu M, Van Der Straeten D. Thermographic visualization of cell death in tobacco and Arabidopsis. PLANT CELL AND ENVIRONMENT. 2001;24(1):15–25.
MLA
Chaerle, Laury, F De Boever, Marc Van Montagu, et al. “Thermographic Visualization of Cell Death in Tobacco and Arabidopsis.” PLANT CELL AND ENVIRONMENT 24.1 (2001): 15–25. Print.