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Use of double-focusing sector field ICP-mass spectrometry in tracer experiments, aiming at the quantification of Mg2+ transport across the intestine of tilapia fish

Günther De Wannemacker, Angela Ronderos, Luc Moens UGent, Frank Vanhaecke UGent, Marcel JC Bijvelds and Zvonimir I Kolar (2001) JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY. 16(6). p.581-586
abstract
Double-focusing sector field inductively coupled plasma mass spectrometry (ICP-MS) was used for the determination of Mg-26/Mg-24 isotope ratios in order to quantify intestinal Mg2+ transport in the fish tilapia (Oreochromis mossambicus). As the sample medium contained substantial amounts of C, Na, P, S, Cl and K, the occurrence of molecular (e.g., C-2(+), C2H+, CN+ and NaH+) and doubly charged (Ca-48(2+)) ions acid tailing of the Na-23(+) ion signal resulted in spectral overlap at low mass resolution. Application of a higher mass resolution (R = 3000), however, permitted accurate determination of ME isotope ratios. The usefulness of cool plasma conditions was also evaluated, but this approach was insufficient to resolve all interferences completely. After careful optimisation of the measurement parameters, it was possible to measure the Mg-26/Mg-24 isotope ratio with a relative standard deviation (RSD) of 0.2-0.5% (n = 10 x 30 s) at R = 3000. From the isotope ratio results obtained, net intestinal Mg2+ fluxes were calculated, providing evidence for secretory Mg2+ transport. Conclusively, the measurement of stable isotope ratios with sector field ICP-MS presents a sophisticated tool for the study of Mg2+ transport processes in biological systems that may provide an effective alternative for the use of radiotracers.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ISOTOPE RATIO MEASUREMENTS, INDUCTIVELY-COUPLED PLASMA, BIOLOGICAL-MATERIALS, MAGNESIUM TRANSPORT, STABLE ISOTOPES, MS, SINGLE, COLON
journal title
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
J. Anal. At. Spectrom.
volume
16
issue
6
pages
581 - 586
Web of Science type
Article
Web of Science id
000169497700007
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
3.305 (2001)
JCR rank
5/67 (2001)
JCR quartile
1 (2001)
ISSN
0267-9477
DOI
10.1039/b101183i
language
English
UGent publication?
yes
classification
A1
id
138362
handle
http://hdl.handle.net/1854/LU-138362
date created
2004-01-14 13:37:00
date last changed
2012-11-14 13:17:28
@article{138362,
  abstract     = {Double-focusing sector field inductively coupled plasma mass spectrometry (ICP-MS) was used for the determination of Mg-26/Mg-24 isotope ratios in order to quantify intestinal Mg2+ transport in the fish tilapia (Oreochromis mossambicus). As the sample medium contained substantial amounts of C, Na, P, S, Cl and K, the occurrence of molecular (e.g., C-2(+), C2H+, CN+ and NaH+) and doubly charged (Ca-48(2+)) ions acid tailing of the Na-23(+) ion signal resulted in spectral overlap at low mass resolution. Application of a higher mass resolution (R = 3000), however, permitted accurate determination of ME isotope ratios. The usefulness of cool plasma conditions was also evaluated, but this approach was insufficient to resolve all interferences completely. After careful optimisation of the measurement parameters, it was possible to measure the Mg-26/Mg-24 isotope ratio with a relative standard deviation (RSD) of 0.2-0.5\% (n = 10 x 30 s) at R = 3000. From the isotope ratio results obtained, net intestinal Mg2+ fluxes were calculated, providing evidence for secretory Mg2+ transport. Conclusively, the measurement of stable isotope ratios with sector field ICP-MS presents a sophisticated tool for the study of Mg2+ transport processes in biological systems that may provide an effective alternative for the use of radiotracers.},
  author       = {De Wannemacker, G{\"u}nther and Ronderos, Angela and Moens, Luc and Vanhaecke, Frank and Bijvelds, Marcel JC and Kolar, Zvonimir I},
  issn         = {0267-9477},
  journal      = {JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY},
  keyword      = {ISOTOPE RATIO MEASUREMENTS,INDUCTIVELY-COUPLED PLASMA,BIOLOGICAL-MATERIALS,MAGNESIUM TRANSPORT,STABLE ISOTOPES,MS,SINGLE,COLON},
  language     = {eng},
  number       = {6},
  pages        = {581--586},
  title        = {Use of double-focusing sector field ICP-mass spectrometry in tracer experiments, aiming at the quantification of Mg2+ transport across the intestine of tilapia fish},
  url          = {http://dx.doi.org/10.1039/b101183i},
  volume       = {16},
  year         = {2001},
}

Chicago
De Wannemacker, Günther, Angela Ronderos, Luc Moens, Frank Vanhaecke, Marcel JC Bijvelds, and Zvonimir I Kolar. 2001. “Use of Double-focusing Sector Field ICP-mass Spectrometry in Tracer Experiments, Aiming at the Quantification of Mg2+ Transport Across the Intestine of Tilapia Fish.” Journal of Analytical Atomic Spectrometry 16 (6): 581–586.
APA
De Wannemacker, G., Ronderos, A., Moens, L., Vanhaecke, F., Bijvelds, M. J., & Kolar, Z. I. (2001). Use of double-focusing sector field ICP-mass spectrometry in tracer experiments, aiming at the quantification of Mg2+ transport across the intestine of tilapia fish. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 16(6), 581–586.
Vancouver
1.
De Wannemacker G, Ronderos A, Moens L, Vanhaecke F, Bijvelds MJ, Kolar ZI. Use of double-focusing sector field ICP-mass spectrometry in tracer experiments, aiming at the quantification of Mg2+ transport across the intestine of tilapia fish. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY. 2001;16(6):581–6.
MLA
De Wannemacker, Günther, Angela Ronderos, Luc Moens, et al. “Use of Double-focusing Sector Field ICP-mass Spectrometry in Tracer Experiments, Aiming at the Quantification of Mg2+ Transport Across the Intestine of Tilapia Fish.” JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY 16.6 (2001): 581–586. Print.