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Application of the DIVA principle to Salmonella Typhimurium vaccines in pigs avoids interference with serosurveillance programmes

Bregje Leyman UGent, Filip Boyen UGent, Alexander Van Parys UGent, Elin Verbrugghe UGent, Freddy Haesebrouck UGent and Frank Pasmans UGent (2011) SafePork, Proceedings.
abstract
Salmonellosis is one of the most important bacterial zoonotic diseases in humans and Salmonella infections are often linked with the consumption of contaminated pork. In order to reduce Salmonella Typhimurium infections in humans, minimization of the Salmonella intake into the food chain is important. Vaccination has been proposed to control Salmonella infections in pigs. However, pigs vaccinated with the current vaccines cannot be discriminated from infected pigs with the lipopolysaccharide (LPS) -based serological tests used in European serosurveillance programmes. We therefore examined which LPS encoding genes of Salmonella Typhimurium can be deleted to allow differentiation of infected and vaccinated pigs, without affecting the vaccine strain’s protective capacity. For this purpose, deletion mutants in Salmonella strain 112910a, used as vaccine strain, were constructed in the LPS encoding genes: ∆rfbA, ∆rfaL, ∆rfaJ, ∆rfaI, ∆rfaG and ∆rfaF. Inoculation of BALB/c mice with the parent strain, ∆rfaL, ∆rfbA or ∆rfaJ strains but not the ∆rfaG, ∆rfaF or ∆rfaI strains protected significantly against subsequent infection with the virulent Salmonella Typhimurium strain NCTC12023. Immunization of piglets with the ∆rfaJ or ∆rfaL mutants resulted in the induction of a serological response lacking detectable antibodies against LPS. This allowed a differentiation between sera from pigs immunized with the ∆rfaJ or ∆rfaL strains and sera from pigs infected with their isogenic wild type strain.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
in
SafePork, Proceedings
pages
4 pages
conference name
SafePork 2011 : 9th international conference on the epidemiology and control of biological, chemical and physical hazards in pigs and pork
conference location
Maastricht, The Netherlands
conference start
2011-06-19
conference end
2011-06-22
language
English
UGent publication?
yes
classification
C1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1339393
handle
http://hdl.handle.net/1854/LU-1339393
date created
2011-06-24 10:19:00
date last changed
2013-06-21 09:39:30
@inproceedings{1339393,
  abstract     = {Salmonellosis is one of the most important bacterial zoonotic diseases in humans and Salmonella infections are often linked with the consumption of contaminated pork. In order to reduce Salmonella Typhimurium infections in humans, minimization of the Salmonella intake into the food chain is important. Vaccination has been proposed to control Salmonella infections in pigs. However, pigs vaccinated with the current vaccines cannot be discriminated from infected pigs with the lipopolysaccharide (LPS) -based serological tests used in European serosurveillance programmes. We therefore examined which LPS encoding genes of Salmonella Typhimurium can be deleted to allow differentiation of infected and vaccinated pigs, without affecting the vaccine strain{\textquoteright}s protective capacity. For this purpose, deletion mutants in Salmonella strain 112910a, used as vaccine strain, were constructed in the LPS encoding genes: \unmatched{2206}rfbA, \unmatched{2206}rfaL, \unmatched{2206}rfaJ, \unmatched{2206}rfaI, \unmatched{2206}rfaG and \unmatched{2206}rfaF. Inoculation of BALB/c mice with the parent strain, \unmatched{2206}rfaL, \unmatched{2206}rfbA or \unmatched{2206}rfaJ strains but not the \unmatched{2206}rfaG, \unmatched{2206}rfaF or \unmatched{2206}rfaI strains protected significantly against subsequent infection with the virulent Salmonella Typhimurium strain NCTC12023. Immunization of piglets with the \unmatched{2206}rfaJ or \unmatched{2206}rfaL mutants resulted in the induction of a serological response lacking detectable antibodies against LPS. This allowed a differentiation between sera from pigs immunized with the \unmatched{2206}rfaJ or \unmatched{2206}rfaL strains and sera from pigs infected with their isogenic wild type strain.},
  author       = {Leyman, Bregje and Boyen, Filip and Van Parys, Alexander and Verbrugghe, Elin and Haesebrouck, Freddy and Pasmans, Frank},
  booktitle    = {SafePork, Proceedings},
  language     = {eng},
  location     = {Maastricht, The Netherlands},
  pages        = {4},
  title        = {Application of the DIVA principle to Salmonella Typhimurium vaccines in pigs avoids interference with serosurveillance programmes},
  year         = {2011},
}

Chicago
Leyman, Bregje, Filip Boyen, Alexander Van Parys, Elin Verbrugghe, Freddy Haesebrouck, and Frank Pasmans. 2011. “Application of the DIVA Principle to Salmonella Typhimurium Vaccines in Pigs Avoids Interference with Serosurveillance Programmes.” In SafePork, Proceedings.
APA
Leyman, B., Boyen, F., Van Parys, A., Verbrugghe, E., Haesebrouck, F., & Pasmans, F. (2011). Application of the DIVA principle to Salmonella Typhimurium vaccines in pigs avoids interference with serosurveillance programmes. SafePork, Proceedings. Presented at the SafePork 2011 : 9th international conference on the epidemiology and control of biological, chemical and physical hazards in pigs and pork.
Vancouver
1.
Leyman B, Boyen F, Van Parys A, Verbrugghe E, Haesebrouck F, Pasmans F. Application of the DIVA principle to Salmonella Typhimurium vaccines in pigs avoids interference with serosurveillance programmes. SafePork, Proceedings. 2011.
MLA
Leyman, Bregje, Filip Boyen, Alexander Van Parys, et al. “Application of the DIVA Principle to Salmonella Typhimurium Vaccines in Pigs Avoids Interference with Serosurveillance Programmes.” SafePork, Proceedings. 2011. Print.