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Influence of a synbiotic mixture consisting of Lactobacillus acidophilus 74-2 and a fructooligosaccharide preparation on the microbial ecology sustained in a simulation of the human intestinal microbial ecosystem (SHIME reactor)

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Abstract
Lactobacillus acidophilus 74-2, which is used in probiotic products, was administered, with fructooligosaccharide in a milk-based product, to the second vessel (duodenum/jejunum) of the SHIME reactor, an in vitro simulation of the human intestinal microbial ecology. The main focus of this study was to monitor the changes of the population density of selected bacterial species in the intestine and the changes of metabolic activities during the supplementation of L. acidophilus and fructooligosaccharide in the SHIME reactor. Interestingly, the addition of L. acidophilus 74-2 with fructooligosaccharide gave rise to an increase of bifidobacteria. Moreover, major positive changes occurred in the production of volatile fatty acids: a strong upward trend was observed especially in the case of butyric acid and propionic acid. Furthermore a noticeable increase of beta-galactosidase activity was monitored, while the activity of beta-glucuronidase, generally considered undesirable, declined.
Keywords
ENZYMES, DIARRHEA, INVITRO, ASSIMILATION, OLIGOSACCHARIDES, CHOLESTEROL, HUMAN GUT BACTERIA, CONTINUOUS CULTURE SYSTEM, FLORA, MUCIN

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MLA
Gmeiner, M, W Kneifel, KD Kulbe, et al. “Influence of a Synbiotic Mixture Consisting of Lactobacillus Acidophilus 74-2 and a Fructooligosaccharide Preparation on the Microbial Ecology Sustained in a Simulation of the Human Intestinal Microbial Ecosystem (SHIME Reactor).” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 53.2 (2000): 219–223. Print.
APA
Gmeiner, M., Kneifel, W., Kulbe, K., Wouters, R., De Boever, P., Nollet, L., & Verstraete, W. (2000). Influence of a synbiotic mixture consisting of Lactobacillus acidophilus 74-2 and a fructooligosaccharide preparation on the microbial ecology sustained in a simulation of the human intestinal microbial ecosystem (SHIME reactor). APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 53(2), 219–223.
Chicago author-date
Gmeiner, M, W Kneifel, KD Kulbe, Roel Wouters, Patrick De Boever, Lode Nollet, and Willy Verstraete. 2000. “Influence of a Synbiotic Mixture Consisting of Lactobacillus Acidophilus 74-2 and a Fructooligosaccharide Preparation on the Microbial Ecology Sustained in a Simulation of the Human Intestinal Microbial Ecosystem (SHIME Reactor).” Applied Microbiology and Biotechnology 53 (2): 219–223.
Chicago author-date (all authors)
Gmeiner, M, W Kneifel, KD Kulbe, Roel Wouters, Patrick De Boever, Lode Nollet, and Willy Verstraete. 2000. “Influence of a Synbiotic Mixture Consisting of Lactobacillus Acidophilus 74-2 and a Fructooligosaccharide Preparation on the Microbial Ecology Sustained in a Simulation of the Human Intestinal Microbial Ecosystem (SHIME Reactor).” Applied Microbiology and Biotechnology 53 (2): 219–223.
Vancouver
1.
Gmeiner M, Kneifel W, Kulbe K, Wouters R, De Boever P, Nollet L, et al. Influence of a synbiotic mixture consisting of Lactobacillus acidophilus 74-2 and a fructooligosaccharide preparation on the microbial ecology sustained in a simulation of the human intestinal microbial ecosystem (SHIME reactor). APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2000;53(2):219–23.
IEEE
[1]
M. Gmeiner et al., “Influence of a synbiotic mixture consisting of Lactobacillus acidophilus 74-2 and a fructooligosaccharide preparation on the microbial ecology sustained in a simulation of the human intestinal microbial ecosystem (SHIME reactor),” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 53, no. 2, pp. 219–223, 2000.
@article{133400,
  abstract     = {Lactobacillus acidophilus 74-2, which is used in probiotic products, was administered, with fructooligosaccharide in a milk-based product, to the second vessel (duodenum/jejunum) of the SHIME reactor, an in vitro simulation of the human intestinal microbial ecology. The main focus of this study was to monitor the changes of the population density of selected bacterial species in the intestine and the changes of metabolic activities during the supplementation of L. acidophilus and fructooligosaccharide in the SHIME reactor. Interestingly, the addition of L. acidophilus 74-2 with fructooligosaccharide gave rise to an increase of bifidobacteria. Moreover, major positive changes occurred in the production of volatile fatty acids: a strong upward trend was observed especially in the case of butyric acid and propionic acid. Furthermore a noticeable increase of beta-galactosidase activity was monitored, while the activity of beta-glucuronidase, generally considered undesirable, declined.},
  author       = {Gmeiner, M and Kneifel, W and Kulbe, KD and Wouters, Roel and De Boever, Patrick and Nollet, Lode and Verstraete, Willy},
  issn         = {0175-7598},
  journal      = {APPLIED MICROBIOLOGY AND BIOTECHNOLOGY},
  keywords     = {ENZYMES,DIARRHEA,INVITRO,ASSIMILATION,OLIGOSACCHARIDES,CHOLESTEROL,HUMAN GUT BACTERIA,CONTINUOUS CULTURE SYSTEM,FLORA,MUCIN},
  language     = {eng},
  number       = {2},
  pages        = {219--223},
  title        = {Influence of a synbiotic mixture consisting of Lactobacillus acidophilus 74-2 and a fructooligosaccharide preparation on the microbial ecology sustained in a simulation of the human intestinal microbial ecosystem (SHIME reactor)},
  url          = {http://dx.doi.org/10.1007/s002530050011},
  volume       = {53},
  year         = {2000},
}

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