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Protective effect of the bile salt hydrolase-active Lactobacillus reuteri against bile salt cytotoxicity

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Abstract
Bacterial bile salt hydrolysis is considered a risk factor for the development of colon cancer because of the risk of forming harmful secondary bile salts after an initial deconjugation step. In this study, the influence of enhanced bacterial bile salt transformation by the bile salt hydrolase-active Lactobacillus reuteri was studied in batch culture using the microbial suspension of the Simulator of the Human Intestinal Microbial Ecosystem; (SHIME), which was supplemented with oxgall at 5 g/l or 30 g/l. Changes in the fermentative capacity of the microbial ecosystem and the (geno)toxic properties of the SHIME supernatants were investigated. Increasing concentrations of oxgall inhibited the fermentation. Transient cell toxicity was observed for samples supplemented with 5 g oxgall/l, while samples with 30 g oxgall/l exhibited toxicity. The results of the haemolysis test suggest that the detrimental effects were probably due to the membrane-damaging effects of bile salts. In all cases, the adverse effects could be counteracted by the addition of 7.5 +/- 0.5 log(10) CFU L. reuteri/ml. Plausible mechanisms for the protective properties of L. reuteri could involve a precipitation of the deconjugated bile salts and a physical binding of bile salts by the bacterium, thereby making the harmful bile salts less bioavailable.
Keywords
LACTIC-ACID BACTERIA, INTESTINAL MICROBIAL ECOSYSTEM, COLON-CANCER, IN-VITRO, CYTOPROTECTION, CARCINOGENESIS, METABOLISM, TOXICITY, STEROIDS, STRAINS

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Citation

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MLA
De Boever, Patrick, Roel Wouters, L Verschaeve, et al. “Protective Effect of the Bile Salt Hydrolase-active Lactobacillus Reuteri Against Bile Salt Cytotoxicity.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 53.6 (2000): 709–714. Print.
APA
De Boever, P., Wouters, R., Verschaeve, L., Berckmans, P., Schoeters, G., & Verstraete, W. (2000). Protective effect of the bile salt hydrolase-active Lactobacillus reuteri against bile salt cytotoxicity. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 53(6), 709–714.
Chicago author-date
De Boever, Patrick, Roel Wouters, L Verschaeve, P Berckmans, G Schoeters, and Willy Verstraete. 2000. “Protective Effect of the Bile Salt Hydrolase-active Lactobacillus Reuteri Against Bile Salt Cytotoxicity.” Applied Microbiology and Biotechnology 53 (6): 709–714.
Chicago author-date (all authors)
De Boever, Patrick, Roel Wouters, L Verschaeve, P Berckmans, G Schoeters, and Willy Verstraete. 2000. “Protective Effect of the Bile Salt Hydrolase-active Lactobacillus Reuteri Against Bile Salt Cytotoxicity.” Applied Microbiology and Biotechnology 53 (6): 709–714.
Vancouver
1.
De Boever P, Wouters R, Verschaeve L, Berckmans P, Schoeters G, Verstraete W. Protective effect of the bile salt hydrolase-active Lactobacillus reuteri against bile salt cytotoxicity. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2000;53(6):709–14.
IEEE
[1]
P. De Boever, R. Wouters, L. Verschaeve, P. Berckmans, G. Schoeters, and W. Verstraete, “Protective effect of the bile salt hydrolase-active Lactobacillus reuteri against bile salt cytotoxicity,” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 53, no. 6, pp. 709–714, 2000.
@article{133392,
  abstract     = {Bacterial bile salt hydrolysis is considered a risk factor for the development of colon cancer because of the risk of forming harmful secondary bile salts after an initial deconjugation step. In this study, the influence of enhanced bacterial bile salt transformation by the bile salt hydrolase-active Lactobacillus reuteri was studied in batch culture using the microbial suspension of the Simulator of the Human Intestinal Microbial Ecosystem; (SHIME), which was supplemented with oxgall at 5 g/l or 30 g/l. Changes in the fermentative capacity of the microbial ecosystem and the (geno)toxic properties of the SHIME supernatants were investigated. Increasing concentrations of oxgall inhibited the fermentation. Transient cell toxicity was observed for samples supplemented with 5 g oxgall/l, while samples with 30 g oxgall/l exhibited toxicity. The results of the haemolysis test suggest that the detrimental effects were probably due to the membrane-damaging effects of bile salts. In all cases, the adverse effects could be counteracted by the addition of 7.5 +/- 0.5 log(10) CFU L. reuteri/ml. Plausible mechanisms for the protective properties of L. reuteri could involve a precipitation of the deconjugated bile salts and a physical binding of bile salts by the bacterium, thereby making the harmful bile salts less bioavailable.},
  author       = {De Boever, Patrick and Wouters, Roel and Verschaeve, L and Berckmans, P and Schoeters, G and Verstraete, Willy},
  issn         = {0175-7598},
  journal      = {APPLIED MICROBIOLOGY AND BIOTECHNOLOGY},
  keywords     = {LACTIC-ACID BACTERIA,INTESTINAL MICROBIAL ECOSYSTEM,COLON-CANCER,IN-VITRO,CYTOPROTECTION,CARCINOGENESIS,METABOLISM,TOXICITY,STEROIDS,STRAINS},
  language     = {eng},
  number       = {6},
  pages        = {709--714},
  title        = {Protective effect of the bile salt hydrolase-active Lactobacillus reuteri against bile salt cytotoxicity},
  url          = {http://dx.doi.org/10.1007/s002530000330},
  volume       = {53},
  year         = {2000},
}

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