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Induction and conversion of somatic embryogenesis on the anther filament of Spathiphyllum Schott

Stefaan Werbrouck UGent, Tom Eeckhaut and Pierre Debergh (2000) Acta Horticulturae. 520. p.263-269
abstract
A prerequisite for genetic manipulation, mutation breeding or ploidy manipulation of the ornamental crop Spathiphyllum is the availability of a system which allows a target cell to regenerate into a new plant. Induction of somatic embryos could offer a powerful tool to reach this goal. When placed on a Murashige sr Skoog medium supplemented with 10 mu M naphthaleneacetic acid (NAA) and 0.2 - 3 mu M thidiazuron (TDZ), anthers of different genotypes develop clumps of attached somatic embryos on the filament. The younger the anthers, the more sensitive their filament cells were for the induction of somatic embryogenesis. After six weeks, the somatic embryos were transferred to a Murashige & Skoog (MS) medium supplemented with 10 CIM benzylaminopurine (BA) + 0.02 mu M NAA to develop secondary somatic embryos. Another 6 weeks later, they were ready for conversion. The best conversion medium was a hormone-free MS medium. Addition of abscisic acid (ABA) had a negative effect. After a conversion period of 10 weeks, the normal looking plants were transferred to the greenhouse.
Please use this url to cite or link to this publication:
author
organization
year
type
conference (proceedingsPaper)
publication status
published
subject
keyword
Araceae, CULTURE, Spathiphyllum, conversion, embryogenesis, PLANT-REGENERATION, HIPPOCASTANUM L, HISTOLOGY, EMBRYOS, ORIGIN, TISSUE
in
Acta Horticulturae
Acta Hortic.
editor
LHW van der Plas and G-J de Klerk
volume
520
issue title
Proceedings of the XXV international horticultural congress, part 10 : application of biotechnology and molecular biology and breeding : in vitro culture
pages
263 - 269
publisher
International Society for Horticultural Science (ISHS)
place of publication
Leuven, Belgium
conference name
25th International Horticultural Congress
conference location
Brussels, Belgium
conference start
1998-08-02
conference end
1998-08-07
Web of Science type
Proceedings Paper
Web of Science id
000088536200027
ISSN
0567-7572
ISBN
9789066058439
language
English
UGent publication?
yes
classification
P1
id
133085
handle
http://hdl.handle.net/1854/LU-133085
alternative location
http://www.actahort.org/books/520/520_27.htm
date created
2004-01-14 13:36:00
date last changed
2017-03-02 09:42:48
@inproceedings{133085,
  abstract     = {A prerequisite for genetic manipulation, mutation breeding or ploidy manipulation of the ornamental crop Spathiphyllum is the availability of a system which allows a target cell to regenerate into a new plant. Induction of somatic embryos could offer a powerful tool to reach this goal. When placed on a Murashige sr Skoog medium supplemented with 10 mu M naphthaleneacetic acid (NAA) and 0.2 - 3 mu M thidiazuron (TDZ), anthers of different genotypes develop clumps of attached somatic embryos on the filament. The younger the anthers, the more sensitive their filament cells were for the induction of somatic embryogenesis. After six weeks, the somatic embryos were transferred to a Murashige \& Skoog (MS) medium supplemented with 10 CIM benzylaminopurine (BA) + 0.02 mu M NAA to develop secondary somatic embryos. Another 6 weeks later, they were ready for conversion. The best conversion medium was a hormone-free MS medium. Addition of abscisic acid (ABA) had a negative effect. After a conversion period of 10 weeks, the normal looking plants were transferred to the greenhouse.},
  author       = {Werbrouck, Stefaan and Eeckhaut, Tom and Debergh, Pierre},
  booktitle    = {Acta Horticulturae},
  editor       = {van der Plas, LHW and de Klerk, G-J},
  isbn         = {9789066058439},
  issn         = {0567-7572},
  keyword      = {Araceae,CULTURE,Spathiphyllum,conversion,embryogenesis,PLANT-REGENERATION,HIPPOCASTANUM L,HISTOLOGY,EMBRYOS,ORIGIN,TISSUE},
  language     = {eng},
  location     = {Brussels, Belgium},
  pages        = {263--269},
  publisher    = {International Society for Horticultural Science (ISHS)},
  title        = {Induction and conversion of somatic embryogenesis on the anther filament of Spathiphyllum Schott},
  url          = {http://www.actahort.org/books/520/520\_27.htm},
  volume       = {520},
  year         = {2000},
}

Chicago
Werbrouck, Stefaan, Tom Eeckhaut, and Pierre Debergh. 2000. “Induction and Conversion of Somatic Embryogenesis on the Anther Filament of Spathiphyllum Schott.” In Acta Horticulturae, ed. LHW van der Plas and G-J de Klerk, 520:263–269. Leuven, Belgium: International Society for Horticultural Science (ISHS).
APA
Werbrouck, Stefaan, Eeckhaut, T., & Debergh, P. (2000). Induction and conversion of somatic embryogenesis on the anther filament of Spathiphyllum Schott. In L. van der Plas & G.-J. de Klerk (Eds.), Acta Horticulturae (Vol. 520, pp. 263–269). Presented at the 25th International Horticultural Congress, Leuven, Belgium: International Society for Horticultural Science (ISHS).
Vancouver
1.
Werbrouck S, Eeckhaut T, Debergh P. Induction and conversion of somatic embryogenesis on the anther filament of Spathiphyllum Schott. In: van der Plas L, de Klerk G-J, editors. Acta Horticulturae. Leuven, Belgium: International Society for Horticultural Science (ISHS); 2000. p. 263–9.
MLA
Werbrouck, Stefaan, Tom Eeckhaut, and Pierre Debergh. “Induction and Conversion of Somatic Embryogenesis on the Anther Filament of Spathiphyllum Schott.” Acta Horticulturae. Ed. LHW van der Plas & G-J de Klerk. Vol. 520. Leuven, Belgium: International Society for Horticultural Science (ISHS), 2000. 263–269. Print.