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Induction and conversion of somatic embryogenesis on the anther filament of Spathiphyllum Schott

(2000) Acta Horticulturae. 520. p.263-269
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Abstract
A prerequisite for genetic manipulation, mutation breeding or ploidy manipulation of the ornamental crop Spathiphyllum is the availability of a system which allows a target cell to regenerate into a new plant. Induction of somatic embryos could offer a powerful tool to reach this goal. When placed on a Murashige sr Skoog medium supplemented with 10 mu M naphthaleneacetic acid (NAA) and 0.2 - 3 mu M thidiazuron (TDZ), anthers of different genotypes develop clumps of attached somatic embryos on the filament. The younger the anthers, the more sensitive their filament cells were for the induction of somatic embryogenesis. After six weeks, the somatic embryos were transferred to a Murashige & Skoog (MS) medium supplemented with 10 CIM benzylaminopurine (BA) + 0.02 mu M NAA to develop secondary somatic embryos. Another 6 weeks later, they were ready for conversion. The best conversion medium was a hormone-free MS medium. Addition of abscisic acid (ABA) had a negative effect. After a conversion period of 10 weeks, the normal looking plants were transferred to the greenhouse.
Keywords
Araceae, CULTURE, Spathiphyllum, conversion, embryogenesis, PLANT-REGENERATION, HIPPOCASTANUM L, HISTOLOGY, EMBRYOS, ORIGIN, TISSUE

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Chicago
Werbrouck, Stefaan, Tom Eeckhaut, and Pierre Debergh. 2000. “Induction and Conversion of Somatic Embryogenesis on the Anther Filament of Spathiphyllum Schott.” In Acta Horticulturae, ed. LHW van der Plas and G-J de Klerk, 520:263–269. Leuven, Belgium: International Society for Horticultural Science (ISHS).
APA
Werbrouck, Stefaan, Eeckhaut, T., & Debergh, P. (2000). Induction and conversion of somatic embryogenesis on the anther filament of Spathiphyllum Schott. In L. van der Plas & G.-J. de Klerk (Eds.), Acta Horticulturae (Vol. 520, pp. 263–269). Presented at the 25th International Horticultural Congress, Leuven, Belgium: International Society for Horticultural Science (ISHS).
Vancouver
1.
Werbrouck S, Eeckhaut T, Debergh P. Induction and conversion of somatic embryogenesis on the anther filament of Spathiphyllum Schott. In: van der Plas L, de Klerk G-J, editors. Acta Horticulturae. Leuven, Belgium: International Society for Horticultural Science (ISHS); 2000. p. 263–9.
MLA
Werbrouck, Stefaan, Tom Eeckhaut, and Pierre Debergh. “Induction and Conversion of Somatic Embryogenesis on the Anther Filament of Spathiphyllum Schott.” Acta Horticulturae. Ed. LHW van der Plas & G-J de Klerk. Vol. 520. Leuven, Belgium: International Society for Horticultural Science (ISHS), 2000. 263–269. Print.
@inproceedings{133085,
  abstract     = {A prerequisite for genetic manipulation, mutation breeding or ploidy manipulation of the ornamental crop Spathiphyllum is the availability of a system which allows a target cell to regenerate into a new plant. Induction of somatic embryos could offer a powerful tool to reach this goal. When placed on a Murashige sr Skoog medium supplemented with 10 mu M naphthaleneacetic acid (NAA) and 0.2 - 3 mu M thidiazuron (TDZ), anthers of different genotypes develop clumps of attached somatic embryos on the filament. The younger the anthers, the more sensitive their filament cells were for the induction of somatic embryogenesis. After six weeks, the somatic embryos were transferred to a Murashige \& Skoog (MS) medium supplemented with 10 CIM benzylaminopurine (BA) + 0.02 mu M NAA to develop secondary somatic embryos. Another 6 weeks later, they were ready for conversion. The best conversion medium was a hormone-free MS medium. Addition of abscisic acid (ABA) had a negative effect. After a conversion period of 10 weeks, the normal looking plants were transferred to the greenhouse.},
  author       = {Werbrouck, Stefaan and Eeckhaut, Tom and Debergh, Pierre},
  booktitle    = {Acta Horticulturae},
  editor       = {van der Plas, LHW and de Klerk, G-J},
  isbn         = {9789066058439},
  issn         = {0567-7572},
  keyword      = {Araceae,CULTURE,Spathiphyllum,conversion,embryogenesis,PLANT-REGENERATION,HIPPOCASTANUM L,HISTOLOGY,EMBRYOS,ORIGIN,TISSUE},
  language     = {eng},
  location     = {Brussels, Belgium},
  pages        = {263--269},
  publisher    = {International Society for Horticultural Science (ISHS)},
  title        = {Induction and conversion of somatic embryogenesis on the anther filament of Spathiphyllum Schott},
  url          = {http://www.actahort.org/books/520/520\_27.htm},
  volume       = {520},
  year         = {2000},
}

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