Advanced search
1 file | 365.32 KB Add to list

Structural aspects of the zona pellucida of in vitro-produced bovine embryos: a scanning electron and confocal laser scanning microscopic study

(2000) BIOLOGY OF REPRODUCTION. 62(2). p.463-469
Author
Organization
Abstract
Structural aspects of the bovine zona pellucida (ZP) of in vitro-matured (IVM) oocytes and in vitro-produced (IVP) embryos were studied in two experiments to find a tentative explanation for the zona's barrier function against viral infection. In Experiment 1, the ultrastructure of the outer ZP surface was studied. The diameter (nm) and the number of the outer pores within an area of 5000 mu m(2) of 10 IVM oocytes, 10 zygotes, 10 8-cell-stage embryos, and 10 morulae were evaluated by scanning electron microscopy. In oocytes and morulae, the ZP surface showed a rough and spongy appearance with numerous pores. In zygotes, the ZP surface was found to have a smooth, melted appearance with only a few pores; In 8-cell-stage embryos, both surface patterns were found. The mean number (per 5000 mu m(2)) and the mean diameter of the outer pores were different between the four stages of development (P < 0.001): 1511 pores in oocytes, 1187 in zygotes, 1658 in 8-cell-stage embryos, and 3259 in morulae, with mean diameters of 182, 223, 203, and 155 nm, respectively. In Experiment 2, the continuity of the meshes (network of pores) towards the embryonic cells was examined by confocal laser scanning microscopy. Therefore, the passage through and the location in the ZP of fluorescent microspheres, with similar dimensions as bovine viral diarrhea virus (BVDV, 40-50 nm) and bovine herpesvirus-l (BHV-1; 180-200 nm), were evaluated. For all stages, the smallest beads were detected halfway through the thickness of the ZP, whereas the beads with a size of 200 nm were found only within the outer-fourth part of the ZP. It can be concluded that the intact ZP of bovine IVM oocytes and IVP embryos are constructed in such a way that BVDV and BHV-1 should not be able to traverse the ZP and reach the embryonic cells. However, the risk exists that viral particles can be trapped in the outer layers of the ZP.
Keywords
PORCINE PARVOVIRUS, IN-VITRO, HUMAN OOCYTES, INVITRO, IDENTIFICATION, ASSOCIATION, MATURATION, LIVESTOCK, BIOLOGY, INTACT

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 365.32 KB

Citation

Please use this url to cite or link to this publication:

MLA
Vanroose, Geert, et al. “Structural Aspects of the Zona Pellucida of in Vitro-Produced Bovine Embryos: A Scanning Electron and Confocal Laser Scanning Microscopic Study.” BIOLOGY OF REPRODUCTION, vol. 62, no. 2, 2000, pp. 463–69, doi:10.1095/​biolreprod62.2.463.
APA
Vanroose, G., Nauwynck, H., Van Soom, A., Ysebaert, M.-T., Charlier, G., Van Oostveldt, P., & de Kruif, A. (2000). Structural aspects of the zona pellucida of in vitro-produced bovine embryos: a scanning electron and confocal laser scanning microscopic study. BIOLOGY OF REPRODUCTION, 62(2), 463–469. https://doi.org/10.1095/​biolreprod62.2.463
Chicago author-date
Vanroose, Geert, Hans Nauwynck, Ann Van Soom, Marie-Thérèse Ysebaert, Gerard Charlier, Patric Van Oostveldt, and Aart de Kruif. 2000. “Structural Aspects of the Zona Pellucida of in Vitro-Produced Bovine Embryos: A Scanning Electron and Confocal Laser Scanning Microscopic Study.” BIOLOGY OF REPRODUCTION 62 (2): 463–69. https://doi.org/10.1095/​biolreprod62.2.463.
Chicago author-date (all authors)
Vanroose, Geert, Hans Nauwynck, Ann Van Soom, Marie-Thérèse Ysebaert, Gerard Charlier, Patric Van Oostveldt, and Aart de Kruif. 2000. “Structural Aspects of the Zona Pellucida of in Vitro-Produced Bovine Embryos: A Scanning Electron and Confocal Laser Scanning Microscopic Study.” BIOLOGY OF REPRODUCTION 62 (2): 463–469. doi:10.1095/​biolreprod62.2.463.
Vancouver
1.
Vanroose G, Nauwynck H, Van Soom A, Ysebaert M-T, Charlier G, Van Oostveldt P, et al. Structural aspects of the zona pellucida of in vitro-produced bovine embryos: a scanning electron and confocal laser scanning microscopic study. BIOLOGY OF REPRODUCTION. 2000;62(2):463–9.
IEEE
[1]
G. Vanroose et al., “Structural aspects of the zona pellucida of in vitro-produced bovine embryos: a scanning electron and confocal laser scanning microscopic study,” BIOLOGY OF REPRODUCTION, vol. 62, no. 2, pp. 463–469, 2000.
@article{131757,
  abstract     = {{Structural aspects of the bovine zona pellucida (ZP) of in vitro-matured (IVM) oocytes and in vitro-produced (IVP) embryos were studied in two experiments to find a tentative explanation for the zona's barrier function against viral infection.
In Experiment 1, the ultrastructure of the outer ZP surface was studied. The diameter (nm) and the number of the outer pores within an area of 5000 mu m(2) of 10 IVM oocytes, 10 zygotes, 10 8-cell-stage embryos, and 10 morulae were evaluated by scanning electron microscopy. In oocytes and morulae, the ZP surface showed a rough and spongy appearance with numerous pores. In zygotes, the ZP surface was found to have a smooth, melted appearance with only a few pores; In 8-cell-stage embryos, both surface patterns were found. The mean number (per 5000 mu m(2)) and the mean diameter of the outer pores were different between the four stages of development (P < 0.001): 1511 pores in oocytes, 1187 in zygotes, 1658 in 8-cell-stage embryos, and 3259 in morulae, with mean diameters of 182, 223, 203, and 155 nm, respectively.
In Experiment 2, the continuity of the meshes (network of pores) towards the embryonic cells was examined by confocal laser scanning microscopy. Therefore, the passage through and the location in the ZP of fluorescent microspheres, with similar dimensions as bovine viral diarrhea virus (BVDV, 40-50 nm) and bovine herpesvirus-l (BHV-1; 180-200 nm), were evaluated. For all stages, the smallest beads were detected halfway through the thickness of the ZP, whereas the beads with a size of 200 nm were found only within the outer-fourth part of the ZP. It can be concluded that the intact ZP of bovine IVM oocytes and IVP embryos are constructed in such a way that BVDV and BHV-1 should not be able to traverse the ZP and reach the embryonic cells. However, the risk exists that viral particles can be trapped in the outer layers of the ZP.}},
  author       = {{Vanroose, Geert and Nauwynck, Hans and Van Soom, Ann and Ysebaert, Marie-Thérèse and Charlier, Gerard and Van Oostveldt, Patric and de Kruif, Aart}},
  issn         = {{0006-3363}},
  journal      = {{BIOLOGY OF REPRODUCTION}},
  keywords     = {{PORCINE PARVOVIRUS,IN-VITRO,HUMAN OOCYTES,INVITRO,IDENTIFICATION,ASSOCIATION,MATURATION,LIVESTOCK,BIOLOGY,INTACT}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{463--469}},
  title        = {{Structural aspects of the zona pellucida of in vitro-produced bovine embryos: a scanning electron and confocal laser scanning microscopic study}},
  url          = {{http://doi.org/10.1095/​biolreprod62.2.463}},
  volume       = {{62}},
  year         = {{2000}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: