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Loss of vtx genes after the first subcultivation step of verocytotoxigenic Escherichia coli O157 and Non-O157 during isolation from naturally contaminated fecal samples

Adelheid Joris UGent, Karen Verstraete, Koen De Reu and Lieven De Zutter UGent (2011) TOXINS. 3(6). p.672-677
abstract
Verocytotoxins VT1 and VT2, produced by Verocytotoxigenic Escherichia coli (VTEC), are encoded on temperate bacteriophages. Several studies reported the loss of the vtx genes after multiple subcultivation steps or long preservation. The objective of this study was to determine if the loss of the verocytotoxin genes can already occur during the first subcultivation step. Consequently, the stability of the vtx genes were tested in 40 isolates originating from 40 vtx-positive fecal samples after the first subcultivation step following the isolation procedure. The loss occurred in 12 out of 40 strains tested and was rather rare among the O157 strains compared to the non-O157 strains. This is the first study demonstrating that the loss of the verocytotoxin genes can already occur after the first subcultivation step. This may lead to an underestimation of VTEC positive samples.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (letterNote)
publication status
published
subject
keyword
Escherichia coli, VTEC, vtx genes, loss
journal title
TOXINS
Toxins
volume
3
issue
6
pages
672 - 677
ISSN
2072-6651
DOI
10.3390/toxins3060672
language
English
UGent publication?
yes
classification
A2
copyright statement
I have retained and own the full copyright for this publication
id
1268654
handle
http://hdl.handle.net/1854/LU-1268654
date created
2011-06-20 14:05:05
date last changed
2011-06-28 12:04:43
@article{1268654,
  abstract     = {Verocytotoxins VT1 and VT2, produced by Verocytotoxigenic Escherichia coli (VTEC), are encoded on temperate bacteriophages. Several studies reported the loss of the vtx genes after multiple subcultivation steps or long preservation. The objective of this study was to determine if the loss of the verocytotoxin genes can already occur during the first subcultivation step. Consequently, the stability of the vtx genes were tested in 40 isolates originating from 40 vtx-positive fecal samples after the first subcultivation step following the isolation procedure. The loss occurred in 12 out of 40 strains tested and was rather rare among the O157 strains compared to the non-O157 strains. This is the first study demonstrating that the loss of the verocytotoxin genes can already occur after the first subcultivation step. This may lead to an underestimation of VTEC positive samples.},
  author       = {Joris, Adelheid and Verstraete, Karen  and De Reu, Koen  and De Zutter, Lieven},
  issn         = {2072-6651},
  journal      = {TOXINS},
  keyword      = {Escherichia coli,VTEC,vtx genes,loss},
  language     = {eng},
  number       = {6},
  pages        = {672--677},
  title        = {Loss of vtx genes after the first subcultivation step of verocytotoxigenic Escherichia coli O157 and Non-O157 during isolation from naturally contaminated fecal samples},
  url          = {http://dx.doi.org/10.3390/toxins3060672},
  volume       = {3},
  year         = {2011},
}

Chicago
Joris, Adelheid, Karen Verstraete, Koen De Reu, and Lieven De Zutter. 2011. “Loss of Vtx Genes After the First Subcultivation Step of Verocytotoxigenic Escherichia Coli O157 and Non-O157 During Isolation from Naturally Contaminated Fecal Samples.” Toxins 3 (6): 672–677.
APA
Joris, A., Verstraete, K., De Reu, K., & De Zutter, L. (2011). Loss of vtx genes after the first subcultivation step of verocytotoxigenic Escherichia coli O157 and Non-O157 during isolation from naturally contaminated fecal samples. TOXINS, 3(6), 672–677.
Vancouver
1.
Joris A, Verstraete K, De Reu K, De Zutter L. Loss of vtx genes after the first subcultivation step of verocytotoxigenic Escherichia coli O157 and Non-O157 during isolation from naturally contaminated fecal samples. TOXINS. 2011;3(6):672–7.
MLA
Joris, Adelheid, Karen Verstraete, Koen De Reu, et al. “Loss of Vtx Genes After the First Subcultivation Step of Verocytotoxigenic Escherichia Coli O157 and Non-O157 During Isolation from Naturally Contaminated Fecal Samples.” TOXINS 3.6 (2011): 672–677. Print.