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Peroxisomes in zebrafish: distribution pattern and knockdown studies

Olga Krysko UGent, Mieke Stevens UGent, Tobias Langenberg, Marc Fransen, Marc Espeel UGent and Myriam Baes (2010) HISTOCHEMISTRY AND CELL BIOLOGY. 134(1). p.39-51
abstract
Peroxisomes are organelles that are essential for normal development in men and mice. In order to explore whether zebrafish could be used as a model system to study the role of peroxisomes, we examined their distribution pattern in developing and adult zebrafish and we tested different approaches to eliminate them during the first days after fertilization. In 4-day-old embryos, catalase-containing peroxisomes were obvious in the liver, the pronephric duct and the wall of the yolk sac, but transcripts for peroxisomal matrix and membrane proteins were also detected in the head region from 24 h post-fertilization. In adult zebrafish, catalase-containing peroxisomes remained prominent in the hepatocytes, the renal proximal tubules and the intestinal epithelium. Several peroxins, essential proteins for the biogenesis of peroxisomes, were targeted using knockdown approaches. Two morpholinos, blocking, respectively, splice sites in pex3 and pex13, only induced a short in frame deletion or insertion in the transcripts and did not result in the elimination of peroxisomes after injection into one-cell embryos. A morpholino blocking translation of pex13 was able to reduce the number of peroxisomes to variable extents. Finally, overexpression of a potential dominant negative fragment of Pex3p did not result in deletion of peroxisomes from developing zebrafish. We conclude that in zebrafish (1) peroxisomes, as visualized by DAB cytochemistry for catalase activity, are most conspicuous in the liver and renal tubular epithelium; this pattern is reminiscent of peroxisome occurrence in mammalian organs, (2) our approaches to eliminate these organelles during development by targeting peroxins were not successful.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Peroxisomes, Morpholino, Zebrafish, Zellweger syndrome, DANIO-RERIO, IMPORT RECEPTOR, DIGESTIVE-SYSTEM, BRACHYDANIO-RERIO, ZELLWEGER-SYNDROME, KIDNEY DEVELOPMENT, MEMBRANE-PROTEINS, BIOGENESIS DISORDERS, NEMATODE CAENORHABDITIS-ELEGANS, PROLIFERATOR-ACTIVATED RECEPTORS
journal title
HISTOCHEMISTRY AND CELL BIOLOGY
Histochem. Cell Biol.
volume
134
issue
1
pages
39 - 51
Web of Science type
Article
Web of Science id
000279195600005
JCR category
MICROSCOPY
JCR impact factor
4.727 (2010)
JCR rank
1/9 (2010)
JCR quartile
1 (2010)
ISSN
0948-6143
DOI
10.1007/s00418-010-0712-z
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1268652
handle
http://hdl.handle.net/1854/LU-1268652
date created
2011-06-20 14:04:25
date last changed
2011-06-20 15:36:21
@article{1268652,
  abstract     = {Peroxisomes are organelles that are essential for normal development in men and mice. In order to explore whether zebrafish could be used as a model system to study the role of peroxisomes, we examined their distribution pattern in developing and adult zebrafish and we tested different approaches to eliminate them during the first days after fertilization. In 4-day-old embryos, catalase-containing peroxisomes were obvious in the liver, the pronephric duct and the wall of the yolk sac, but transcripts for peroxisomal matrix and membrane proteins were also detected in the head region from 24 h post-fertilization. In adult zebrafish, catalase-containing peroxisomes remained prominent in the hepatocytes, the renal proximal tubules and the intestinal epithelium. Several peroxins, essential proteins for the biogenesis of peroxisomes, were targeted using knockdown approaches. Two morpholinos, blocking, respectively, splice sites in pex3 and pex13, only induced a short in frame deletion or insertion in the transcripts and did not result in the elimination of peroxisomes after injection into one-cell embryos. A morpholino blocking translation of pex13 was able to reduce the number of peroxisomes to variable extents. Finally, overexpression of a potential dominant negative fragment of Pex3p did not result in deletion of peroxisomes from developing zebrafish. We conclude that in zebrafish (1) peroxisomes, as visualized by DAB cytochemistry for catalase activity, are most conspicuous in the liver and renal tubular epithelium; this pattern is reminiscent of peroxisome occurrence in mammalian organs, (2) our approaches to eliminate these organelles during development by targeting peroxins were not successful.},
  author       = {Krysko, Olga and Stevens, Mieke and Langenberg, Tobias and Fransen, Marc and Espeel, Marc and Baes, Myriam },
  issn         = {0948-6143},
  journal      = {HISTOCHEMISTRY AND CELL BIOLOGY},
  keyword      = {Peroxisomes,Morpholino,Zebrafish,Zellweger syndrome,DANIO-RERIO,IMPORT RECEPTOR,DIGESTIVE-SYSTEM,BRACHYDANIO-RERIO,ZELLWEGER-SYNDROME,KIDNEY DEVELOPMENT,MEMBRANE-PROTEINS,BIOGENESIS DISORDERS,NEMATODE CAENORHABDITIS-ELEGANS,PROLIFERATOR-ACTIVATED RECEPTORS},
  language     = {eng},
  number       = {1},
  pages        = {39--51},
  title        = {Peroxisomes in zebrafish: distribution pattern and knockdown studies},
  url          = {http://dx.doi.org/10.1007/s00418-010-0712-z},
  volume       = {134},
  year         = {2010},
}

Chicago
Krysko, Olga, Mieke Stevens, Tobias Langenberg, Marc Fransen, Marc Espeel, and Myriam Baes. 2010. “Peroxisomes in Zebrafish: Distribution Pattern and Knockdown Studies.” Histochemistry and Cell Biology 134 (1): 39–51.
APA
Krysko, O., Stevens, M., Langenberg, T., Fransen, M., Espeel, M., & Baes, M. (2010). Peroxisomes in zebrafish: distribution pattern and knockdown studies. HISTOCHEMISTRY AND CELL BIOLOGY, 134(1), 39–51.
Vancouver
1.
Krysko O, Stevens M, Langenberg T, Fransen M, Espeel M, Baes M. Peroxisomes in zebrafish: distribution pattern and knockdown studies. HISTOCHEMISTRY AND CELL BIOLOGY. 2010;134(1):39–51.
MLA
Krysko, Olga, Mieke Stevens, Tobias Langenberg, et al. “Peroxisomes in Zebrafish: Distribution Pattern and Knockdown Studies.” HISTOCHEMISTRY AND CELL BIOLOGY 134.1 (2010): 39–51. Print.