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Recent progress in development of Tnt1 functional genomics platform for medicago truncatula and Lotus japonicus in Bulgaria

(2011) CURRENT GENOMICS. 12(2). p.147-152
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Abstract
Legumes, as protein-rich crops, are widely used for human food, animal feed and vegetable oil production. Over the past decade, two legume species, Medicago truncatula and Lotus japonicus, have been adopted as model legumes for genomics and physiological studies. The tobacco transposable element, Tnt1, is a powerful tool for insertional mutagenesis and gene inactivation in plants. A large collection of Tnt1-tagged lines of M. truncatula cv. Jemalong was generated during the course of the project 'GLIP': Grain Legumes Integrated Project, funded by the European Union (www.eugrainlegumes.org). In the project 'IFCOSMO': Integrated Functional and COmparative genomics Studies on the MOdel Legumes Medicago truncatula and Lotus japonicus, supported by a grant from the Ministry of Education, Youth and Science, Bulgaria, these lines are used for development of functional genomics platform of legumes in Bulgaria. This review presents recent advances in the evaluation of the M. truncatula Tnt1 mutant collection and outlines the steps that are taken in using the Tnt1-tagging for generation of a mutant collection of the second model legume L. japonicus. Both collections will provide a number of legume-specific mutants and serve as a resource for functional and comparative genomics research on legumes. Genomics technologies are expected to advance genetics and breeding of important legume crops (pea, faba bean, alfalfa and clover) in Bulgaria and worldwide.
Keywords
RETROTRANSPOSON, ARABIDOPSIS, MUTAGENESIS, HIGHER-PLANTS, DRAFT SEQUENCE, MODEL LEGUME, REVERSE GENETICS TOOL, RESOURCES, ELEMENTS, MUTANTS, Insertional mutagenesis, legume genomics, Medicago truncatula, Lotus japonicus, phenotyping, Tnt1 mutants

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Citation

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Chicago
Revalska, Miglena, Valya Vassileva, Sofie Goormachtig, Tom Van Hautegem, Pascal Ratet, and Anelia Iantcheva. 2011. “Recent Progress in Development of Tnt1 Functional Genomics Platform for Medicago Truncatula and Lotus Japonicus in Bulgaria.” Current Genomics 12 (2): 147–152.
APA
Revalska, M., Vassileva, V., Goormachtig, S., Van Hautegem, T., Ratet, P., & Iantcheva, A. (2011). Recent progress in development of Tnt1 functional genomics platform for medicago truncatula and Lotus japonicus in Bulgaria. CURRENT GENOMICS, 12(2), 147–152.
Vancouver
1.
Revalska M, Vassileva V, Goormachtig S, Van Hautegem T, Ratet P, Iantcheva A. Recent progress in development of Tnt1 functional genomics platform for medicago truncatula and Lotus japonicus in Bulgaria. CURRENT GENOMICS. 2011;12(2):147–52.
MLA
Revalska, Miglena, Valya Vassileva, Sofie Goormachtig, et al. “Recent Progress in Development of Tnt1 Functional Genomics Platform for Medicago Truncatula and Lotus Japonicus in Bulgaria.” CURRENT GENOMICS 12.2 (2011): 147–152. Print.
@article{1266405,
  abstract     = {Legumes, as protein-rich crops, are widely used for human food, animal feed and vegetable oil production. Over the past decade, two legume species, Medicago truncatula and Lotus japonicus, have been adopted as model legumes for genomics and physiological studies. The tobacco transposable element, Tnt1, is a powerful tool for insertional mutagenesis and gene inactivation in plants. A large collection of Tnt1-tagged lines of M. truncatula cv. Jemalong was generated during the course of the project 'GLIP': Grain Legumes Integrated Project, funded by the European Union (www.eugrainlegumes.org). In the project 'IFCOSMO': Integrated Functional and COmparative genomics Studies on the MOdel Legumes Medicago truncatula and Lotus japonicus, supported by a grant from the Ministry of Education, Youth and Science, Bulgaria, these lines are used for development of functional genomics platform of legumes in Bulgaria. This review presents recent advances in the evaluation of the M. truncatula Tnt1 mutant collection and outlines the steps that are taken in using the Tnt1-tagging for generation of a mutant collection of the second model legume L. japonicus. Both collections will provide a number of legume-specific mutants and serve as a resource for functional and comparative genomics research on legumes. Genomics technologies are expected to advance genetics and breeding of important legume crops (pea, faba bean, alfalfa and clover) in Bulgaria and worldwide.},
  author       = {Revalska, Miglena and Vassileva, Valya and Goormachtig, Sofie and Van Hautegem, Tom and Ratet, Pascal and Iantcheva, Anelia},
  issn         = {1389-2029},
  journal      = {CURRENT GENOMICS},
  keyword      = {RETROTRANSPOSON,ARABIDOPSIS,MUTAGENESIS,HIGHER-PLANTS,DRAFT SEQUENCE,MODEL LEGUME,REVERSE GENETICS TOOL,RESOURCES,ELEMENTS,MUTANTS,Insertional mutagenesis,legume genomics,Medicago truncatula,Lotus japonicus,phenotyping,Tnt1 mutants},
  language     = {eng},
  number       = {2},
  pages        = {147--152},
  title        = {Recent progress in development of Tnt1 functional genomics platform for medicago truncatula and Lotus japonicus in Bulgaria},
  url          = {http://dx.doi.org/10.2174/138920211795564313},
  volume       = {12},
  year         = {2011},
}

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